BRENDA - Enzyme Database show
show all sequences of 3.12.1.B1

Isolation of the tetrathionate hydrolase from Thiobacillus acidophilus

De Jong, G.A.H.; Hazeu, W.; Bos, P.; Kuenen, J.G.; Eur. J. Biochem. 243, 678-683 (1997)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
sulfate
strong stimulation, maximal activity between 1 M and 2 M
Acidiphilium acidophilum
Application
Application
Commentary
Organism
analysis
measurement of the enzyme activity during purification is based on the absorbance of the initial intermediates formed from tetrathionate in the ultraviolet region. Enzyme activity can also be measured by the scattering of insoluble sulfur in the visible region
Acidiphilium acidophilum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.3
-
tetrathionate
pH 3.0, 30°C, presence of 1 M ammonium sulfate
Acidiphilium acidophilum
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
-
Acidiphilium acidophilum
-
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
48000
-
2 * 48000, SDS-PAGE
Acidiphilium acidophilum
100000
-
gel filtration
Acidiphilium acidophilum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Acidiphilium acidophilum
-
-
-
Purification (Commentary)
Commentary
Organism
-
Acidiphilium acidophilum
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
8
-
pH 3.0, 30°C
Acidiphilium acidophilum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
pentathionate + H2O
-
724902
Acidiphilium acidophilum
2 sulfur + thiosulfate + sulfate + 2 H+
-
-
-
?
tetrathionate + H2O
-
724902
Acidiphilium acidophilum
sulfur + thiosulfate + sulfate + 2 H+
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 48000, SDS-PAGE
Acidiphilium acidophilum
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
65
-
-
Acidiphilium acidophilum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.5
-
-
Acidiphilium acidophilum
pH Range
pH Minimum
pH Maximum
Commentary
Organism
1
-
no residual activity
Acidiphilium acidophilum
7
-
no residual activity
Acidiphilium acidophilum
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
presence of an organic cofactor. cofactor is not NAD+ or NADPH. No molybdenum is observed in the purified enzyme
Acidiphilium acidophilum
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
sulfate
strong stimulation, maximal activity between 1 M and 2 M
Acidiphilium acidophilum
Application (protein specific)
Application
Commentary
Organism
analysis
measurement of the enzyme activity during purification is based on the absorbance of the initial intermediates formed from tetrathionate in the ultraviolet region. Enzyme activity can also be measured by the scattering of insoluble sulfur in the visible region
Acidiphilium acidophilum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
presence of an organic cofactor. cofactor is not NAD+ or NADPH. No molybdenum is observed in the purified enzyme
Acidiphilium acidophilum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.3
-
tetrathionate
pH 3.0, 30°C, presence of 1 M ammonium sulfate
Acidiphilium acidophilum
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
-
Acidiphilium acidophilum
-
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
48000
-
2 * 48000, SDS-PAGE
Acidiphilium acidophilum
100000
-
gel filtration
Acidiphilium acidophilum
Purification (Commentary) (protein specific)
Commentary
Organism
-
Acidiphilium acidophilum
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
8
-
pH 3.0, 30°C
Acidiphilium acidophilum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
pentathionate + H2O
-
724902
Acidiphilium acidophilum
2 sulfur + thiosulfate + sulfate + 2 H+
-
-
-
?
tetrathionate + H2O
-
724902
Acidiphilium acidophilum
sulfur + thiosulfate + sulfate + 2 H+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 48000, SDS-PAGE
Acidiphilium acidophilum
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
65
-
-
Acidiphilium acidophilum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.5
-
-
Acidiphilium acidophilum
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
1
-
no residual activity
Acidiphilium acidophilum
7
-
no residual activity
Acidiphilium acidophilum
Other publictions for EC 3.12.1.B1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
733533
Kanao
The sole cysteine residue (Cys ...
Acidithiobacillus ferrooxidans
Biosci. Biotechnol. Biochem.
78
2030-2035
2014
-
-
1
-
1
-
-
-
-
-
1
-
-
2
-
-
1
-
1
-
-
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
1
1
-
-
-
1
1
-
-
-
-
1
-
-
-
-
3
3
-
-
-
734092
Yu
Construction and characterizat ...
Acidithiobacillus ferrooxidans
J. Bacteriol.
196
2255-2264
2014
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
723879
Kanao
Crystallization and preliminar ...
Acidithiobacillus ferrooxidans
Acta Crystallogr. Sect. F
69
692-694
2013
-
-
1
1
-
-
-
-
-
-
-
-
-
3
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
724016
Krupovic
Archaeal tetrathionate hydrola ...
Acidianus hospitalis, Acidianus hospitalis W1, Acidianus hospitalis YS8
Appl. Environ. Microbiol.
78
5463-5465
2012
-
-
-
1
-
-
-
-
2
-
1
-
-
5
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
725076
Protze
An extracellular tetrathionate ...
Acidianus ambivalens
Front. Microbiol.
2
68
2011
-
-
-
-
-
-
-
1
1
-
3
-
-
5
-
1
1
-
-
-
1
-
1
2
1
2
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
3
-
-
-
1
1
-
-
1
-
1
2
1
2
-
-
1
1
-
-
-
-
-
-
-
-
725078
Beard
Growth of Acidithiobacillus fe ...
Acidithiobacillus ferrooxidans
Front. Microbiol.
2
79
2011
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
725036
Kanao
Recombinant tetrathionate hydr ...
Acidithiobacillus ferrooxidans
FEMS Microbiol. Lett.
309
43-47
2010
-
-
1
-
-
-
-
-
1
-
-
-
-
3
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
724581
Sugio
Ferrous iron production mediat ...
Acidithiobacillus ferrooxidans
Biosci. Biotechnol. Biochem.
73
1381-1386
2009
-
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
1
-
-
-
-
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
724906
Bugaytsova
Localization, purification and ...
Acidithiobacillus caldus, Acidithiobacillus caldus DSM 8584
Eur. J. Biochem.
271
272-280
2004
1
-
-
-
-
-
-
-
1
4
2
-
-
4
-
-
-
-
-
-
1
-
2
1
1
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
1
4
2
-
-
-
-
-
-
-
1
-
2
1
1
-
-
-
1
-
-
1
-
-
-
-
-
-
724902
De Jong
Isolation of the tetrathionate ...
Acidiphilium acidophilum
Eur. J. Biochem.
243
678-683
1997
1
1
-
-
-
-
-
1
1
-
2
-
-
2
-
-
1
-
-
-
1
-
2
1
1
-
-
-
1
2
-
1
-
-
-
1
1
-
1
-
-
-
-
-
-
1
1
-
2
-
-
-
-
1
-
-
1
-
2
1
1
-
-
-
1
2
-
-
-
-
-
-
-
-
725891
De Jong
-
Polythionate degradation by te ...
Acidithiobacillus ferrooxidans
Microbiology
143
499-504
1997
-
1
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
725029
Meulenberg
-
Metabolism of tetrathionate in ...
Acidiphilium acidophilum
FEMS Microbiol. Lett.
112
167-172
1993
1
-
-
-
-
-
3
1
1
-
-
-
-
1
-
-
1
-
-
-
-
2
2
-
1
-
2
-
1
2
-
-
-
-
-
1
-
-
-
-
-
-
-
3
-
1
1
-
-
-
-
-
-
1
-
-
-
2
2
-
1
-
2
-
1
2
-
-
-
-
-
-
-
-