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Literature summary for 3.12.1.B1 extracted from

  • De Jong, G.A.H.; Hazeu, W.; Bos, P.; Kuenen, J.G.
    Isolation of the tetrathionate hydrolase from Thiobacillus acidophilus (1997), Eur. J. Biochem., 243, 678-683.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
sulfate strong stimulation, maximal activity between 1 M and 2 M Acidiphilium acidophilum

Application

Application Comment Organism
analysis measurement of the enzyme activity during purification is based on the absorbance of the initial intermediates formed from tetrathionate in the ultraviolet region. Enzyme activity can also be measured by the scattering of insoluble sulfur in the visible region Acidiphilium acidophilum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
tetrathionate pH 3.0, 30°C, presence of 1 M ammonium sulfate Acidiphilium acidophilum

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Acidiphilium acidophilum
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
2 * 48000, SDS-PAGE Acidiphilium acidophilum
100000
-
gel filtration Acidiphilium acidophilum

Organism

Organism UniProt Comment Textmining
Acidiphilium acidophilum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Acidiphilium acidophilum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8
-
pH 3.0, 30°C Acidiphilium acidophilum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pentathionate + H2O
-
Acidiphilium acidophilum 2 sulfur + thiosulfate + sulfate + 2 H+
-
?
tetrathionate + H2O
-
Acidiphilium acidophilum sulfur + thiosulfate + sulfate + 2 H+
-
?

Subunits

Subunits Comment Organism
dimer 2 * 48000, SDS-PAGE Acidiphilium acidophilum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
Acidiphilium acidophilum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5
-
-
Acidiphilium acidophilum

pH Range

pH Minimum pH Maximum Comment Organism
1
-
no residual activity Acidiphilium acidophilum
7
-
no residual activity Acidiphilium acidophilum

Cofactor

Cofactor Comment Organism Structure
additional information presence of an organic cofactor. cofactor is not NAD+ or NADPH. No molybdenum is observed in the purified enzyme Acidiphilium acidophilum