Data extracted from this reference:
Activating Compound
Mg2+
1.23fold activation at 1 mM
Paenibacillus sp.
Cloned(Commentary)
gene tdsB, DNA sequence determination, overexpression in Escherichia coli BL21 (DE3)
Paenibacillus sp.
Engineering
C17S
site-directed mutagenesis of the only cysteine residue results in an completely inactive mutant
Paenibacillus sp.
Inhibitors
2-Hydroxybiphenyl
30% inhibition at 5 mM, the OH-group is important for the inhibitory effect
Paenibacillus sp.
Cu2+
67% inhibition at 1 mM
Paenibacillus sp.
DTNB
86% inhibition at 0.1 mM
Paenibacillus sp.
Fe2+
44% inhibition at 1 mM
Paenibacillus sp.
iodoacetic acid
95% inhibition at 1 mM
Paenibacillus sp.
Mn2+
71% inhibition at 1 mM
Paenibacillus sp.
additional information
no inhibition by 1,10-phenanthroline and EDTA, no inhibition by Co2+, Ca2+, and Mg2+, no inhibition by biphenyl, 2-methylbiphenyl, and 2-methoxybiphenyl
Paenibacillus sp.
N-ethylmaleimide
complete inhibition at 1 mM
Paenibacillus sp.
Ni2+
24% inhibition at 1 mM
Paenibacillus sp.
o-hydroxystyrene
product inhibition, 80% inhibition at 5 mM, the OH-group is important for the inhibitory effect
Paenibacillus sp.
p-chloromercuribenzoate
92% inhibition at 0.1 mM
Paenibacillus sp.
Zn2+
98% inhibition at 1 mM
Paenibacillus sp.
KM Value [mM]
0.33
2-(2-hydroxyphenyl)benzenesulfinate
pH 7.0, 50°C
Paenibacillus sp.
Molecular Weight [Da]
31000
gel filtration
Paenibacillus sp.
39000
1 * 39000, SDS-PAGE
Paenibacillus sp.
Natural Substrates/ Products (Substrates)
2-(2'-hydroxyphenyl)benzenesulfinate + H2O
Paenibacillus sp.
final step of the desulfurozation of dibenzothiophene
2-hydroxybiphenyl + sulfite
Paenibacillus sp.
ir
2-(2'-hydroxyphenyl)benzenesulfinate + H2O
Paenibacillus sp. A11-2
final step of the desulfurozation of dibenzothiophene
2-hydroxybiphenyl + sulfite
Paenibacillus sp. A11-2
ir
Organism
Purification (Commentary)
native enzyme from strain A11-2 63fold
Paenibacillus sp.
Specific Activity [micromol/min/mg]
0.139
purified enzyme
Paenibacillus sp.
Substrates and Products (Substrate)
2-(2'-hydroxyphenyl)benzenesulfinate + H2O
649406
Paenibacillus sp.
2-hydroxybiphenyl + sulfite
649406
Paenibacillus sp.
ir
2-(2'-hydroxyphenyl)benzenesulfinate + H2O
final step of the desulfurozation of dibenzothiophene
649406
Paenibacillus sp.
2-hydroxybiphenyl + sulfite
649406
Paenibacillus sp.
ir
2-(2'-hydroxyphenyl)benzenesulfinate + H2O
649406
Paenibacillus sp. A11-2
2-hydroxybiphenyl + sulfite
649406
Paenibacillus sp. A11-2
ir
2-(2'-hydroxyphenyl)benzenesulfinate + H2O
final step of the desulfurozation of dibenzothiophene
649406
Paenibacillus sp. A11-2
2-hydroxybiphenyl + sulfite
649406
Paenibacillus sp. A11-2
ir
2-(2'-hydroxyphenyl)ethene-1-sulfinate + H2O
649406
Paenibacillus sp.
o-hydroxystyrene + sulfite
649406
Paenibacillus sp.
?
2-(2'-hydroxyphenyl)ethene-1-sulfinate + H2O
649406
Paenibacillus sp. A11-2
o-hydroxystyrene + sulfite
649406
Paenibacillus sp. A11-2
?
Subunits
monomer
1 * 39000, SDS-PAGE
Paenibacillus sp.
Synonyms
2-(2'-hydroxyphenyl)benzene sulfinate desulfinase
Paenibacillus sp.
Temperature Optimum [°C]
Temperature Stability [°C]
55
30 min, stable up to
Paenibacillus sp.
60
rapid loss of activity
Paenibacillus sp.
pH Optimum
Cofactor
additional information
no cofactors required
Paenibacillus sp.
Activating Compound (protein specific)
Mg2+
1.23fold activation at 1 mM
Paenibacillus sp.
Cloned(Commentary) (protein specific)
gene tdsB, DNA sequence determination, overexpression in Escherichia coli BL21 (DE3)
Paenibacillus sp.
Cofactor (protein specific)
additional information
no cofactors required
Paenibacillus sp.
Engineering (protein specific)
C17S
site-directed mutagenesis of the only cysteine residue results in an completely inactive mutant
Paenibacillus sp.
Inhibitors (protein specific)
2-Hydroxybiphenyl
30% inhibition at 5 mM, the OH-group is important for the inhibitory effect
Paenibacillus sp.
Cu2+
67% inhibition at 1 mM
Paenibacillus sp.
DTNB
86% inhibition at 0.1 mM
Paenibacillus sp.
Fe2+
44% inhibition at 1 mM
Paenibacillus sp.
iodoacetic acid
95% inhibition at 1 mM
Paenibacillus sp.
Mn2+
71% inhibition at 1 mM
Paenibacillus sp.
additional information
no inhibition by 1,10-phenanthroline and EDTA, no inhibition by Co2+, Ca2+, and Mg2+, no inhibition by biphenyl, 2-methylbiphenyl, and 2-methoxybiphenyl
Paenibacillus sp.
N-ethylmaleimide
complete inhibition at 1 mM
Paenibacillus sp.
Ni2+
24% inhibition at 1 mM
Paenibacillus sp.
o-hydroxystyrene
product inhibition, 80% inhibition at 5 mM, the OH-group is important for the inhibitory effect
Paenibacillus sp.
p-chloromercuribenzoate
92% inhibition at 0.1 mM
Paenibacillus sp.
Zn2+
98% inhibition at 1 mM
Paenibacillus sp.
KM Value [mM] (protein specific)
0.33
2-(2-hydroxyphenyl)benzenesulfinate
pH 7.0, 50°C
Paenibacillus sp.
Molecular Weight [Da] (protein specific)
31000
gel filtration
Paenibacillus sp.
39000
1 * 39000, SDS-PAGE
Paenibacillus sp.
Natural Substrates/ Products (Substrates) (protein specific)
2-(2'-hydroxyphenyl)benzenesulfinate + H2O
Paenibacillus sp.
final step of the desulfurozation of dibenzothiophene
2-hydroxybiphenyl + sulfite
Paenibacillus sp.
ir
2-(2'-hydroxyphenyl)benzenesulfinate + H2O
Paenibacillus sp. A11-2
final step of the desulfurozation of dibenzothiophene
2-hydroxybiphenyl + sulfite
Paenibacillus sp. A11-2
ir
Purification (Commentary) (protein specific)
native enzyme from strain A11-2 63fold
Paenibacillus sp.
Specific Activity [micromol/min/mg] (protein specific)
0.139
purified enzyme
Paenibacillus sp.
Substrates and Products (Substrate) (protein specific)
2-(2'-hydroxyphenyl)benzenesulfinate + H2O
649406
Paenibacillus sp.
2-hydroxybiphenyl + sulfite
649406
Paenibacillus sp.
ir
2-(2'-hydroxyphenyl)benzenesulfinate + H2O
final step of the desulfurozation of dibenzothiophene
649406
Paenibacillus sp.
2-hydroxybiphenyl + sulfite
649406
Paenibacillus sp.
ir
2-(2'-hydroxyphenyl)benzenesulfinate + H2O
649406
Paenibacillus sp. A11-2
2-hydroxybiphenyl + sulfite
649406
Paenibacillus sp. A11-2
ir
2-(2'-hydroxyphenyl)benzenesulfinate + H2O
final step of the desulfurozation of dibenzothiophene
649406
Paenibacillus sp. A11-2
2-hydroxybiphenyl + sulfite
649406
Paenibacillus sp. A11-2
ir
2-(2'-hydroxyphenyl)ethene-1-sulfinate + H2O
649406
Paenibacillus sp.
o-hydroxystyrene + sulfite
649406
Paenibacillus sp.
?
2-(2'-hydroxyphenyl)ethene-1-sulfinate + H2O
649406
Paenibacillus sp. A11-2
o-hydroxystyrene + sulfite
649406
Paenibacillus sp. A11-2
?
Subunits (protein specific)
monomer
1 * 39000, SDS-PAGE
Paenibacillus sp.
Temperature Optimum [°C] (protein specific)
Temperature Stability [°C] (protein specific)
55
30 min, stable up to
Paenibacillus sp.
60
rapid loss of activity
Paenibacillus sp.
pH Optimum (protein specific)
Other publictions for EC 3.13.1.3
695786
Calzada
Analysis of dibenzothiophene d ...
Pseudomonas putida, Pseudomonas putida CECT 5279
Appl. Environ. Microbiol.
75
875-877
2009
-
2
1
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
1
-
2
-
2
1
-
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
695760
Li
Genetic rearrangement strategy ...
Rhodococcus erythropolis
Appl. Environ. Microbiol.
74
971-976
2008
-
2
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
683271
Ohshiro
Improvement of 2-hydroxybiphen ...
Rhodococcus erythropolis, Rhodococcus erythropolis IGTS8 / ATCC 53968
Biosci. Biotechnol. Biochem.
71
2815-2821
2007
-
-
1
-
7
-
-
4
-
-
-
-
-
3
-
-
-
-
-
-
8
-
2
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
-
-
7
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
8
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
669500
Lee
Crystal structure and desulfur ...
Rhodococcus erythropolis
J. Biol. Chem.
281
32534-32539
2006
-
-
-
1
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
657474
Lee
Crystallization and preliminar ...
Rhodococcus sp., Rhodococcus sp. IGTS8
Acta Crystallogr. Sect. D
D60
1636-1638
2004
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
649406
Konishi
2-(2'-Hydroxyphenyl)benzene su ...
Paenibacillus sp., Paenibacillus sp. A11-2
Appl. Microbiol. Biotechnol.
62
356-361
2003
1
-
1
-
1
-
12
1
-
-
2
2
-
6
-
-
1
-
-
-
1
-
6
1
2
1
-
2
-
1
-
-
1
-
-
-
1
-
1
1
-
1
-
-
12
-
1
-
-
2
2
-
-
-
1
-
-
1
-
6
1
1
-
2
-
1
-
-
-
-
-
-
-
-
-
649487
Watkins
Purification and characterizat ...
Rhodococcus erythropolis, Rhodococcus erythropolis IGTS8 / ATCC 53968
Arch. Biochem. Biophys.
415
14-23
2003
2
-
-
-
-
-
10
1
-
-
1
2
-
8
-
-
1
-
-
-
1
-
6
1
1
1
1
-
-
1
1
-
-
-
1
-
2
-
-
-
-
-
-
-
10
-
1
-
-
1
2
-
-
-
1
-
-
1
-
6
1
1
1
-
-
1
1
-
1
-
-
-
-
-
-
650387
Nakayama
A novel enzyme, 2'-hydroxybiph ...
Rhodococcus erythropolis, Rhodococcus erythropolis KA2-5-1
Biochim. Biophys. Acta
1598
122-130
2002
1
-
1
-
1
-
10
2
-
1
2
2
-
5
-
-
1
1
-
-
2
-
6
1
2
1
-
2
-
1
-
-
1
3
-
-
1
-
1
1
-
1
-
-
10
3
2
-
1
2
2
-
-
-
1
-
-
2
-
6
1
1
-
2
-
1
-
-
-
-
-
-
-
-
-
208354
Oldfield
Elucidation of the metabolic p ...
Rhodococcus rhodochrous, Rhodococcus rhodochrous IGTS8 / ATCC 53968
Microbiology
143
2961-2973
1997
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-