Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | inability of the small KCN and CF3SO2NH2 to inhibit CS2 hydrolase | Acidianus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics and thermodynamics, overview | Acidianus sp. | |
0.178 | - |
carbon disulfide | pH 7.5, 50°C, ring form enzyme | Acidianus sp. | |
0.193 | - |
carbon disulfide | pH 7.5, 50°C, catenane form enzyme | Acidianus sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | required | Acidianus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
carbon disulfide + 2 H2O | Acidianus sp. | - |
CO2 + 2 hydrogen sulfide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acidianus sp. | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carbon disulfide + 2 H2O | - |
Acidianus sp. | CO2 + 2 hydrogen sulfide | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the enzyme exists in solution as a mixture of unique hexadecameric catenane and octameric ring forms, multi-angle laser light scattering and native mass spectrometric analyses, overview. Four of the sixteen active sites of the catenane can be partially blocked due to the packed perpendicular arrangement of the two interlocked rings | Acidianus sp. |
Synonyms | Comment | Organism |
---|---|---|
CS2 hydrolase | - |
Acidianus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
assay at | Acidianus sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
48 | - |
carbon disulfide | pH 7.5, 50°C, catenane form enzyme | Acidianus sp. | |
72 | - |
carbon disulfide | pH 7.5, 50°C, ring form enzyme | Acidianus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Acidianus sp. |
General Information | Comment | Organism |
---|---|---|
additional information | the ring form of CS2 hydrolase possesses higher enzyme efficiency (per monomer) than the catenane form, whereas the catenane form is overall more active (per assembly) | Acidianus sp. |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
249 | - |
carbon disulfide | pH 7.5, 50°C, catenane form enzyme | Acidianus sp. | |
409 | - |
carbon disulfide | pH 7.5, 50°C, ring form enzyme | Acidianus sp. |