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Literature summary for 3.13.1.5 extracted from

  • Van Eldijk, M.; Pieters, B.; Mikhailov, V.; Robinson, C.; Van Hest, J.; Mecinovic, J.
    Catenane versus ring: do both assemblies of CS2 hydrolase exhibit the same stability and catalytic activity? (2014), Chem. Sci., 5, 2879-2884.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
additional information inability of the small KCN and CF3SO2NH2 to inhibit CS2 hydrolase Acidianus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics and thermodynamics, overview Acidianus sp.
0.178
-
carbon disulfide pH 7.5, 50°C, ring form enzyme Acidianus sp.
0.193
-
carbon disulfide pH 7.5, 50°C, catenane form enzyme Acidianus sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ required Acidianus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
carbon disulfide + 2 H2O Acidianus sp.
-
CO2 + 2 hydrogen sulfide
-
?

Organism

Organism UniProt Comment Textmining
Acidianus sp.
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carbon disulfide + 2 H2O
-
Acidianus sp. CO2 + 2 hydrogen sulfide
-
?

Subunits

Subunits Comment Organism
More the enzyme exists in solution as a mixture of unique hexadecameric catenane and octameric ring forms, multi-angle laser light scattering and native mass spectrometric analyses, overview. Four of the sixteen active sites of the catenane can be partially blocked due to the packed perpendicular arrangement of the two interlocked rings Acidianus sp.

Synonyms

Synonyms Comment Organism
CS2 hydrolase
-
Acidianus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
assay at Acidianus sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
48
-
carbon disulfide pH 7.5, 50°C, catenane form enzyme Acidianus sp.
72
-
carbon disulfide pH 7.5, 50°C, ring form enzyme Acidianus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Acidianus sp.

General Information

General Information Comment Organism
additional information the ring form of CS2 hydrolase possesses higher enzyme efficiency (per monomer) than the catenane form, whereas the catenane form is overall more active (per assembly) Acidianus sp.

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
249
-
carbon disulfide pH 7.5, 50°C, catenane form enzyme Acidianus sp.
409
-
carbon disulfide pH 7.5, 50°C, ring form enzyme Acidianus sp.