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Literature summary for 3.1.8.2 extracted from

  • Hoskin, F.C.G.; Long, R.J.
    Purification of a DFP-hydrolyzing enzyme from squid head ganglion (1972), Arch. Biochem. Biophys., 150, 548-555.
    View publication on PubMed

General Stability

General Stability Organism
stable to prolonged dialysis procedures Doryteuthis pealeii

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ weak Doryteuthis pealeii
Cu2+
-
Doryteuthis pealeii
Hg2+ strong Doryteuthis pealeii
Mg2+ weak Doryteuthis pealeii
Mn2+ strong Doryteuthis pealeii
additional information no inhibition by K+ or NH4+ Doryteuthis pealeii
PCMB weak Doryteuthis pealeii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3
-
diisopropyl fluorophosphate pH 7.5, 22┬░C Doryteuthis pealeii
33
-
soman pH 7.5, 22┬░C Doryteuthis pealeii

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Spisula solidissima
-
-
soluble
-
Doryteuthis pealeii
-
-
soluble
-
Sepiidae
-
-
soluble
-
Aplysia sp.
-
-
soluble
-
Octopus sp.
-
-
soluble
-
Peltodoris nobilis
-
-

Organism

Organism UniProt Comment Textmining
Aplysia sp.
-
sea hare
-
Doryteuthis pealeii
-
squid
-
Octopus sp.
-
-
-
Peltodoris nobilis
-
Anisodoris
-
Sepiidae
-
Sepia
-
Spisula solidissima
-
surf clam
-

Purification (Commentary)

Purification (Comment) Organism
-
Doryteuthis pealeii

Source Tissue

Source Tissue Comment Organism Textmining
ganglion
-
Doryteuthis pealeii
-
ganglion
-
Sepiidae
-
ganglion
-
Aplysia sp.
-
ganglion
-
Octopus sp.
-
ganglion
-
Peltodoris nobilis
-
head ganglion Doryteuthis pealeii
-
head ganglion Sepiidae
-
head ganglion Aplysia sp.
-
head ganglion Octopus sp.
-
head ganglion Peltodoris nobilis
-
additional information tissue distribution Doryteuthis pealeii
-
pedal ganglion
-
Spisula solidissima
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
18.64
-
-
Doryteuthis pealeii

Storage Stability

Storage Stability Organism
frozen, less than 35% loss of activity within 6 months with repeated freeze-thawing cycles Doryteuthis pealeii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diisopropyl fluorophosphate + H2O i.e. DFP Spisula solidissima diisopropyl phosphate + fluoride
-
?
diisopropyl fluorophosphate + H2O i.e. DFP Doryteuthis pealeii diisopropyl phosphate + fluoride
-
?
diisopropyl fluorophosphate + H2O i.e. DFP Sepiidae diisopropyl phosphate + fluoride
-
?
diisopropyl fluorophosphate + H2O i.e. DFP Aplysia sp. diisopropyl phosphate + fluoride
-
?
diisopropyl fluorophosphate + H2O i.e. DFP Octopus sp. diisopropyl phosphate + fluoride
-
?
diisopropyl fluorophosphate + H2O i.e. DFP Peltodoris nobilis diisopropyl phosphate + fluoride
-
?
additional information not: 4-nitrophenylphosphate Doryteuthis pealeii ?
-
?
additional information not: ATP Doryteuthis pealeii ?
-
?
additional information not: methanesulfonyl fluoride, phenylmethanesulfonyl fluoride, monofluorophosphate, iso-octamethylpyrophosphoramide (i.e. ios-OMPA) Doryteuthis pealeii ?
-
?
soman + H2O
-
Doryteuthis pealeii ?
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
5 mM diisopropyl fluorophosphate Doryteuthis pealeii
8.5
-
1 mM diisopropyl fluorophosphate Doryteuthis pealeii