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Literature summary for 3.1.8.2 extracted from

  • Choi, M.S.; Saxena, A.; Chilukuri, N.
    A strategy for the production of soluble human senescence marker protein-30 in Escherichia coli (2010), Biochem. Biophys. Res. Commun., 393, 509-513.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli DH5alpha cells Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diisopropyl fluorophosphate + H2O
-
Homo sapiens diisopropyl phosphate + fluoride
-
?

Synonyms

Synonyms Comment Organism
DFPase
-
Homo sapiens
diisopropyl fluorophosphatase
-
Homo sapiens
senescence marker protein-30
-
Homo sapiens
SMP30
-
Homo sapiens

Expression

Organism Comment Expression
Homo sapiens the coexpression of recombinant human SMP30 with GroES/GroEL/Tf at 15Ā°C, combined with the addition of a membrane fluidizer, increases osmolytes, and a two-step expression results in the highest enhancement of solubility and DFPase activity up