Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Rhodococcus erythropolis |
Protein Variants | Comment | Organism |
---|---|---|
Q65H | increase in thermostability, decrease in catalytic activity | Rhodococcus erythropolis |
Y63A | increase in catalytic activity | Rhodococcus erythropolis |
Y63F | increase in catalytic activity, similar thermostability as wild-type | Rhodococcus erythropolis |
Y63F/Q65H | increase in thermostability without loss of catalytic activity or affinity for substrate | Rhodococcus erythropolis |
Y63K | decrease in activity | Rhodococcus erythropolis |
Y63S | affinity for the substrate is about 7-fold lower than that of the wild-type enzyme | Rhodococcus erythropolis |
Y63W | activity similar to wild-type | Rhodococcus erythropolis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0056 | - |
2'-hydroxybiphenyl-2-sulfinate | mutant Y63F, pH 7.0, 28°C | Rhodococcus erythropolis | |
0.0082 | - |
2'-hydroxybiphenyl-2-sulfinate | wild-type, pH 7.0, 28°C | Rhodococcus erythropolis | |
0.0098 | - |
2'-hydroxybiphenyl-2-sulfinate | mutant Y63F/Q65H, pH 7.0, 28°C | Rhodococcus erythropolis | |
0.0556 | - |
2'-hydroxybiphenyl-2-sulfinate | mutant Y63S, pH 7.0, 28°C | Rhodococcus erythropolis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus erythropolis | P54997 | strain KA2-5-1 | - |
Rhodococcus erythropolis IGTS8 / ATCC 53968 | P54997 | strain KA2-5-1 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8.3 | - |
mutant Y63K, pH 7.0, 28°C | Rhodococcus erythropolis |
21 | - |
mutant Q65H, pH 7.0, 28°C | Rhodococcus erythropolis |
26.8 | - |
wild-type, pH 7.0, 28°C | Rhodococcus erythropolis |
29.2 | - |
mutant Y63W, pH 7.0, 28°C | Rhodococcus erythropolis |
34.1 | - |
mutant Y63F/Q65H, pH 7.0, 28°C | Rhodococcus erythropolis |
43 | - |
mutant Y63F, pH 7.0, 28°C | Rhodococcus erythropolis |
49.1 | - |
mutant Y63A, pH 7.0, 28°C | Rhodococcus erythropolis |
61.1 | - |
mutant Y63S, pH 7.0, 28°C | Rhodococcus erythropolis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'-hydroxybiphenyl-2-sulfinate + H2O | - |
Rhodococcus erythropolis | 2-hydroxybiphenyl + sulfite | - |
? | |
2'-hydroxybiphenyl-2-sulfinate + H2O | - |
Rhodococcus erythropolis IGTS8 / ATCC 53968 | 2-hydroxybiphenyl + sulfite | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
30 min, wild-type, almost complete loss of activity, mutant Y63F/Q65H, 90% residual activity | Rhodococcus erythropolis |
40 | - |
30 min,mutant Y63F/Q65H, 90% residual activity | Rhodococcus erythropolis |