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Literature summary for 3.1.8.2 extracted from

  • Harvey, S.P.; Kolakowski, J.E.; Cheng, T.C.; Rastogi, V.K.; Reiff, L.P.; DeFrank, J.J.; Raushel, F.M.; Hill, C.
    Stereospecificity in the enzymatic hydrolysis of cyclosarin (GF) (2005), Enzyme Microb. Technol., 37, 547-555.
No PubMed abstract available
Search for more literature for 3.1.8.2

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Alteromonas sp.
expression in Escherichia coli Pseudoalteromonas haloplanktis

Organism

Organism UniProt Comment Textmining
Alteromonas sp.
-
 JD6.5
-
Alteromonas sp. JD6.5
-
 JD6.5
-
Pseudoalteromonas haloplanktis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Alteromonas sp.
-
 Pseudoalteromonas haloplanktis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4
-
 O-cyclohexyl methylphosphonofluoridate (-)isomer as substrate, pH 7.2, 1 mM MnCl2 Alteromonas sp.
2.7
-
 O-cyclohexyl methylphosphonofluoridate (-)isomer as substrate, pH 7.2, 1 mM MnCl2 Pseudoalteromonas haloplanktis
29.2
-
 O-cyclohexyl methylphosphonofluoridate (+)isomer as substrate, pH 7.2, 1 mM MnCl2 Alteromonas sp.
65.3
-
 O-cyclohexyl methylphosphonofluoridate (+)isomer as substrate, pH 7.2, 1 mM MnCl2 Pseudoalteromonas haloplanktis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cyclohexyl methyl fluorophosphate + H2O (+)isomer, pH 7.2, 1 mM MnCl2 Alteromonas sp. cyclohexyl methyl hydrogen phosphate + fluoride
-
 ?
cyclohexyl methyl fluorophosphate + H2O (+)isomer, pH 7.2, 1 mM MnCl2 Pseudoalteromonas haloplanktis cyclohexyl methyl hydrogen phosphate + fluoride
-
 ?
cyclohexyl methyl fluorophosphate + H2O (+)isomer, pH 7.2, 1 mM MnCl2 Alteromonas sp. JD6.5 cyclohexyl methyl hydrogen phosphate + fluoride
-
 ?
O-cyclohexyl methylphosphonofluoridate + H2O no reaction with the (-)isomer O-cyclohexyl methylphosphonofluoridate Alteromonas sp. O-cyclohexyl methylphosphonate + fluoride
-
 ?
O-cyclohexyl methylphosphonofluoridate + H2O no reaction with the (-)isomer O-cyclohexyl methylphosphonofluoridate Pseudoalteromonas haloplanktis O-cyclohexyl methylphosphonate + fluoride
-
 ?
O-cyclohexyl methylphosphonofluoridate + H2O no reaction with the (-)isomer O-cyclohexyl methylphosphonofluoridate Alteromonas sp. JD6.5 O-cyclohexyl methylphosphonate + fluoride
-
 ?

Synonyms

Synonyms Comment Organism
OPAA
-
 Alteromonas sp.
OPAA
-
 Pseudoalteromonas haloplanktis
organophosphorus acid anhydrolase
-
 Alteromonas sp.
organophosphorus acid anhydrolase
-
 Pseudoalteromonas haloplanktis