BRENDA - Enzyme Database show
show all sequences of 3.13.1.3

2-(2'-Hydroxyphenyl)benzene sulfinate desulfinase from the thermophilic desulfurizing bacterium Paenibacillus sp. strain A11-2: purification and characterization

Konishi, J.; Maruhashi, K.; Appl. Microbiol. Biotechnol. 62, 356-361 (2003)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
Mg2+
1.23fold activation at 1 mM
Paenibacillus sp.
Cloned(Commentary)
Commentary
Organism
gene tdsB, DNA sequence determination, overexpression in Escherichia coli BL21 (DE3)
Paenibacillus sp.
Engineering
Amino acid exchange
Commentary
Organism
C17S
site-directed mutagenesis of the only cysteine residue results in an completely inactive mutant
Paenibacillus sp.
Inhibitors
Inhibitors
Commentary
Organism
Structure
2-Hydroxybiphenyl
30% inhibition at 5 mM, the OH-group is important for the inhibitory effect
Paenibacillus sp.
Cu2+
67% inhibition at 1 mM
Paenibacillus sp.
DTNB
86% inhibition at 0.1 mM
Paenibacillus sp.
Fe2+
44% inhibition at 1 mM
Paenibacillus sp.
iodoacetic acid
95% inhibition at 1 mM
Paenibacillus sp.
Mn2+
71% inhibition at 1 mM
Paenibacillus sp.
additional information
no inhibition by 1,10-phenanthroline and EDTA, no inhibition by Co2+, Ca2+, and Mg2+, no inhibition by biphenyl, 2-methylbiphenyl, and 2-methoxybiphenyl
Paenibacillus sp.
N-ethylmaleimide
complete inhibition at 1 mM
Paenibacillus sp.
Ni2+
24% inhibition at 1 mM
Paenibacillus sp.
o-hydroxystyrene
product inhibition, 80% inhibition at 5 mM, the OH-group is important for the inhibitory effect
Paenibacillus sp.
p-chloromercuribenzoate
92% inhibition at 0.1 mM
Paenibacillus sp.
Zn2+
98% inhibition at 1 mM
Paenibacillus sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.33
-
2-(2-hydroxyphenyl)benzenesulfinate
pH 7.0, 50°C
Paenibacillus sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
31000
-
gel filtration
Paenibacillus sp.
39000
-
1 * 39000, SDS-PAGE
Paenibacillus sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2-(2'-hydroxyphenyl)benzenesulfinate + H2O
Paenibacillus sp.
final step of the desulfurozation of dibenzothiophene
2-hydroxybiphenyl + sulfite
-
Paenibacillus sp.
ir
2-(2'-hydroxyphenyl)benzenesulfinate + H2O
Paenibacillus sp. A11-2
final step of the desulfurozation of dibenzothiophene
2-hydroxybiphenyl + sulfite
-
Paenibacillus sp. A11-2
ir
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Paenibacillus sp.
-
-
-
Paenibacillus sp. A11-2
-
-
-
Purification (Commentary)
Commentary
Organism
native enzyme from strain A11-2 63fold
Paenibacillus sp.
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.139
-
purified enzyme
Paenibacillus sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-(2'-hydroxyphenyl)benzenesulfinate + H2O
-
649406
Paenibacillus sp.
2-hydroxybiphenyl + sulfite
-
649406
Paenibacillus sp.
ir
2-(2'-hydroxyphenyl)benzenesulfinate + H2O
final step of the desulfurozation of dibenzothiophene
649406
Paenibacillus sp.
2-hydroxybiphenyl + sulfite
-
649406
Paenibacillus sp.
ir
2-(2'-hydroxyphenyl)benzenesulfinate + H2O
-
649406
Paenibacillus sp. A11-2
2-hydroxybiphenyl + sulfite
-
649406
Paenibacillus sp. A11-2
ir
2-(2'-hydroxyphenyl)benzenesulfinate + H2O
final step of the desulfurozation of dibenzothiophene
649406
Paenibacillus sp. A11-2
2-hydroxybiphenyl + sulfite
-
649406
Paenibacillus sp. A11-2
ir
2-(2'-hydroxyphenyl)ethene-1-sulfinate + H2O
-
649406
Paenibacillus sp.
o-hydroxystyrene + sulfite
-
649406
Paenibacillus sp.
?
2-(2'-hydroxyphenyl)ethene-1-sulfinate + H2O
-
649406
Paenibacillus sp. A11-2
o-hydroxystyrene + sulfite
-
649406
Paenibacillus sp. A11-2
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 39000, SDS-PAGE
Paenibacillus sp.
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
55
-
-
Paenibacillus sp.
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
55
-
30 min, stable up to
Paenibacillus sp.
60
-
rapid loss of activity
Paenibacillus sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Paenibacillus sp.
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
no cofactors required
Paenibacillus sp.
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
Mg2+
1.23fold activation at 1 mM
Paenibacillus sp.
Cloned(Commentary) (protein specific)
Commentary
Organism
gene tdsB, DNA sequence determination, overexpression in Escherichia coli BL21 (DE3)
Paenibacillus sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
no cofactors required
Paenibacillus sp.
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
C17S
site-directed mutagenesis of the only cysteine residue results in an completely inactive mutant
Paenibacillus sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2-Hydroxybiphenyl
30% inhibition at 5 mM, the OH-group is important for the inhibitory effect
Paenibacillus sp.
Cu2+
67% inhibition at 1 mM
Paenibacillus sp.
DTNB
86% inhibition at 0.1 mM
Paenibacillus sp.
Fe2+
44% inhibition at 1 mM
Paenibacillus sp.
iodoacetic acid
95% inhibition at 1 mM
Paenibacillus sp.
Mn2+
71% inhibition at 1 mM
Paenibacillus sp.
additional information
no inhibition by 1,10-phenanthroline and EDTA, no inhibition by Co2+, Ca2+, and Mg2+, no inhibition by biphenyl, 2-methylbiphenyl, and 2-methoxybiphenyl
Paenibacillus sp.
N-ethylmaleimide
complete inhibition at 1 mM
Paenibacillus sp.
Ni2+
24% inhibition at 1 mM
Paenibacillus sp.
o-hydroxystyrene
product inhibition, 80% inhibition at 5 mM, the OH-group is important for the inhibitory effect
Paenibacillus sp.
p-chloromercuribenzoate
92% inhibition at 0.1 mM
Paenibacillus sp.
Zn2+
98% inhibition at 1 mM
Paenibacillus sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.33
-
2-(2-hydroxyphenyl)benzenesulfinate
pH 7.0, 50°C
Paenibacillus sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
31000
-
gel filtration
Paenibacillus sp.
39000
-
1 * 39000, SDS-PAGE
Paenibacillus sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2-(2'-hydroxyphenyl)benzenesulfinate + H2O
Paenibacillus sp.
final step of the desulfurozation of dibenzothiophene
2-hydroxybiphenyl + sulfite
-
Paenibacillus sp.
ir
2-(2'-hydroxyphenyl)benzenesulfinate + H2O
Paenibacillus sp. A11-2
final step of the desulfurozation of dibenzothiophene
2-hydroxybiphenyl + sulfite
-
Paenibacillus sp. A11-2
ir
Purification (Commentary) (protein specific)
Commentary
Organism
native enzyme from strain A11-2 63fold
Paenibacillus sp.
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.139
-
purified enzyme
Paenibacillus sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-(2'-hydroxyphenyl)benzenesulfinate + H2O
-
649406
Paenibacillus sp.
2-hydroxybiphenyl + sulfite
-
649406
Paenibacillus sp.
ir
2-(2'-hydroxyphenyl)benzenesulfinate + H2O
final step of the desulfurozation of dibenzothiophene
649406
Paenibacillus sp.
2-hydroxybiphenyl + sulfite
-
649406
Paenibacillus sp.
ir
2-(2'-hydroxyphenyl)benzenesulfinate + H2O
-
649406
Paenibacillus sp. A11-2
2-hydroxybiphenyl + sulfite
-
649406
Paenibacillus sp. A11-2
ir
2-(2'-hydroxyphenyl)benzenesulfinate + H2O
final step of the desulfurozation of dibenzothiophene
649406
Paenibacillus sp. A11-2
2-hydroxybiphenyl + sulfite
-
649406
Paenibacillus sp. A11-2
ir
2-(2'-hydroxyphenyl)ethene-1-sulfinate + H2O
-
649406
Paenibacillus sp.
o-hydroxystyrene + sulfite
-
649406
Paenibacillus sp.
?
2-(2'-hydroxyphenyl)ethene-1-sulfinate + H2O
-
649406
Paenibacillus sp. A11-2
o-hydroxystyrene + sulfite
-
649406
Paenibacillus sp. A11-2
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 39000, SDS-PAGE
Paenibacillus sp.
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
55
-
-
Paenibacillus sp.
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
55
-
30 min, stable up to
Paenibacillus sp.
60
-
rapid loss of activity
Paenibacillus sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Paenibacillus sp.
Other publictions for EC 3.13.1.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
695786
Calzada
Analysis of dibenzothiophene d ...
Pseudomonas putida, Pseudomonas putida CECT 5279
Appl. Environ. Microbiol.
75
875-877
2009
-
2
1
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
1
-
2
-
1
-
-
-
-
-
-
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2
1
-
-
-
-
-
-
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-
-
-
-
2
-
-
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-
1
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
695760
Li
Genetic rearrangement strategy ...
Rhodococcus erythropolis
Appl. Environ. Microbiol.
74
971-976
2008
-
2
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
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-
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-
-
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2
1
-
-
-
-
-
-
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-
1
-
-
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-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
683271
Ohshiro
Improvement of 2-hydroxybiphen ...
Rhodococcus erythropolis, Rhodococcus erythropolis IGTS8 / ATCC 53968
Biosci. Biotechnol. Biochem.
71
2815-2821
2007
-
-
1
-
7
-
-
4
-
-
-
-
-
3
-
-
-
-
-
-
8
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
-
-
7
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
8
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
669500
Lee
Crystal structure and desulfur ...
Rhodococcus erythropolis
J. Biol. Chem.
281
32534-32539
2006
-
-
-
1
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
657474
Lee
Crystallization and preliminar ...
Rhodococcus sp., Rhodococcus sp. IGTS8
Acta Crystallogr. Sect. D
D60
1636-1638
2004
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
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-
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-
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-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
649406
Konishi
2-(2'-Hydroxyphenyl)benzene su ...
Paenibacillus sp., Paenibacillus sp. A11-2
Appl. Microbiol. Biotechnol.
62
356-361
2003
1
-
1
-
1
-
12
1
-
-
2
2
-
5
-
-
1
-
-
-
1
-
6
1
1
-
2
-
1
-
-
1
-
-
-
1
-
1
1
-
1
-
-
12
-
1
-
-
2
2
-
-
-
1
-
-
1
-
6
1
1
-
2
-
1
-
-
-
-
-
-
-
-
-
649487
Watkins
Purification and characterizat ...
Rhodococcus erythropolis, Rhodococcus erythropolis IGTS8 / ATCC 53968
Arch. Biochem. Biophys.
415
14-23
2003
2
-
-
-
-
-
10
1
-
-
1
2
-
8
-
-
1
-
-
-
1
-
6
1
1
1
-
-
1
1
-
-
-
1
-
2
-
-
-
-
-
-
-
10
-
1
-
-
1
2
-
-
-
1
-
-
1
-
6
1
1
1
-
-
1
1
-
1
-
-
-
-
-
-
650387
Nakayama
A novel enzyme, 2'-hydroxybiph ...
Rhodococcus erythropolis, Rhodococcus erythropolis KA2-5-1
Biochim. Biophys. Acta
1598
122-130
2002
1
-
1
-
1
-
10
2
-
1
2
2
-
4
-
-
1
1
-
-
2
-
6
1
1
-
2
-
1
-
-
1
3
-
-
1
-
1
1
-
1
-
-
10
3
2
-
1
2
2
-
-
-
1
-
-
2
-
6
1
1
-
2
-
1
-
-
-
-
-
-
-
-
-
208354
Oldfield
Elucidation of the metabolic p ...
Rhodococcus rhodochrous, Rhodococcus sp. IGTS8 / ATCC 53968
Microbiology
143
2961-2973
1997
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
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-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
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-
-
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-
-
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