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Literature summary for 3.1.8.2 extracted from

  • Goldsmith, M.; Eckstein, S.; Ashani, Y.; Greisen, P.; Leader, H.; Sussman, J.L.; Aggarwal, N.; Ovchinnikov, S.; Tawfik, D.S.; Baker, D.; Thiermann, H.; Worek, F.
    Catalytic efficiencies of directly evolved phosphotriesterase variants with structurally different organophosphorus compounds in vitro (2016), Arch. Toxicol., 90, 2711-2724 .
    View publication on PubMed

Application

Application Comment Organism
additional information the organophosphorus-hydrolyzing enzymes, such as Brevundimonas diminuta phosphotriesterase (PTE), are used for development of alternative catalytic bioscavengers to treat organophosphorus agents, e.g. pesticides and warfare agents, intoxications of humans Brevundimonas diminuta

Cloned(Commentary)

Cloned (Comment) Organism
gene opd, recombinant expression of wild-type and mutant MBP-tagged enzymes in Escherichia coli strain BL21(DE3) Brevundimonas diminuta

Protein Variants

Protein Variants Comment Organism
additional information a PTE mutant dubbed C23 is engineered, exhibiting reversed stereoselectivity and high catalytic efficiency (kcat/KM) for the hydrolysis of the toxic enantiomers of nerve agents VX, CVX, and VR. The other mutants A53, IV-A1, IV-H3, B141, and RD1-G83 are less effective, activities and mutant-substrate docking, overview. The only exception is IV-A1 with amiton. Most variants are highly efficient when hydrolyzing N,N-diisopropyl substrates and are 2fold less efficient with N,N-diethyl substrates and 5fold less efficient with N,N-dimethyl substrates Brevundimonas diminuta

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information reaction kinetics of wild-type and mutant enzymes, overview Brevundimonas diminuta

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ required Brevundimonas diminuta

Organism

Organism UniProt Comment Textmining
Brevundimonas diminuta P0A434
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(RS)-propan-2-yl methylphosphonofluoridate + H2O i.e. sarin Brevundimonas diminuta propan-2-yl methylphosphonate + fluoride
-
?
3-[fluoro(methyl)phosphoryl]oxy-2,2-dimethylbutane + H2O i.e. soman Brevundimonas diminuta 3,3-dimethylbutan-2-yl methylphosphonate + fluoride
-
?
ethyl dimethylphosphoramidocyanidate + H2O i.e. tabun Brevundimonas diminuta ?
-
?
ethyl dimethylphosphoramidocyanidate + H2O i.e. tabun Brevundimonas diminuta ethyl hydrogen dimethylphosphoramidate + HCN
-
?
additional information the enzyme also exhibits activity of EC 3.1.8.1, aryldialkylphosphatase, as well as of EC 3.1.8.2, diisopropyl-fluorophosphatase. Enzyme-substrate docking study, overview Brevundimonas diminuta ?
-
?

Synonyms

Synonyms Comment Organism
More cf. EC 3.1.8.1 Brevundimonas diminuta
OP-hydrolyzing enzyme
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Brevundimonas diminuta
opd
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Brevundimonas diminuta
organophosphorus-hydrolyzing enzyme
-
Brevundimonas diminuta
PTES5
-
Brevundimonas diminuta

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
30
-
assay at Brevundimonas diminuta

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Brevundimonas diminuta