Application | Comment | Organism |
---|---|---|
medicine | h-PON1 is a strong candidate for the development of therapeutic intervention against many diseases due to its anti-inflammatory, anti-oxidative, anti-atherogenic, anti-diabetic, anti-microbial, and organophosphate (OP)-detoxifying properties in humans | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
recombinant overexpression of the C-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) in inclusion bodies | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
H115W/R192K | site-directed mutagenesis | Homo sapiens |
additional information | development of a recombinant production method for the enzyme in Escherichia coli, which can be used for the industrial scale production of rh-PON1 enzymes. The catalytic properties of the refolded enzymes are similar to their soluble counterparts | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | dependent on | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
diethyl-paraoxon + H2O | Homo sapiens | - |
diethyl phosphate + 4-nitrophenol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P27169 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged catalytically active paraoxwild-type and mutant enzymes refolded from inclusion bodies in Escherichia coli strain BL21(DE3) by anion exchange chromatography | Homo sapiens |
Renatured (Comment) | Organism |
---|---|
the inactive recombinant His6-tagged wild-type and mutant enzymes present in the inclusion bodies in Escherichia coli strain BL21(DE3) are refolded to their active form using in vitro refolding, best from refolding buffer containing 200 mM TAPS, pH 8.5, 1.0 M NDSB 201, 1 mM EDTA, 2.2 mM GSH, 0.22 mM GSSH, and 10 mM CaCl2, method optimization, overview. The catalytic properties of the refolded enzymes are similar to their soluble counterparts. The extent of refolding of rh-PON1 is more when 8 M urea is used as a chaotropic agent to denature the rh-PON1 present in inclusion bodies, low concentration of rh-PON1 protein (0.005 mg/ml) is used in the refolding reaction, and when the refolding reaction is incubated for 12 h at 25°C, but low concentration of protein in in vitro refolding is generally not economical for large-scale production of protein, thus 0.020 mg/ml protein concentration is selected for the refolding reaction | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
serum | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diethyl-paraoxon + H2O | - |
Homo sapiens | diethyl phosphate + 4-nitrophenol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
h-PON1 | - |
Homo sapiens |
human paraoxonase 1 | - |
Homo sapiens |
paraoxonase 1 | - |
Homo sapiens |
PON1 | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | the serum enzyme can hydrolyze (and inactivate) a wide range of substrates. It is a multifaceted enzyme and exhibit anti-inflammatory, anti-oxidative, anti-atherogenic, anti-diabetic, anti-microbial, and organophosphate (OP)-detoxifying properties | Homo sapiens |