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Literature summary for 3.13.2.1 extracted from
- Purification and properties of S-adenosyl-L-homocysteine hydrolase from leaves of spinach beet (1976), Arch. Biochem. Biophys., 172, 135-142.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| adenosine deaminase | - |
Beta vulgaris |
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | study of methylation reactions | Beta vulgaris |
General Stability
| General Stability | Organism |
|---|---|
| unstable in solutions containing low level of protein, stabilization with bovine serum albumin | Beta vulgaris |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| adenine | - |
Beta vulgaris |  |
| DL-homocysteine | - |
Beta vulgaris | |
| S-adenosyl-L-methionine | - |
Beta vulgaris | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0128 | - |
adenosine | - |
Beta vulgaris | |
| 0.041 | - |
S-adenosyl-L-homocysteine | - |
Beta vulgaris | |
| 1.2 | - |
L-homocysteine | - |
Beta vulgaris | |
| 2.2 | - |
DL-homocysteine | - |
Beta vulgaris | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-homocysteine + H2O | Beta vulgaris | - |
adenosine + L-homocysteine | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Beta vulgaris | - |
L. ssp. vulgaris | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 100fold | Beta vulgaris |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine | reaction equilibrium is in vitro strongly in direction of synthesis of S-adenosyl-L-homocysteine | Beta vulgaris |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Beta vulgaris | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.84 | - |
- |
Beta vulgaris |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, with 5 mM beta-mercaptoethanol and 2 mg/ml bovine serum albumin, stable for several months | Beta vulgaris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DL-homocysteine + 3-deazaadenosine | - |
Beta vulgaris | ? | - |
r | |
| DL-homocysteine + adenosine | - |
Beta vulgaris | S-adenosyl-DL-homocysteine | - |
r | |
| S-adenosyl-L-homocysteine + H2O | - |
Beta vulgaris | adenosine + L-homocysteine | - |
r | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
synthesis and hydrolysis of S-adenosyl-L-homocysteine | Beta vulgaris |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Beta vulgaris |
| 6.5 | 10 | more than 50% of activity | Beta vulgaris |
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