Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli | Bacillus cereus |
Crystallization (Comment) | Organism |
---|---|
10 mg/ml wild-type and mutant D12A enzymes complexed with Mg2+ only or with Mg2+ and substrate, in 1 mM HEPES, 10 mM MgCl2, 0.1 mM DTT, pH 7.5, 4°C, hanging drop vapour diffusion method, equal volume of protein and reservoir solution, the latter containing 30% PEG 4000, 100 mM Tris-HCl, pH 7.4, 100 mM MgCl2, 1 week, against the reservoir well solution additionally with 20% glycerol before data collection, X-ray diffraction structure determination and analysis at 2.3-2.55 A | Bacillus cereus |
Protein Variants | Comment | Organism |
---|---|---|
D12A | site-directed mutagenesis, catalytically inactive mutant | Bacillus cereus |
D186A | site-directed mutagenesis, highly reduced activity | Bacillus cereus |
D186A/D190A | site-directed mutagenesis, inactive mutant | Bacillus cereus |
D186E | site-directed mutagenesis, very highly reduced activity | Bacillus cereus |
D190A | site-directed mutagenesis, reduced activity | Bacillus cereus |
G185D/D190G | site-directed mutagenesis, reduced activity | Bacillus cereus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics, activity of mutant D186A is too low to measure Km accurately | Bacillus cereus | |
0.033 | - |
phosphonoacetaldehyde | wild-type enzyme, pH 7.5, 25°C | Bacillus cereus | |
0.054 | - |
phosphonoacetaldehyde | mutant G185D/D190G, pH 7.5, 25°C | Bacillus cereus | |
0.072 | - |
phosphonoacetaldehyde | mutant D12E, pH 7.5, 25°C | Bacillus cereus | |
0.52 | - |
phosphonoacetaldehyde | mutant D190A, pH 7.5, 25°C | Bacillus cereus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activates, required for catalysis, serves as a cofactor, binds via ligation to the loop 1 Asp12 carboxylate and Thr 14 backbone carbonyl and to the loop 4 Asp186carboxylate, the loop 4 Asp190 forms a hydrogen bond to the Mg(II) water ligand, Asp186 is essential while Asp190 simply enhances cofactor binding | Bacillus cereus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus cereus | O31156 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
phosphonoacetaldehyde + H2O = acetaldehyde + phosphate | active site structure | Bacillus cereus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphonoacetaldehyde + H2O | - |
Bacillus cereus | acetaldehyde + phosphate | - |
ir |
Synonyms | Comment | Organism |
---|---|---|
phosphonatase | - |
Bacillus cereus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assy at | Bacillus cereus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000084 | - |
phosphonoacetaldehyde | mutant D186A, pH 7.5, 25°C | Bacillus cereus | |
0.0012 | - |
phosphonoacetaldehyde | mutant D12E, pH 7.5, 25°C | Bacillus cereus | |
0.022 | - |
phosphonoacetaldehyde | mutant D190A, pH 7.5, 25°C | Bacillus cereus | |
1.7 | - |
phosphonoacetaldehyde | mutant G185D/D190G, pH 7.5, 25°C | Bacillus cereus | |
15 | - |
phosphonoacetaldehyde | wild-type enzyme, pH 7.5, 25°C | Bacillus cereus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Bacillus cereus |