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Literature summary for 3.1.8.2 extracted from

  • Harvey, S.P.; Kolakowski, J.E.; Cheng, T.C.; Rastogi, V.K.; Reiff, L.P.; DeFrank, J.J.; Raushel, F.M.; Hill, C.
    Stereospecificity in the enzymatic hydrolysis of cyclosarin (GF) (2005), Enzyme Microb. Technol., 37, 547-555.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Alteromonas sp.
expression in Escherichia coli Pseudoalteromonas haloplanktis

Organism

Organism UniProt Comment Textmining
Alteromonas sp.
-
JD6.5
-
Alteromonas sp. JD6.5
-
JD6.5
-
Pseudoalteromonas haloplanktis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Alteromonas sp.
-
Pseudoalteromonas haloplanktis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [Āµmol/min/mg] Specific Activity Maximum [Āµmol/min/mg] Comment Organism
2.4
-
O-cyclohexyl methylphosphonofluoridate (-)isomer as substrate, pH 7.2, 1 mM MnCl2 Alteromonas sp.
2.7
-
O-cyclohexyl methylphosphonofluoridate (-)isomer as substrate, pH 7.2, 1 mM MnCl2 Pseudoalteromonas haloplanktis
29.2
-
O-cyclohexyl methylphosphonofluoridate (+)isomer as substrate, pH 7.2, 1 mM MnCl2 Alteromonas sp.
65.3
-
O-cyclohexyl methylphosphonofluoridate (+)isomer as substrate, pH 7.2, 1 mM MnCl2 Pseudoalteromonas haloplanktis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cyclohexyl methyl fluorophosphate + H2O (+)isomer, pH 7.2, 1 mM MnCl2 Alteromonas sp. cyclohexyl methyl hydrogen phosphate + fluoride
-
?
cyclohexyl methyl fluorophosphate + H2O (+)isomer, pH 7.2, 1 mM MnCl2 Pseudoalteromonas haloplanktis cyclohexyl methyl hydrogen phosphate + fluoride
-
?
cyclohexyl methyl fluorophosphate + H2O (+)isomer, pH 7.2, 1 mM MnCl2 Alteromonas sp. JD6.5 cyclohexyl methyl hydrogen phosphate + fluoride
-
?
O-cyclohexyl methylphosphonofluoridate + H2O no reaction with the (-)isomer O-cyclohexyl methylphosphonofluoridate Alteromonas sp. O-cyclohexyl methylphosphonate + fluoride
-
?
O-cyclohexyl methylphosphonofluoridate + H2O no reaction with the (-)isomer O-cyclohexyl methylphosphonofluoridate Pseudoalteromonas haloplanktis O-cyclohexyl methylphosphonate + fluoride
-
?
O-cyclohexyl methylphosphonofluoridate + H2O no reaction with the (-)isomer O-cyclohexyl methylphosphonofluoridate Alteromonas sp. JD6.5 O-cyclohexyl methylphosphonate + fluoride
-
?

Synonyms

Synonyms Comment Organism
OPAA
-
Alteromonas sp.
OPAA
-
Pseudoalteromonas haloplanktis
organophosphorus acid anhydrolase
-
Alteromonas sp.
organophosphorus acid anhydrolase
-
Pseudoalteromonas haloplanktis