Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 cells | Loligo vulgaris |
Crystallization (Comment) | Organism |
---|---|
mutant enzymes are crystallized at room temperature by hanging drop vapor diffusion method, using 0.1 M Tris buffer pH 8.5, 2% tacsimate, 16% (w/v) PEG 3350 for mutant E37A/Y144A/R146A/T195M or 0.2 M KCl, 0.05 M HEPES buffer pH 7.5, 35% (w/v) pentaerythriol propoxylate for mutant E37D/Y144A/R16A/T195M | Loligo vulgaris |
Protein Variants | Comment | Organism |
---|---|---|
E37A/Y144A/R146A/T195M | the mutant shows increased turnover number and kcat/Km for diisopropyl fluorophosphate compared to the wild type enzyme | Loligo vulgaris |
E37D/Y144A/R16A/T195M | the mutant shows increased turnover number and kcat/Km for diisopropyl fluorophosphate compared to the wild type enzyme | Loligo vulgaris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.12 | - |
diisopropyl fluorophosphate | mutant enzyme E37A/Y144A/R146A/T195M | Loligo vulgaris | |
3.76 | - |
diisopropyl fluorophosphate | wild type enzyme | Loligo vulgaris | |
3.93 | - |
diisopropyl fluorophosphate | mutant enzyme E37D/Y144A/R16A/T195M | Loligo vulgaris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Loligo vulgaris | Q7SIG4 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Loligo vulgaris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-cyclosarin + H2O | - |
Loligo vulgaris | ? | - |
? | |
(R)-sarin + H2O | - |
Loligo vulgaris | ? | - |
? | |
(S)-cyclosarin + H2O | - |
Loligo vulgaris | ? | - |
? | |
(S)-sarin + H2O | - |
Loligo vulgaris | ? | - |
? | |
diisopropyl fluorophosphate + H2O | - |
Loligo vulgaris | diisopropyl phosphate + fluoride | - |
? | |
soman + H2O | - |
Loligo vulgaris | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DFPase | - |
Loligo vulgaris |
diisopropyl fluorophosphatase | - |
Loligo vulgaris |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
211 | - |
diisopropyl fluorophosphate | wild type enzyme | Loligo vulgaris | |
291 | - |
diisopropyl fluorophosphate | mutant enzyme E37A/Y144A/R146A/T195M | Loligo vulgaris | |
376 | - |
diisopropyl fluorophosphate | mutant enzyme E37D/Y144A/R16A/T195M | Loligo vulgaris |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
17 | - |
(S)-cyclosarin | wild type enzyme | Loligo vulgaris | |
24 | - |
(R)-sarin | mutant enzyme E37D/Y144A/R16A/T195M | Loligo vulgaris | |
24 | - |
(S)-cyclosarin | mutant enzyme E37D/Y144A/R16A/T195M | Loligo vulgaris | |
27 | - |
(R)-cyclosarin | mutant enzyme E37D/Y144A/R16A/T195M | Loligo vulgaris | |
42 | - |
(S)-sarin | wild type enzyme | Loligo vulgaris | |
45 | - |
(R)-sarin | mutant enzyme E37A/Y144A/R146A/T195M | Loligo vulgaris | |
47 | - |
(R)-sarin | wild type enzyme | Loligo vulgaris | |
56 | - |
diisopropyl fluorophosphate | wild type enzyme | Loligo vulgaris | |
71 | - |
(S)-sarin | mutant enzyme E37D/Y144A/R16A/T195M | Loligo vulgaris | |
96 | - |
diisopropyl fluorophosphate | mutant enzyme E37D/Y144A/R16A/T195M | Loligo vulgaris | |
130 | - |
(R)-cyclosarin | mutant enzyme E37A/Y144A/R146A/T195M | Loligo vulgaris | |
140 | - |
diisopropyl fluorophosphate | mutant enzyme E37A/Y144A/R146A/T195M | Loligo vulgaris | |
230 | - |
(S)-sarin | mutant enzyme E37A/Y144A/R146A/T195M | Loligo vulgaris | |
490 | - |
(S)-cyclosarin | mutant enzyme E37A/Y144A/R146A/T195M | Loligo vulgaris | |
720 | - |
(R)-cyclosarin | wild type enzyme | Loligo vulgaris |