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Literature summary for 3.11.1.1 extracted from

  • La Nauze, J.M.; Rosenberg, H.; Shaw, D.C.
    The enzyme cleavage of the carbon-phosphorous bond: purification and properties of phosphonatase (1970), Biochim. Biophys. Acta, 212, 332-350.
    View publication on PubMed
Search for more literature for 3.11.1.1

General Stability

General Stability Organism
inactivation in absence of Mg2+ is aggravated at higher pH or when either EDTA or Ca2+ is added Bacillus cereus
repeated freezing and thawing causes rapid loss of activity Bacillus cereus

Inhibitors

Inhibitors Comment Organism Structure
CN-
-
 Bacillus cereus
NaCl
-
 Bacillus cereus
phosphite
-
 Bacillus cereus
S2-
-
 Bacillus cereus
SO32-
-
 Bacillus cereus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
 phosphonoacetaldehyde
-
 Bacillus cereus

Metals/Ions

Metals/Ions Comment Organism Structure
copper contains 0.3 gatom per molecular weight of 75000 Da Bacillus cereus
Iron contains trace amounts of iron Bacillus cereus
Mg2+ required Bacillus cereus
Zinc contains 0.4 gatom per molecular weight of 75000 Da Bacillus cereus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68000
-
 nondenaturing PAGE Bacillus cereus
83000
-
 gel filtration Bacillus cereus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphonoacetylaldehyde + H2O Bacillus cereus second step in the pathway by which Bacillus cereus metabolizes 2-aminoethylphosphonic acid acetaldehyde + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus cereus
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
13.9
-
-
 Bacillus cereus

Storage Stability

Storage Stability Organism
-20°C, stable for several months Bacillus cereus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
p-nitrophenylphosphate + H2O hydrolyzed at considerable lower rate than phosphonoacetaldehyde Bacillus cereus p-nitrophenol + phosphate
-
 ?
phosphonoacetylaldehyde + H2O 2-phosphonoacetaldehyde Bacillus cereus acetaldehyde + phosphate
-
 ?
phosphonoacetylaldehyde + H2O second step in the pathway by which Bacillus cereus metabolizes 2-aminoethylphosphonic acid Bacillus cereus acetaldehyde + phosphate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 33000-37000, similar subunits, SDS-PAGE Bacillus cereus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Bacillus cereus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 55 30°C: about 50% of maximal activity, 55°C: about 45% of maximal activity Bacillus cereus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
 rapid loss loss of activity in absence of Mg2+ Bacillus cereus
45
-
 heat-labile, even in presence of 5 mM Mg2+ Bacillus cereus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
16.7
-
 2-phosphonoacetaldehyde
-
 Bacillus cereus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 9
-
 Bacillus cereus

pH Range

pH Minimum pH Maximum Comment Organism
6.5 10 pH 6.5: about 35% of maximal activity, pH 10.0: about 30% of maximal activity Bacillus cereus