Literature summary for 3.13.2.1 extracted from

Hohman, R.J.; Guitton, M.C.; Veron, M.
Purification of S-adenosyl-L-homocysteine hydrolase from Dictyostelium discoideum: Reversible inactivation by cAMP and 2 -deoxyadenosine (1984), Arch. Biochem. Biophys., 233, 785-795.

Search for more literature for 3.13.2.1

Activating Compound

Activating Compound	Comment	Organism	Structure
NAD+	25% activation, restores activity of 2'-deoxyadenosine-, ATP, AMP or cAMP-treated enzyme, stabilizes quaternary structure	Oryctolagus cuniculus
NAD+	25% activation, restores activity of 2'-deoxyadenosine-, ATP, AMP or cAMP-treated enzyme, stabilizes quaternary structure	Dictyostelium discoideum

Inhibitors

Inhibitors	Comment	Organism	Structure
2'-deoxyadenosine	reversible	Dictyostelium discoideum
2'-deoxyadenosine	-	Oryctolagus cuniculus
adenosine-5'-monophosphate	-	Dictyostelium discoideum
adenosine-5'-monophosphate	-	Oryctolagus cuniculus
ATP	-	Dictyostelium discoideum
ATP	-	Oryctolagus cuniculus
cAMP	reversible	Dictyostelium discoideum
cAMP	-	Oryctolagus cuniculus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0004	-	adenosine	-	Dictyostelium discoideum
0.006	-	S-adenosyl-L-homocysteine	-	Dictyostelium discoideum

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Dictyostelium discoideum	5829	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
47000	-	4 * 47000, SDS-PAGE	Dictyostelium discoideum
190000	-	gel filtration	Dictyostelium discoideum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
S-adenosyl-L-homocysteine + H2O	Oryctolagus cuniculus	-	adenosine + L-homocysteine	-	r
S-adenosyl-L-homocysteine + H2O	Dictyostelium discoideum	-	adenosine + L-homocysteine	-	r

Organism

Organism	UniProt	Comment	Textmining
Dictyostelium discoideum	-	-	-
Oryctolagus cuniculus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
52fold to homogeneity	Dictyostelium discoideum

Source Tissue

Source Tissue	Comment	Organism	Textmining
erythrocyte	-	Oryctolagus cuniculus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.3	-	-	Dictyostelium discoideum

Storage Stability

Storage Stability	Organism
4°C, sterilized enzyme at a concentration of 2-4 mg/ml, in buffer C, stable for at least 2 months	Dictyostelium discoideum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-homocysteine + H2O	-	Oryctolagus cuniculus	adenosine + L-homocysteine	-	r
S-adenosyl-L-homocysteine + H2O	-	Dictyostelium discoideum	adenosine + L-homocysteine	-	r

Subunits

Subunits	Comment	Organism
tetramer	4 * 47000, SDS-PAGE	Dictyostelium discoideum

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
25	-	irreversible inactivation in absence of ligand	Dictyostelium discoideum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	hydrolysis of S-adenosyl-L-homocysteine	Dictyostelium discoideum
8	-	synthesis of S-adenosyl-L-homocysteine	Dictyostelium discoideum

pH Range

pH Minimum	pH Maximum	Comment	Organism
additional information	-	-	Dictyostelium discoideum

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Release 2023.1 (January 2023)