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Literature summary for 3.12.1.B1 extracted from
- The sole cysteine residue (Cys301) of tetrathionate hydrolase from Acidithiobacillus ferrooxidans does not play a role in enzyme activity (2014), Biosci. Biotechnol. Biochem., 78, 2030-2035.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, recombinant overexpression of wild-type and mutant enzymes in Escherichia coli strain BL21 Star(DE3) in inclusion bodies in an inactive form | Acidithiobacillus ferrooxidans |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C301A | site-directed mutagenesis, the enzyme activities measured in the cysteine-deficient and wild-type enzymes are the same | Acidithiobacillus ferrooxidans |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 93000 | - |
recombinant wild-type enzyme, gel filtration | Acidithiobacillus ferrooxidans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acidithiobacillus ferrooxidans | B7J3C9 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant inactive wild-type and mutant enzymes refolded from Escherichia coli strain BL21 Star(DE3) inclusion bodies | Acidithiobacillus ferrooxidans |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| refolding of recombinant inactive wild-type and mutant enzymes from Escherichia coli strain BL21 Star(DE3) inclusion bodies | Acidithiobacillus ferrooxidans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| tetrathionate + H2O | - |
Acidithiobacillus ferrooxidans | thiosulfate + sulfate + sulfur + 2 H+ | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | - |
Acidithiobacillus ferrooxidans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 4THase | - |
Acidithiobacillus ferrooxidans |
| Af-Tth | - |
Acidithiobacillus ferrooxidans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3 | - |
- |
Acidithiobacillus ferrooxidans |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the enzyme is a key enzyme in the dissimilatory sulfur oxidation pathway in this bacterium | Acidithiobacillus ferrooxidans |
| additional information | the sole cysteine residue, Cys301, of tetrathionate hydrolase from Acidithiobacillus ferrooxidans does neither play a role in enzyme activity nor in subunit assembly | Acidithiobacillus ferrooxidans |
| physiological function | the enzyme is key enzyme in the dissimilatory sulfur oxidation pathway and catalyzes tetrathionate hydrolysis to generate elemental sulfur, thiosulfate, and sulfate | Acidithiobacillus ferrooxidans |
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Release 2023.1 (January 2023)
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