Literature summary for 3.13.2.1 extracted from

Porcelli, M.; Fusco, S.; Inizio, T.; Zappia, V.; Cacciapuoti, G.
Expression, purification, and characterization of recombinant S-adenosylhomocysteine hydrolase from the thermophilic archaeon Sulfolobus solfactorius (2000), Protein Expr. Purif., 18, 27-35.

Search for more literature for 3.13.2.1

Application

Application	Comment	Organism
medicine	antiviral drug design	Saccharolobus solfataricus

Cloned(Commentary)

Cloned (Comment)	Organism
into Escherichia coli	Saccharolobus solfataricus

General Stability

General Stability	Organism
unusual stability in protein denaturants and detergents, complete recombinant enzyme	Saccharolobus solfataricus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.014	-	S-adenosyl-L-homocysteine	complete recombinant enzyme	Saccharolobus solfataricus
0.016	-	S-adenosyl-L-homocysteine	truncated recombinant enzyme	Saccharolobus solfataricus
0.04	-	adenosine	complete recombinant enzyme	Saccharolobus solfataricus
0.042	-	adenosine	truncated recombinant enzyme	Saccharolobus solfataricus
0.148	-	L-homocysteine	truncated recombinant enzyme	Saccharolobus solfataricus
0.152	-	L-homocysteine	complete recombinant enzyme	Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
190000	-	recombinant enzymes, gel filtration	Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
S-adenosyl-L-homocysteine + H2O	Saccharolobus solfataricus	-	adenosine + L-homocysteine	-	r

Organism

Organism	UniProt	Comment	Textmining
Saccharolobus solfataricus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
truncated recombinant enzyme: 71.7fold, complete recombinant enzyme: to homogeneity	Saccharolobus solfataricus

Reaction

Reaction	Comment	Organism	Reaction ID
S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine	reaction equilibrium is in vitro strongly in direction of synthesis of S-adenosyl-L-homocysteine	Saccharolobus solfataricus	a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.163	-	complete recombinant enzyme	Saccharolobus solfataricus
0.165	-	truncated recombinant enzyme, wild-type	Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-homocysteine + H2O	-	Saccharolobus solfataricus	adenosine + L-homocysteine	-	r

Subunits

Subunits	Comment	Organism
tetramer	homotetramer, recombinant enzymes, SDS-PAGE	Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
75	-	recombinant enzymes	Saccharolobus solfataricus

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
additional information	-	truncated recombinant enzyme	Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
88	-	melting temperature, truncated recombinant enzyme	Saccharolobus solfataricus
95	-	melting temperature, complete recombinant enzyme	Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	complete recombinant enzyme	Saccharolobus solfataricus

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Saccharolobus solfataricus

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Release 2023.1 (January 2023)