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Literature summary for 3.13.2.1 extracted from
- S-Adenosylhomocysteine hydrolase overexpression in HEK-293 cells: effect on intracellular adenosine levels, cell viability, and DNA methylation (2008), Cell. Physiol. Biochem., 22, 223-236.
Application
| Application | Comment | Organism |
|---|---|---|
| molecular biology | AdoHcyase overexpression results in elevated adenosine levels and decreased cell viability. Furthermore, AdoHcyase overexpressing cells show different features typical for apoptosis (cell detachment, caspase-like activity, DNA fragmentation), suggesting that cell death is due to apoptosis | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | HEK-293 cell lines stably overexpressing AdoHcyase are generated. 2-5fold overexpression of AdoHcyase is well tolerated by the cell, while greatly enhanced AdoHcyase activity results in adenosine-induced apoptosis. The fact that enhanced AdoHcyase activity does not increase transmethylation activity suggests that AdoHcyase activity under physiological conditions is not rate limiting for efficient transmethylation | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-homocysteine + H2O | Homo sapiens | - |
L-homocysteine + adenosine | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-homocysteine + H2O | - |
Homo sapiens | L-homocysteine + adenosine | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AdoHcyase | - |
Homo sapiens |
| S-adenosylhomocysteine hydrolase | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
assay at room temperature | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Homo sapiens |
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