2-(2'-Hydroxyphenyl)benzene sulfinate desulfinase from the thermophilic desulfurizing bacterium Paenibacillus sp. strain A11-2: purification and characterization

Konishi, J.; Maruhashi, K.; Appl. Microbiol. Biotechnol. 62, 356-361 (2003)

Data extracted from this reference:

Activating Compound

Commentary

Organism

Structure

Mg2+

1.23fold activation at 1 mM

Paenibacillus sp.

Inhibitors

Commentary

Organism

Structure

2-Hydroxybiphenyl

30% inhibition at 5 mM, the OH-group is important for the inhibitory effect

Paenibacillus sp.

Cu2+

67% inhibition at 1 mM

Paenibacillus sp.

DTNB

86% inhibition at 0.1 mM

Paenibacillus sp.

Fe2+

44% inhibition at 1 mM

Paenibacillus sp.

iodoacetic acid

95% inhibition at 1 mM

Paenibacillus sp.

Mn2+

71% inhibition at 1 mM

Paenibacillus sp.

additional information

no inhibition by 1,10-phenanthroline and EDTA, no inhibition by Co2+, Ca2+, and Mg2+, no inhibition by biphenyl, 2-methylbiphenyl, and 2-methoxybiphenyl

Paenibacillus sp.

N-ethylmaleimide

complete inhibition at 1 mM

Paenibacillus sp.

Ni2+

24% inhibition at 1 mM

Paenibacillus sp.

o-hydroxystyrene

product inhibition, 80% inhibition at 5 mM, the OH-group is important for the inhibitory effect

Paenibacillus sp.

p-chloromercuribenzoate

92% inhibition at 0.1 mM

Paenibacillus sp.

Zn2+

98% inhibition at 1 mM

Paenibacillus sp.

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.33

-

2-(2-hydroxyphenyl)benzenesulfinate

pH 7.0, 50°C

Paenibacillus sp.

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

2-(2'-hydroxyphenyl)benzenesulfinate + H2O

Paenibacillus sp.

final step of the desulfurozation of dibenzothiophene

2-hydroxybiphenyl + sulfite

-

Paenibacillus sp.

ir

2-(2'-hydroxyphenyl)benzenesulfinate + H2O

Paenibacillus sp. A11-2

final step of the desulfurozation of dibenzothiophene

2-hydroxybiphenyl + sulfite

-

Paenibacillus sp. A11-2

ir

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

Substrate Product ID

2-(2'-hydroxyphenyl)benzenesulfinate + H2O

-

649406

Paenibacillus sp.

2-hydroxybiphenyl + sulfite

-

649406

Paenibacillus sp.

ir

2-(2'-hydroxyphenyl)benzenesulfinate + H2O

final step of the desulfurozation of dibenzothiophene

649406

Paenibacillus sp.

2-hydroxybiphenyl + sulfite

-

649406

Paenibacillus sp.

ir

2-(2'-hydroxyphenyl)benzenesulfinate + H2O

-

649406

Paenibacillus sp. A11-2

2-hydroxybiphenyl + sulfite

-

649406

Paenibacillus sp. A11-2

ir

2-(2'-hydroxyphenyl)benzenesulfinate + H2O

final step of the desulfurozation of dibenzothiophene

649406

Paenibacillus sp. A11-2

2-hydroxybiphenyl + sulfite

-

649406

Paenibacillus sp. A11-2

ir

2-(2'-hydroxyphenyl)ethene-1-sulfinate + H2O

-

649406

Paenibacillus sp.

o-hydroxystyrene + sulfite

-

649406

Paenibacillus sp.

?

2-(2'-hydroxyphenyl)ethene-1-sulfinate + H2O

-

649406

Paenibacillus sp. A11-2

o-hydroxystyrene + sulfite

-

649406

Paenibacillus sp. A11-2

?

Activating Compound

Commentary

Organism

Structure

Mg2+

1.23fold activation at 1 mM

Paenibacillus sp.

Inhibitors

Commentary

Organism

Structure

2-Hydroxybiphenyl

30% inhibition at 5 mM, the OH-group is important for the inhibitory effect

Paenibacillus sp.

Cu2+

67% inhibition at 1 mM

Paenibacillus sp.

DTNB

86% inhibition at 0.1 mM

Paenibacillus sp.

Fe2+

44% inhibition at 1 mM

Paenibacillus sp.

iodoacetic acid

95% inhibition at 1 mM

Paenibacillus sp.

Mn2+

71% inhibition at 1 mM

Paenibacillus sp.

additional information

no inhibition by 1,10-phenanthroline and EDTA, no inhibition by Co2+, Ca2+, and Mg2+, no inhibition by biphenyl, 2-methylbiphenyl, and 2-methoxybiphenyl

Paenibacillus sp.

N-ethylmaleimide

complete inhibition at 1 mM

Paenibacillus sp.

Ni2+

24% inhibition at 1 mM

Paenibacillus sp.

o-hydroxystyrene

product inhibition, 80% inhibition at 5 mM, the OH-group is important for the inhibitory effect

Paenibacillus sp.

p-chloromercuribenzoate

92% inhibition at 0.1 mM

Paenibacillus sp.

Zn2+

98% inhibition at 1 mM

Paenibacillus sp.

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.33

-

2-(2-hydroxyphenyl)benzenesulfinate

pH 7.0, 50°C

Paenibacillus sp.

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

2-(2'-hydroxyphenyl)benzenesulfinate + H2O

Paenibacillus sp.

final step of the desulfurozation of dibenzothiophene

2-hydroxybiphenyl + sulfite

-

Paenibacillus sp.

ir

2-(2'-hydroxyphenyl)benzenesulfinate + H2O

Paenibacillus sp. A11-2

final step of the desulfurozation of dibenzothiophene

2-hydroxybiphenyl + sulfite

-

Paenibacillus sp. A11-2

ir

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

ID

2-(2'-hydroxyphenyl)benzenesulfinate + H2O

-

649406

Paenibacillus sp.

2-hydroxybiphenyl + sulfite

-

649406

Paenibacillus sp.

ir

2-(2'-hydroxyphenyl)benzenesulfinate + H2O

final step of the desulfurozation of dibenzothiophene

649406

Paenibacillus sp.

2-hydroxybiphenyl + sulfite

-

649406

Paenibacillus sp.

ir

2-(2'-hydroxyphenyl)benzenesulfinate + H2O

-

649406

Paenibacillus sp. A11-2

2-hydroxybiphenyl + sulfite

-

649406

Paenibacillus sp. A11-2

ir

2-(2'-hydroxyphenyl)benzenesulfinate + H2O

final step of the desulfurozation of dibenzothiophene

649406

Paenibacillus sp. A11-2

2-hydroxybiphenyl + sulfite

-

649406

Paenibacillus sp. A11-2

ir

2-(2'-hydroxyphenyl)ethene-1-sulfinate + H2O

-

649406

Paenibacillus sp.

o-hydroxystyrene + sulfite

-

649406

Paenibacillus sp.

?

2-(2'-hydroxyphenyl)ethene-1-sulfinate + H2O

-

649406

Paenibacillus sp. A11-2

o-hydroxystyrene + sulfite

-

649406

Paenibacillus sp. A11-2

?

No.

1st author

Pub Med

title

organims

journal

volume

pages

year

Activating Compound

Application

Cloned(Commentary)

Crystallization (Commentary)

Engineering

General Stability

Inhibitors

KM Value [mM]

Localization

Metals/Ions

Molecular Weight [Da]

Natural Substrates/ Products (Substrates)

Organic Solvent Stability

Organism

Oxidation Stability

Posttranslational Modification

Purification (Commentary)

Reaction

Renatured (Commentary)

Source Tissue

Specific Activity [micromol/min/mg]

Storage Stability

Substrates and Products (Substrate)

Subunits

Synonyms

Temperature Optimum [°C]

Temperature Range [°C]

Temperature Stability [°C]

Turnover Number [1/s]

pH Optimum

pH Range

pH Stability

Cofactor

Ki Value [mM]

pI Value

IC50 Value

Activating Compound (protein specific)

Application (protein specific)

Cloned(Commentary) (protein specific)

Cofactor (protein specific)

Crystallization (Commentary) (protein specific)

Engineering (protein specific)

General Stability (protein specific)

IC50 Value (protein specific)

Inhibitors (protein specific)

Ki Value [mM] (protein specific)

KM Value [mM] (protein specific)

Localization (protein specific)

Metals/Ions (protein specific)

Molecular Weight [Da] (protein specific)

Natural Substrates/ Products (Substrates) (protein specific)

Organic Solvent Stability (protein specific)

Oxidation Stability (protein specific)

Posttranslational Modification (protein specific)

Purification (Commentary) (protein specific)

Renatured (Commentary) (protein specific)

Source Tissue (protein specific)

Specific Activity [micromol/min/mg] (protein specific)

Storage Stability (protein specific)

Substrates and Products (Substrate) (protein specific)

Subunits (protein specific)

Temperature Optimum [°C] (protein specific)

Temperature Range [°C] (protein specific)

Temperature Stability [°C] (protein specific)

Turnover Number [1/s] (protein specific)

pH Optimum (protein specific)

pH Range (protein specific)

pH Stability (protein specific)

pI Value (protein specific)

Expression

General Information

General Information (protein specific)

Expression (protein specific)

KCat/KM [mM/s]

KCat/KM [mM/s] (protein specific)

695786

Calzada

Analysis of dibenzothiophene d ...

Pseudomonas putida, Pseudomonas putida CECT 5279

Appl. Environ. Microbiol.

75

875-877

2009

-

2

1

-

-

-

-

-

-

-

-

2

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2

-

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1

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2

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2

1

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2

1

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-

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2

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-

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-

-

1

-

2

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

695760

Li

Genetic rearrangement strategy ...

Rhodococcus erythropolis

Appl. Environ. Microbiol.

74

971-976

2008

-

2

1

-

-

-

-

-

-

-

-

1

-

1

-

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2

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2

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2

1

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1

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-

-

2

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

683271

Ohshiro

Improvement of 2-hydroxybiphen ...

Rhodococcus erythropolis, Rhodococcus erythropolis IGTS8 / ATCC 53968

Biosci. Biotechnol. Biochem.

71

2815-2821

2007

-

-

1

-

7

-

-

4

-

-

-

-

-

3

-

-

-

-

-

-

8

-

2

-

-

-

-

2

-

-

-

-

-

-

-

-

-

-

1

-

-

7

-

-

-

-

4

-

-

-

-

-

-

-

-

-

-

8

-

2

-

-

-

2

-

-

-

-

-

-

-

-

-

-

-

669500

Lee

Crystal structure and desulfur ...

Rhodococcus erythropolis

J. Biol. Chem.

281

32534-32539

2006

-

-

-

1

3

-

-

-

-

-

-

-

-

2

-

-

-

-

-

-

-

-

1

-

2

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

3

-

-

-

-

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-

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-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

657474

Lee

Crystallization and preliminar ...

Rhodococcus sp., Rhodococcus sp. IGTS8

Acta Crystallogr. Sect. D

D60

1636-1638

2004

-

-

-

1

-

-

-

-

-

-

-

-

-

2

-

-

1

-

-

-

-

-

2

-

1

-

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-

-

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-

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-

1

-

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-

-

-

-

1

-

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-

-

2

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

649406

Konishi

2-(2'-Hydroxyphenyl)benzene su ...

Paenibacillus sp., Paenibacillus sp. A11-2

Appl. Microbiol. Biotechnol.

62

356-361

2003

1

-

1

-

1

-

12

1

-

-

2

2

-

6

-

-

1

-

-

-

1

-

6

1

2

1

-

2

-

1

-

-

1

-

-

-

1

-

1

1

-

1

-

-

12

-

1

-

-

2

2

-

-

-

1

-

-

1

-

6

1

1

-

2

-

1

-

-

-

-

-

-

-

-

-

649487

Watkins

Purification and characterizat ...

Rhodococcus erythropolis, Rhodococcus erythropolis IGTS8 / ATCC 53968

Arch. Biochem. Biophys.

415

14-23

2003

2

-

-

-

-

-

10

1

-

-

1

2

-

8

-

-

1

-

-

-

1

-

6

1

1

1

1

-

-

1

1

-

-

-

1

-

2

-

-

-

-

-

-

-

10

-

1

-

-

1

2

-

-

-

1

-

-

1

-

6

1

1

1

-

-

1

1

-

1

-

-

-

-

-

-

650387

Nakayama

A novel enzyme, 2'-hydroxybiph ...

Rhodococcus erythropolis, Rhodococcus erythropolis KA2-5-1

Biochim. Biophys. Acta

1598

122-130

2002

1

-

1

-

1

-

10

2

-

1

2

2

-

5

-

-

1

1

-

-

2

-

6

1

2

1

-

2

-

1

-

-

1

3

-

-

1

-

1

1

-

1

-

-

10

3

2

-

1

2

2

-

-

-

1

-

-

2

-

6

1

1

-

2

-

1

-

-

-

-

-

-

-

-

-

208354

Oldfield

Elucidation of the metabolic p ...

Rhodococcus rhodochrous, Rhodococcus rhodochrous IGTS8 / ATCC 53968

Microbiology

143

2961-2973

1997

-

-

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-

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-

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2

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2

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1

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2

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-