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Literature summary for 3.13.1.7 extracted from

  • Smeulders, M.J.; Pol, A.; Venselaar, H.; Barends, T.R.; Hermans, J.; Jetten, M.S.; Op den Camp, H.J.
    Bacterial CS2 hydrolases from Acidithiobacillus thiooxidans strains are homologous to the archaeal catenane CS2 hydrolase (2013), J. Bacteriol., 195, 4046-4056 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.13.1.7

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.014
-
 carbonyl sulfide at pH 7.0 and 30°C Acidithiobacillus thiooxidans
0.046
-
 carbonyl disulfide at pH 7.0 and 30°C Acidithiobacillus thiooxidans
0.074
-
 carbonyl sulfide at pH 7.0 and 30°C Acidithiobacillus thiooxidans
0.093
-
 carbonyl disulfide at pH 7.0 and 30°C Acidithiobacillus thiooxidans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
192000
-
 octamer, gel filtration Acidithiobacillus thiooxidans
384000
-
 hexadecamer, gel filtration Acidithiobacillus thiooxidans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
carbonyl sulfide + H2O Acidithiobacillus thiooxidans
-
 hydrogen sulfide + CO2
-
 ?
carbonyl sulfide + H2O Acidithiobacillus thiooxidans S1p
-
 hydrogen sulfide + CO2
-
 ?
carbonyl sulfide + H2O Acidithiobacillus thiooxidans Sts 4-3
-
 hydrogen sulfide + CO2
-
 ?
carbonyl sulfide + H2O Acidithiobacillus thiooxidans 2Bp
-
 hydrogen sulfide + CO2
-
 ?
carbonyl sulfide + H2O Acidithiobacillus thiooxidans BBW1
-
 hydrogen sulfide + CO2
-
 ?
carbonyl sulfide + H2O Acidithiobacillus thiooxidans G8
-
 hydrogen sulfide + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Acidithiobacillus thiooxidans
-
-
-
Acidithiobacillus thiooxidans 2Bp
-
-
-
Acidithiobacillus thiooxidans BBW1
-
-
-
Acidithiobacillus thiooxidans G8
-
-
-
Acidithiobacillus thiooxidans S1p
-
-
-
Acidithiobacillus thiooxidans Sts 4-3
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carbonyl disulfide + H2O
-
 Acidithiobacillus thiooxidans carbonyl sulfide + CO2
-
 ?
carbonyl disulfide + H2O
-
 Acidithiobacillus thiooxidans S1p carbonyl sulfide + CO2
-
 ?
carbonyl disulfide + H2O
-
 Acidithiobacillus thiooxidans Sts 4-3 carbonyl sulfide + CO2
-
 ?
carbonyl disulfide + H2O
-
 Acidithiobacillus thiooxidans 2Bp carbonyl sulfide + CO2
-
 ?
carbonyl disulfide + H2O
-
 Acidithiobacillus thiooxidans BBW1 carbonyl sulfide + CO2
-
 ?
carbonyl disulfide + H2O
-
 Acidithiobacillus thiooxidans G8 carbonyl sulfide + CO2
-
 ?
carbonyl sulfide + H2O
-
 Acidithiobacillus thiooxidans hydrogen sulfide + CO2
-
 ?
carbonyl sulfide + H2O
-
 Acidithiobacillus thiooxidans S1p hydrogen sulfide + CO2
-
 ?
carbonyl sulfide + H2O
-
 Acidithiobacillus thiooxidans Sts 4-3 hydrogen sulfide + CO2
-
 ?
carbonyl sulfide + H2O
-
 Acidithiobacillus thiooxidans 2Bp hydrogen sulfide + CO2
-
 ?
carbonyl sulfide + H2O
-
 Acidithiobacillus thiooxidans BBW1 hydrogen sulfide + CO2
-
 ?
carbonyl sulfide + H2O
-
 Acidithiobacillus thiooxidans G8 hydrogen sulfide + CO2
-
 ?
additional information the enzyme cannot convert CO2 to HCO3- Acidithiobacillus thiooxidans ?
-
 ?
additional information the enzyme cannot convert CO2 to HCO3- Acidithiobacillus thiooxidans S1p ?
-
 ?
additional information the enzyme cannot convert CO2 to HCO3- Acidithiobacillus thiooxidans Sts 4-3 ?
-
 ?
additional information the enzyme cannot convert CO2 to HCO3- Acidithiobacillus thiooxidans 2Bp ?
-
 ?
additional information the enzyme cannot convert CO2 to HCO3- Acidithiobacillus thiooxidans BBW1 ?
-
 ?
additional information the enzyme cannot convert CO2 to HCO3- Acidithiobacillus thiooxidans G8 ?
-
 ?

Subunits

Subunits Comment Organism
hexadecamer octamer or hexadecamer Acidithiobacillus thiooxidans
octamer
-
 Acidithiobacillus thiooxidans
octamer octamer or hexadecamer Acidithiobacillus thiooxidans

Synonyms

Synonyms Comment Organism
COS hydrolase
-
 Acidithiobacillus thiooxidans
CS2 hydrolase
-
 Acidithiobacillus thiooxidans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
8400
-
 carbonyl disulfide at pH 7.0 and 30°C Acidithiobacillus thiooxidans
11000
-
 carbonyl sulfide at pH 7.0 and 30°C Acidithiobacillus thiooxidans
64000
-
 carbonyl disulfide at pH 7.0 and 30°C Acidithiobacillus thiooxidans
160000
-
 carbonyl sulfide at pH 7.0 and 30°C Acidithiobacillus thiooxidans