Literature summary for 3.13.1.7 extracted from

Ogawa, T.; Noguchi, K.; Saito, M.; Nagahata, Y.; Kato, H.; Ohtaki, A.; Nakayama, H.; Dohmae, N.; Matsushita, Y.; Odaka, M.; Yohda, M.; Nyunoya, H.; Katayama, Y.
Carbonyl sulfide hydrolase from Thiobacillus thioparus strain THI115 is one of the beta-carbonic anhydrase family enzymes (2013), J. Am. Chem. Soc., 135, 3818-3825 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Thiobacillus thioparus

Crystallization (Commentary)

Crystallization (Comment)	Organism
enzyme complexed with SCN-, hanging drop vapor diffusion method, using 1.2 M (NH4)2SO4, 0.2 M NaCl, 30% glycerol, 0.1 M Tris-HCl, pH 8.5	Thiobacillus thioparus

Inhibitors

Inhibitors	Comment	Organism	Structure
SCN-	70% and 20% residual activity at 0.01 and 0.1 mM, respectively	Thiobacillus thioparus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.06	-	carbonyl sulfide	at pH 8.5 and 30°C	Thiobacillus thioparus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	contains a zinc ion in its catalytic site	Thiobacillus thioparus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
carbonyl sulfide + H2O	Thiobacillus thioparus	-	hydrogen sulfide + CO2	-	?
carbonyl sulfide + H2O	Thiobacillus thioparus THI115	-	hydrogen sulfide + CO2	-	?

Organism

Organism	UniProt	Comment	Textmining
Thiobacillus thioparus	H1AAP2	-	-
Thiobacillus thioparus THI115	H1AAP2	-	-

Purification (Commentary)

Purification (Comment)	Organism
column chromatography	Thiobacillus thioparus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
carbonyl sulfide + H2O	-	Thiobacillus thioparus	hydrogen sulfide + CO2	-	?
carbonyl sulfide + H2O	-	Thiobacillus thioparus THI115	hydrogen sulfide + CO2	-	?

Subunits

Subunits	Comment	Organism
homotetramer	4 * 27000, SDS-PAGE	Thiobacillus thioparus
homotetramer	4 * 23400, calculated from amino acid sequence	Thiobacillus thioparus

Synonyms

Synonyms	Comment	Organism
COSase	-	Thiobacillus thioparus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
58	-	carbonyl sulfide	at pH 8.5 and 30°C	Thiobacillus thioparus

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
960	-	carbonyl sulfide	at pH 8.5 and 30°C	Thiobacillus thioparus

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Release 2023.1 (January 2023)