Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.13.2.1 extracted from

  • Porcelli, M.; Moretti, M.A.; Concilio, L.; Forte, S.; Merlino, A.; Graziano, G.; Cacciapuoti, G.
    S-Adenosylhomocysteine hydrolase from the archaeon Pyrococcus furiosus: biochemical characterization and analysis of protein structure by comparative molecular modeling (2005), Proteins, 58, 815-825.
    View publication on PubMed
Search for more literature for 3.13.2.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pyrococcus furiosus
expression of mutant enzymes T60C, T60S, T60A and Q85A in Escherichia coli Homo sapiens
expression of mutant enzymesC59T, C59S and A84Q in Escherichia coli Pyrococcus furiosus

Protein Variants

Protein Variants Comment Organism
A84Q kcat/Km is 1.2fold higher then wild-type value. Ki-value for noraristeromycin is 1.2fold lower than wild-type value, Ki-value for 2-fluoronoraristeromycin is 1.3fold lower than wild-type value Pyrococcus furiosus
C59S kcat/Km is 1.5fold lower then wild-type value. Ki-value for noraristeromycin is 1.1fold higher than wild-type value, Ki-value for 2-fluoronoraristeromycin is 1.2fold lower than wild-type value Pyrococcus furiosus
C59T kcat/Km is 1.1fold higher then wild-type value. Ki-value for noraristeromycin is 1.4fold lower than wild-type value, Ki-value for 2-fluoronoraristeromycin more than 6.5fold higher than wild-type value Pyrococcus furiosus
Q85A kcat/Km is 13.45fold lower then wild-type value. Ki-value for noraristeromycin is 4.46fold higher than wild-type value, Ki-value for 2-fluoronoraristeromycin is 5.6fold higher than wild-type value Homo sapiens
T60A kcat/Km is 1.23 fold lower then wild-type value. Ki-value for noraristeromycin is 3.3fold lower than wild-type value, Ki-value for 2-fluoronoraristeromycin is 23fold lower than wild-type value Homo sapiens
T60C kcat/Km is 2.3fold lower then wild-type value. Ki-value for noraristeromycin is 1.2fold higher than wild-type value, Ki-value for 2-fluoronoraristeromycin 11fold lower than wild-type value Homo sapiens
T60S kcat/Km is 1.6fold higher then wild-type value. Ki-value for noraristeromycin is 2.3fold lower than wild-type value, Ki-value for 2-fluoronoraristeromycin is 50fold lower than wild-type value Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
2-fluoronoraristeromycin
-
 Homo sapiens
2-fluoronoraristeromycin
-
 Pyrococcus furiosus
dithiothreitol 0.8 M, reduces activity Pyrococcus furiosus
noraristeromycin
-
 Homo sapiens
noraristeromycin
-
 Pyrococcus furiosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00024
-
 S-adenosyl-DL-homocysteine 30°C, pH 7.6, mutant enzyme A84Q Pyrococcus furiosus
0.00025
-
 S-adenosyl-DL-homocysteine 30°C, pH 7.6, mutant enzyme C59T Pyrococcus furiosus
0.00029
-
 S-adenosyl-DL-homocysteine 30°C, pH 7.6, wild-type enzyme Pyrococcus furiosus
0.00039
-
 S-adenosyl-DL-homocysteine 30°C, pH 7.6, mutant enzyme C59S Pyrococcus furiosus
0.0007
-
 S-adenosyl-DL-homocysteine 30°C, pH 7.6, mutant enzyme T60S Homo sapiens
0.00129
-
 S-adenosyl-DL-homocysteine 30°C, pH 7.6, mutant enzyme T60A Homo sapiens
0.0014
-
 S-adenosyl-DL-homocysteine 30°C, pH 7.6, wild-type enzyme Homo sapiens
0.00172
-
 S-adenosyl-DL-homocysteine 30°C, pH 7.6, mutant enzyme T60C Homo sapiens
0.0048
-
 adenosine 80°C, pH 7.4 Pyrococcus furiosus
0.00555
-
 S-adenosyl-DL-homocysteine 30°C, pH 7.6, mutant enzyme Q85A Homo sapiens
0.014
-
 S-adenosyl-DL-homocysteine 80°C, pH 7.4 Pyrococcus furiosus
0.14
-
 DL-homocysteine 80°C, pH 7.4 Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
 4 * 47000, SDS-PAGE Pyrococcus furiosus
190000
-
 gel filtration Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-homocysteine + H2O Pyrococcus furiosus the enzyme catalyzes the breakdown of S-adenosylhomocysteine, a powerful inhibitor of most transmethylation reactions, to adenosine and L-homocysteine L-homocysteine + adenosine
-
 r

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Pyrococcus furiosus
-
-
-
Pyrococcus furiosus P50251
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens
-
 Pyrococcus furiosus
recombinant Pyrococcus furiosus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.033
-
-
 Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DL-homocysteine + adenosine
-
 Pyrococcus furiosus S-adenosyl-DL-homocysteine
-
 ?
S-adenosyl-DL-homocysteine + H2O
-
 Homo sapiens DL-homocysteine + adenosine
-
 ?
S-adenosyl-DL-homocysteine + H2O
-
 Pyrococcus furiosus DL-homocysteine + adenosine
-
 ?
S-adenosyl-L-homocysteine + H2O
-
 Pyrococcus furiosus L-homocysteine + adenosine
-
 r
S-adenosyl-L-homocysteine + H2O the enzyme catalyzes the breakdown of S-adenosylhomocysteine, a powerful inhibitor of most transmethylation reactions, to adenosine and L-homocysteine Pyrococcus furiosus L-homocysteine + adenosine
-
 r

Subunits

Subunits Comment Organism
homotetramer 4 * 47000, SDS-PAGE Pyrococcus furiosus
tetramer 4 * 47000, SDS-PAGE Pyrococcus furiosus

Synonyms

Synonyms Comment Organism
AdoHcy hydrolase
-
 Pyrococcus furiosus
AdoHcyHD
-
 Pyrococcus furiosus
HsSAHH
-
 Homo sapiens
PF0343
-
 Pyrococcus furiosus
PfAdoHcyHD
-
 Pyrococcus furiosus
PfSAHH
-
 Pyrococcus furiosus
S-adenosylhomocysteine hydrolase
-
 Pyrococcus furiosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
 assay at Pyrococcus furiosus
95
-
-
 Pyrococcus furiosus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
75 105 75°C: about 50% of maximal activity, 105°C: about 45% of maximal activity Pyrococcus furiosus
75 105 75°C: about 50% of maximalm activity, 105°C: about 45% of maximal activity Pyrococcus furiosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
 1 h, stable Pyrococcus furiosus
90
-
 1 h, no loss of activity Pyrococcus furiosus
95
-
 half-life: 50 min Pyrococcus furiosus
98
-
 melting temperature Pyrococcus furiosus
100
-
 half-life: 5 min Pyrococcus furiosus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.053
-
 S-adenosyl-DL-homocysteine 30°C, pH 7.6, mutant enzyme C59S Pyrococcus furiosus
0.054
-
 S-adenosyl-DL-homocysteine 30°C, pH 7.6, mutant enzyme C59T Pyrococcus furiosus
0.057
-
 S-adenosyl-DL-homocysteine 30°C, pH 7.6, mutant enzyme A84Q Pyrococcus furiosus
0.058
-
 S-adenosyl-DL-homocysteine 30°C, pH 7.6, wild-type enzyme Pyrococcus furiosus
1
-
 S-adenosyl-DL-homocysteine 30°C, pH 7.6, mutant enzyme Q85A Homo sapiens
1.87
-
 S-adenosyl-DL-homocysteine 30°C, pH 7.6, mutant enzyme T60C Homo sapiens
2.55
-
 S-adenosyl-DL-homocysteine 30°C, pH 7.6, mutant enzyme T60A Homo sapiens
2.8
-
 S-adenosyl-DL-homocysteine 30°C, pH 7.6, mutant enzyme T60S Homo sapiens
3.45
-
 S-adenosyl-DL-homocysteine 30°C, pH 7.6, wild-type enzyme Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
-
 Pyrococcus furiosus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000024
-
 noraristeromycin 30°C, pH 7.6, mutant enzyme C59T Pyrococcus furiosus
0.000028
-
 noraristeromycin 30°C, pH 7.6, mutant enzyme T60A Homo sapiens
0.000029
-
 noraristeromycin 30°C, pH 7.6, mutant enzyme A84Q Pyrococcus furiosus
0.000034
-
 noraristeromycin 30°C, pH 7.6, wild-type enzyme Pyrococcus furiosus
0.000036
-
 noraristeromycin 30°C, pH 7.6, mutant enzyme C59S Pyrococcus furiosus
0.000039
-
 noraristeromycin 30°C, pH 7.6, mutant enzyme T60S Homo sapiens
0.00006
-
 2-fluoronoraristeromycin 30°C, pH 7.6, mutant enzyme T60S Homo sapiens
0.000092
-
 noraristeromycin 30°C, pH 7.6, wild-type enzyme Homo sapiens
0.0001
-
 noraristeromycin 30°C, pH 7.6, mutant enzyme T60C Homo sapiens
0.000116
-
 2-fluoronoraristeromycin 30°C, pH 7.6, mutant enzyme A84Q Pyrococcus furiosus
0.000129
-
 2-fluoronoraristeromycin 30°C, pH 7.6, mutant enzyme T60A Homo sapiens
0.00013
-
 2-fluoronoraristeromycin 30°C, pH 7.6, mutant enzyme C59S Pyrococcus furiosus
0.000154
-
 2-fluoronoraristeromycin 30°C, pH 7.6, wild-type enzyme Pyrococcus furiosus
0.000272
-
 2-fluoronoraristeromycin 30°C, pH 7.6, mutant enzyme T60C Homo sapiens
0.00041
-
 noraristeromycin 30°C, pH 7.6, mutant enzyme Q85A Homo sapiens
0.003
-
 2-fluoronoraristeromycin 30°C, pH 7.6, wild-type enzyme Homo sapiens
0.0168
-
 2-fluoronoraristeromycin 30°C, pH 7.6, mutant enzyme Q85A Homo sapiens

pI Value

Organism Comment pI Value Maximum pI Value
Pyrococcus furiosus isoelectric focusing, pH-range 3-10 and 4.0-6.5
-
 5.1
Pyrococcus furiosus isoelectric focusing, pH ranges 3–10 and 4.0–6.5
-
 5.1

General Information

General Information Comment Organism
physiological function the enzyme catalyzes the breakdown of S-adenosylhomocysteine, a powerful inhibitor of most transmethylation reactions, to adenosine and L-homocysteine Pyrococcus furiosus