BRENDA - Enzyme Database show
show all sequences of 3.13.1.1

Crystal structure of SQD1, an enzyme involved in the biosynthesis of the plant sufolipid headgroup donor UDP-sulfoquinovose

Mulichak, A.M.; Theisen, M.J.; Essigmann, B.; Benning, C.; Garavito, R.M.; Proc. Natl. Acad. Sci. USA 96, 13097-13102 (1999)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli
Arabidopsis thaliana
Crystallization (Commentary)
Crystallization
Organism
sitting drop vapor diffusion results in bipyramidal crystals, C-2-symmetric homodimer, the protein has a bidomain structure, catalytic site is located in the cleft formed between the two lobes
Arabidopsis thaliana
Engineering
Amino acid exchange
Commentary
Organism
additional information
the function of different amino acids in reaction/binding process is analysed, Thr-145, Tyr-182 and Lys-186 are found to fulfill analogous mechanistic roles
Arabidopsis thaliana
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast
thylakoid
Arabidopsis thaliana
9507
-
membrane
photosynthetic
Arabidopsis thaliana
16020
-
thylakoid
-
Arabidopsis thaliana
9579
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDP-glucose + sulfite
Arabidopsis thaliana
sulfolipid biosynthesis
UDP-sulfoquinovose + H2O
-
Arabidopsis thaliana
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
O48917
-
-
Purification (Commentary)
Commentary
Organism
Ni2+-nitrilotriacetic acid column
Arabidopsis thaliana
Reaction
Reaction
Commentary
Organism
UDP-alpha-D-sulfoquinovopyranose + H2O = UDP-alpha-D-glucose + sulfite
contains characteristic conserved Y-XXX-K and glycine-rich sequence patterns; short-chain dehydrogenase/reductase family
Arabidopsis thaliana
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UDP-glucose + sulfite
-
393362
Arabidopsis thaliana
UDP-sulfoquinovose + H2O
-
393362
Arabidopsis thaliana
?
UDP-glucose + sulfite
sulfolipid biosynthesis
393362
Arabidopsis thaliana
UDP-sulfoquinovose + H2O
-
393362
Arabidopsis thaliana
?
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli
Arabidopsis thaliana
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Arabidopsis thaliana
Crystallization (Commentary) (protein specific)
Crystallization
Organism
sitting drop vapor diffusion results in bipyramidal crystals, C-2-symmetric homodimer, the protein has a bidomain structure, catalytic site is located in the cleft formed between the two lobes
Arabidopsis thaliana
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
the function of different amino acids in reaction/binding process is analysed, Thr-145, Tyr-182 and Lys-186 are found to fulfill analogous mechanistic roles
Arabidopsis thaliana
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast
thylakoid
Arabidopsis thaliana
9507
-
membrane
photosynthetic
Arabidopsis thaliana
16020
-
thylakoid
-
Arabidopsis thaliana
9579
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDP-glucose + sulfite
Arabidopsis thaliana
sulfolipid biosynthesis
UDP-sulfoquinovose + H2O
-
Arabidopsis thaliana
?
Purification (Commentary) (protein specific)
Commentary
Organism
Ni2+-nitrilotriacetic acid column
Arabidopsis thaliana
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UDP-glucose + sulfite
-
393362
Arabidopsis thaliana
UDP-sulfoquinovose + H2O
-
393362
Arabidopsis thaliana
?
UDP-glucose + sulfite
sulfolipid biosynthesis
393362
Arabidopsis thaliana
UDP-sulfoquinovose + H2O
-
393362
Arabidopsis thaliana
?
Other publictions for EC 3.13.1.1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
729697
Zolghadr
UDP-sulfoquinovose formation b ...
Sulfolobus acidocaldarius, Sulfolobus acidocaldarius DSM 639
Extremophiles
19
451-467
2015
-
-
-
-
8
-
-
-
-
-
-
2
-
4
-
-
-
-
-
-
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
726230
Brychkova
An essential role for tomato s ...
Solanum lycopersicum
Plant Physiol.
161
148-164
2013
-
-
1
-
-
-
-
-
1
-
-
1
-
4
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
2
2
2
2
-
-
720497
Meyer
Sulfoquinovose synthase - an i ...
Sulfolobus acidocaldarius, Sulfolobus acidocaldarius MW001
Mol. Microbiol.
82
1150-1163
2011
-
-
1
-
1
-
-
-
-
-
-
3
-
5
-
-
1
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
723680
Shimojima
Biosynthesis and functions of ...
Arabidopsis thaliana, Chlamydomonas reinhardtii, Rhodobacter sphaeroides, Spinacia oleracea
Prog. Lipid Res.
50
234-239
2011
-
-
1
-
-
-
-
1
3
-
1
4
-
8
-
-
1
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
3
-
1
4
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
7
7
-
-
-
695786
Calzada
Analysis of dibenzothiophene d ...
Pseudomonas putida, Pseudomonas putida CECT 5279
Appl. Environ. Microbiol.
75
875-877
2009
-
2
1
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
1
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
695760
Li
Genetic rearrangement strategy ...
Rhodococcus erythropolis
Appl. Environ. Microbiol.
74
971-976
2008
-
2
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
649481
Shimojima
Native uridine 5'-diphosphate- ...
Spinacia oleracea
Arch. Biochem. Biophys.
413
123-130
2003
-
-
1
-
-
-
-
1
1
-
2
1
-
3
-
-
1
1
-
1
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
1
-
2
1
-
-
-
1
-
1
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
668388
Sato
Identification of a gene for U ...
Chlamydomonas reinhardtii
DNA Res.
10
229-237
2003
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
393361
Sanda
Recombinant Arabidopsis SQD1 c ...
Arabidopsis sp., Arabidopsis thaliana
J. Biol. Chem.
276
3941-3946
2001
1
1
1
-
2
-
1
4
3
-
1
1
-
2
-
-
1
-
-
-
1
1
3
1
1
-
-
2
2
1
-
1
-
-
-
1
1
1
1
-
2
-
-
1
-
4
3
-
1
1
-
-
-
1
-
-
1
1
3
1
1
-
-
2
2
1
-
-
-
-
-
-
-
-
393362
Mulichak
Crystal structure of SQD1, an ...
Arabidopsis thaliana
Proc. Natl. Acad. Sci. USA
96
13097-13102
1999
-
-
1
1
1
-
-
-
3
-
-
1
-
1
-
-
1
1
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
1
-
-
-
-
-
3
-
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
393364
Essigmann
Prediction of the active-site ...
Arabidopsis thaliana
Arch. Biochem. Biophys.
369
30-41
1999
-
-
1
-
-
-
-
-
1
-
-
2
-
1
-
-
1
1
-
-
-
2
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
2
-
-
-
1
-
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
393363
Essigmann
Phosphate availability affects ...
Arabidopsis thaliana
Proc. Natl. Acad. Sci. USA
95
1950-1955
1998
-
-
1
-
-
-
-
-
3
-
1
1
-
1
-
-
1
1
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
1
1
-
-
-
1
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-