BRENDA - Enzyme Database show
show all sequences of 3.13.1.1

UDP-sulfoquinovose formation by Sulfolobus acidocaldarius

Zolghadr, B.; Gasselhuber, B.; Windwarder, M.; Pabst, M.; Kracher, D.; Kerndl, M.; Zayni, S.; Hofinger-Horvath, A.; Ludwig, R.; Haltrich, D.; Oostenbrink, C.; Obinger, C.; Kosma, P.; Messner, P.; Schäffer, C.; Extremophiles 19, 451-467 (2015)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
E147A
strongly impaired conversion of UDP-D-glucose to UDP-D-glucose-5,6-ene
Sulfolobus acidocaldarius
H95A
inactive mutant enzyme
Sulfolobus acidocaldarius
K186A
impaired conversion of UDP-D-glucose to UDP-D-glucose-5,6-ene
Sulfolobus acidocaldarius
M145A
inactive mutant enzyme
Sulfolobus acidocaldarius
R101A
inactive mutant enzyme
Sulfolobus acidocaldarius
T144A
mutant enzyme with fully retained activity, conversion of UDP-D-glucose to UDP-D-glucose-5,6-ene
Sulfolobus acidocaldarius
Y148A
strongly impaired conversion of UDP-D-glucose to UDP-D-glucose-5,6-ene
Sulfolobus acidocaldarius
Y182A
impaired conversion of UDP-D-glucose to UDP-D-glucose-5,6-ene
Sulfolobus acidocaldarius
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDP-alpha-D-glucose + sulfite
Sulfolobus acidocaldarius
the activated form of sulfoquinovose is required for its incorporation into glycoconjugates
UDP-alpha-D-sulfoquinovopyranose + H2O
-
-
?
UDP-alpha-D-glucose + sulfite
Sulfolobus acidocaldarius DSM 639
the activated form of sulfoquinovose is required for its incorporation into glycoconjugates
UDP-alpha-D-sulfoquinovopyranose + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Sulfolobus acidocaldarius
Q4JBJ3
-
-
Sulfolobus acidocaldarius DSM 639
Q4JBJ3
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UDP-alpha-D-glucose + sulfite
the activated form of sulfoquinovose is required for its incorporation into glycoconjugates
729697
Sulfolobus acidocaldarius
UDP-alpha-D-sulfoquinovopyranose + H2O
-
-
-
?
UDP-alpha-D-glucose + sulfite
final hydride transfer occurs to C-4 rather than C-6 of the enone form of UDP-4-dehydro-D-glucose, leaving this carbon accessible for the subsequent addition of sulfite
729697
Sulfolobus acidocaldarius
UDP-alpha-D-sulfoquinovopyranose + H2O
-
-
-
?
UDP-alpha-D-glucose + sulfite
the activated form of sulfoquinovose is required for its incorporation into glycoconjugates
729697
Sulfolobus acidocaldarius DSM 639
UDP-alpha-D-sulfoquinovopyranose + H2O
-
-
-
?
UDP-alpha-D-glucose + sulfite
final hydride transfer occurs to C-4 rather than C-6 of the enone form of UDP-4-dehydro-D-glucose, leaving this carbon accessible for the subsequent addition of sulfite
729697
Sulfolobus acidocaldarius DSM 639
UDP-alpha-D-sulfoquinovopyranose + H2O
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
70
-
-
Sulfolobus acidocaldarius
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
-
Sulfolobus acidocaldarius
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
E147A
strongly impaired conversion of UDP-D-glucose to UDP-D-glucose-5,6-ene
Sulfolobus acidocaldarius
H95A
inactive mutant enzyme
Sulfolobus acidocaldarius
K186A
impaired conversion of UDP-D-glucose to UDP-D-glucose-5,6-ene
Sulfolobus acidocaldarius
M145A
inactive mutant enzyme
Sulfolobus acidocaldarius
R101A
inactive mutant enzyme
Sulfolobus acidocaldarius
T144A
mutant enzyme with fully retained activity, conversion of UDP-D-glucose to UDP-D-glucose-5,6-ene
Sulfolobus acidocaldarius
Y148A
strongly impaired conversion of UDP-D-glucose to UDP-D-glucose-5,6-ene
Sulfolobus acidocaldarius
Y182A
impaired conversion of UDP-D-glucose to UDP-D-glucose-5,6-ene
Sulfolobus acidocaldarius
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDP-alpha-D-glucose + sulfite
Sulfolobus acidocaldarius
the activated form of sulfoquinovose is required for its incorporation into glycoconjugates
UDP-alpha-D-sulfoquinovopyranose + H2O
-
-
?
UDP-alpha-D-glucose + sulfite
Sulfolobus acidocaldarius DSM 639
the activated form of sulfoquinovose is required for its incorporation into glycoconjugates
UDP-alpha-D-sulfoquinovopyranose + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UDP-alpha-D-glucose + sulfite
the activated form of sulfoquinovose is required for its incorporation into glycoconjugates
729697
Sulfolobus acidocaldarius
UDP-alpha-D-sulfoquinovopyranose + H2O
-
-
-
?
UDP-alpha-D-glucose + sulfite
final hydride transfer occurs to C-4 rather than C-6 of the enone form of UDP-4-dehydro-D-glucose, leaving this carbon accessible for the subsequent addition of sulfite
729697
Sulfolobus acidocaldarius
UDP-alpha-D-sulfoquinovopyranose + H2O
-
-
-
?
UDP-alpha-D-glucose + sulfite
the activated form of sulfoquinovose is required for its incorporation into glycoconjugates
729697
Sulfolobus acidocaldarius DSM 639
UDP-alpha-D-sulfoquinovopyranose + H2O
-
-
-
?
UDP-alpha-D-glucose + sulfite
final hydride transfer occurs to C-4 rather than C-6 of the enone form of UDP-4-dehydro-D-glucose, leaving this carbon accessible for the subsequent addition of sulfite
729697
Sulfolobus acidocaldarius DSM 639
UDP-alpha-D-sulfoquinovopyranose + H2O
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
70
-
-
Sulfolobus acidocaldarius
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
-
Sulfolobus acidocaldarius
Other publictions for EC 3.13.1.1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
729697
Zolghadr
UDP-sulfoquinovose formation b ...
Sulfolobus acidocaldarius, Sulfolobus acidocaldarius DSM 639
Extremophiles
19
451-467
2015
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8
-
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2
-
4
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4
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1
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1
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8
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4
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1
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1
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726230
Brychkova
An essential role for tomato s ...
Solanum lycopersicum
Plant Physiol.
161
148-164
2013
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1
-
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-
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1
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1
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4
-
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1
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1
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1
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1
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1
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1
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2
2
2
2
-
-
720497
Meyer
Sulfoquinovose synthase - an i ...
Sulfolobus acidocaldarius, Sulfolobus acidocaldarius MW001
Mol. Microbiol.
82
1150-1163
2011
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-
1
-
1
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3
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5
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1
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4
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1
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1
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4
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2
2
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723680
Shimojima
Biosynthesis and functions of ...
Arabidopsis thaliana, Chlamydomonas reinhardtii, Rhodobacter sphaeroides, Spinacia oleracea
Prog. Lipid Res.
50
234-239
2011
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-
1
-
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1
3
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1
4
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8
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1
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4
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1
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4
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7
7
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695786
Calzada
Analysis of dibenzothiophene d ...
Pseudomonas putida, Pseudomonas putida CECT 5279
Appl. Environ. Microbiol.
75
875-877
2009
-
2
1
-
-
-
-
-
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2
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2
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1
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2
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1
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2
1
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1
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2
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1
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695760
Li
Genetic rearrangement strategy ...
Rhodococcus erythropolis
Appl. Environ. Microbiol.
74
971-976
2008
-
2
1
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-
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1
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1
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2
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1
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1
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2
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649481
Shimojima
Native uridine 5'-diphosphate- ...
Spinacia oleracea
Arch. Biochem. Biophys.
413
123-130
2003
-
-
1
-
-
-
-
1
1
-
2
1
-
3
-
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1
1
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1
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2
2
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1
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1
1
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2
1
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1
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1
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2
2
-
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-
-
-
-
-
-
-
-
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668388
Sato
Identification of a gene for U ...
Chlamydomonas reinhardtii
DNA Res.
10
229-237
2003
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1
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2
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393361
Sanda
Recombinant Arabidopsis SQD1 c ...
Arabidopsis sp., Arabidopsis thaliana
J. Biol. Chem.
276
3941-3946
2001
1
1
1
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2
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1
4
3
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1
1
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2
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1
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1
3
1
1
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2
1
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1
1
1
1
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2
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1
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4
3
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1
1
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1
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1
1
3
1
1
-
-
2
2
1
-
-
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-
-
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393362
Mulichak
Crystal structure of SQD1, an ...
Arabidopsis thaliana
Proc. Natl. Acad. Sci. USA
96
13097-13102
1999
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1
1
1
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3
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1
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1
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1
1
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393364
Essigmann
Prediction of the active-site ...
Arabidopsis thaliana
Arch. Biochem. Biophys.
369
30-41
1999
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1
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2
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393363
Essigmann
Phosphate availability affects ...
Arabidopsis thaliana
Proc. Natl. Acad. Sci. USA
95
1950-1955
1998
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1
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3
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