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Literature summary for 3.10.1.1 extracted from
- Human liver sulphamate sulphohydrolase. Determination of native protein and subunit Mr values and influence of substrate aglycone structure on catalytic properties (1986), Biochem. J., 234, 83-92.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 1 mM decreases activity to 12% of control activity | Homo sapiens |  |
| SO42- | - |
Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56000 | - |
SDS-PAGE, reducing and non reducing | Homo sapiens |
| 100000 | - |
gel filtration, fractogel TSK | Homo sapiens |
| 122000 | - |
gel filtration, Sephacryl S-300 | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| heparan sulfate + H2O | Mammalia | - |
? + sulfate | - |
? | |
| heparan sulfate + H2O | Homo sapiens | - |
? + sulfate | - |
? | |
| heparin + H2O | Homo sapiens | - |
? + sulfate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Mammalia | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| apparent homogeneity | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | - |
Homo sapiens | - |
| liver | - |
Homo sapiens | - |
| placenta | - |
Homo sapiens | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4, sodium acetate, pH 5.0, 30% glycerol, 25 mM NaCl, 0.1 mM DTT, 0.3 mg/ml BSA, 3 month | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| heparan sulfate + H2O | - |
Mammalia | ? + sulfate | - |
? | |
| heparan sulfate + H2O | - |
Homo sapiens | ? + sulfate | - |
? | |
| heparan sulfate + H2O | - |
Homo sapiens | ? + sulfate | - |
ir | |
| heparin + H2O | - |
Homo sapiens | ? + sulfate | - |
? | |
| heparin + H2O | - |
Homo sapiens | ? + sulfate | - |
ir | |
| additional information | overview: effect of aglycone structure | Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 56000, SDS-PAGE | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Homo sapiens |
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