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Literature summary for 3.13.1.5 extracted from

  • Smeulders, M.J.; Pol, A.; Zandvoort, M.H.; Jetten, M.S.; Op den Camp, H.J.
    Diversity and ecophysiology of new isolates of extremely acidophilic CS2-converting Acidithiobacillus strains (2013), Appl. Environ. Microbiol., 79, 6784-6794.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Michaelis-Menten kinetics Acidithiobacillus thiooxidans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
carbon disulfide + 2 H2O Acidithiobacillus thiooxidans
-
CO2 + 2 hydrogen sulfide
-
?
carbon disulfide + 2 H2O Acidithiobacillus caldus
-
CO2 + 2 hydrogen sulfide
-
?
carbon disulfide + 2 H2O Acidithiobacillus thiooxidans S1p
-
CO2 + 2 hydrogen sulfide
-
?
carbon disulfide + 2 H2O Acidithiobacillus thiooxidans Sts 4-3
-
CO2 + 2 hydrogen sulfide
-
?
carbon disulfide + 2 H2O Acidithiobacillus thiooxidans 2Bp
-
CO2 + 2 hydrogen sulfide
-
?
carbon disulfide + 2 H2O Acidithiobacillus caldus 8584
-
CO2 + 2 hydrogen sulfide
-
?
carbon disulfide + 2 H2O Acidithiobacillus thiooxidans BBW1
-
CO2 + 2 hydrogen sulfide
-
?
carbon disulfide + 2 H2O Acidithiobacillus thiooxidans G8
-
CO2 + 2 hydrogen sulfide
-
?

Organism

Organism UniProt Comment Textmining
Acidithiobacillus caldus
-
-
-
Acidithiobacillus caldus 8584
-
-
-
Acidithiobacillus thiooxidans
-
-
-
Acidithiobacillus thiooxidans 2Bp
-
-
-
Acidithiobacillus thiooxidans BBW1
-
-
-
Acidithiobacillus thiooxidans G8
-
-
-
Acidithiobacillus thiooxidans S1p
-
-
-
Acidithiobacillus thiooxidans Sts 4-3
-
-
-
no activity in Acidithiobacillus albertensis strain DSM 14366
-
-
-
no activity in Acidithiobacillus ferrooxidans strain DSM 14882
-
-
-
no activity in Acidithiobacillus thiooxidans strains DSM504 and DSM14887
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information small, smooth, domed colony growth at pH 0.5 up to pH 5.0, optimum at pH 1.0 Acidithiobacillus thiooxidans
-
additional information small, smooth, domed colony growth at pH 0.5 up to pH 6.0, optimum at pH 1.0 Acidithiobacillus thiooxidans
-
additional information white, dry, compact colony growth at pH 1.0-6.0, optimum at pH 2.0 Acidithiobacillus thiooxidans
-
additional information white, dry, spreading colony growth at pH 0.5-3.5, optimum at pH 1.0-1.5 Acidithiobacillus thiooxidans
-
additional information White, dry, spreading colony growth at pH 0.5-6.0, optimum at pH 1.5 Acidithiobacillus thiooxidans
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carbon disulfide + 2 H2O
-
Acidithiobacillus thiooxidans CO2 + 2 hydrogen sulfide
-
?
carbon disulfide + 2 H2O
-
Acidithiobacillus caldus CO2 + 2 hydrogen sulfide
-
?
carbon disulfide + 2 H2O
-
Acidithiobacillus thiooxidans S1p CO2 + 2 hydrogen sulfide
-
?
carbon disulfide + 2 H2O
-
Acidithiobacillus thiooxidans Sts 4-3 CO2 + 2 hydrogen sulfide
-
?
carbon disulfide + 2 H2O
-
Acidithiobacillus thiooxidans 2Bp CO2 + 2 hydrogen sulfide
-
?
carbon disulfide + 2 H2O
-
Acidithiobacillus caldus 8584 CO2 + 2 hydrogen sulfide
-
?
carbon disulfide + 2 H2O
-
Acidithiobacillus thiooxidans BBW1 CO2 + 2 hydrogen sulfide
-
?
carbon disulfide + 2 H2O
-
Acidithiobacillus thiooxidans G8 CO2 + 2 hydrogen sulfide
-
?

Synonyms

Synonyms Comment Organism
CS2 hydrolase
-
Acidithiobacillus thiooxidans
CS2 hydrolase
-
Acidithiobacillus caldus
CS2 hydrolase
-
no activity in Acidithiobacillus ferrooxidans strain DSM 14882
CS2 hydrolase
-
no activity in Acidithiobacillus albertensis strain DSM 14366
CS2 hydrolase
-
no activity in Acidithiobacillus thiooxidans strains DSM504 and DSM14887

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Acidithiobacillus thiooxidans

Expression

Organism Comment Expression
Acidithiobacillus thiooxidans CS2 starvation reduces the enzyme activity and H2S production within 24 h, profile overview. The activity can be regained within 4 h after end of starvation down