Cloned (Comment) | Organism |
---|---|
gene opd, recombinant expression of wild-type and mutant enzymes, coexpression of CAW-tagged OPH and N-terminally His6-tagged TonB | Sphingobium fuliginis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | exbD and tonB genes of Sphingobium fuliginis are amplified wild-type strain ATCC 27551 from different plasmids, overview | Sphingobium fuliginis |
R91A/R96F | site-directed mutagenesis, substitution of the protein-interacting arginines, inactive mutant | Sphingobium fuliginis |
R91A/R96G | site-directed mutagenesis, substitution of the protein-interacting arginines | Sphingobium fuliginis |
R91F/R96F | site-directed mutagenesis, substitution of the protein-interacting arginines, inactive mutant | Sphingobium fuliginis |
R91F/R96G | site-directed mutagenesis, substitution of the protein-interacting arginines | Sphingobium fuliginis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | organophosphate hydrolase interacts with Ton components and is targeted to the membrane only in the presence of the ExbB/ExbD complex | Sphingobium fuliginis | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme contains a binuclear metal centre with two divalent cations | Sphingobium fuliginis | |
Zn2+ | required | Sphingobium fuliginis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethyl-parathion + H2O | Sphingobium fuliginis | - |
dimethyl thiophosphate + 4-nitrophenol | - |
? | |
additional information | Sphingobium fuliginis | organophosphate hydrolase interacts with Ton components and is targeted to the membrane only in the presence of the ExbB/ExbD complex | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sphingobium fuliginis | P0A433 | - |
- |
Purification (Comment) | Organism |
---|---|
co-purification of CAW-tagged OPH and N-terminally His6-tagged TonB | Sphingobium fuliginis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | cells are grown in a minimal medium containing dimethyl-parathion as sole source of phosphate reveal that proton motif force (PMF) is required for efficient uptake and utilization of dimethyl-parathion as sole source of phosphate, no growth is observed in cultures having PMF inhibitor CCCP | Sphingobium fuliginis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethyl-parathion + H2O | - |
Sphingobium fuliginis | dimethyl thiophosphate + 4-nitrophenol | - |
? | |
additional information | organophosphate hydrolase interacts with Ton components and is targeted to the membrane only in the presence of the ExbB/ExbD complex | Sphingobium fuliginis | ? | - |
? | |
additional information | OPH is known to cleave the third ester linkage found in structurally diverse groups of organophosphorous compounds | Sphingobium fuliginis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Sphingobium fuliginis |
Synonyms | Comment | Organism |
---|---|---|
opd | - |
Sphingobium fuliginis |
OPH | - |
Sphingobium fuliginis |
organophosphate hydrolase | - |
Sphingobium fuliginis |
parathion hydrolase | UniProt | Sphingobium fuliginis |
General Information | Comment | Organism |
---|---|---|
evolution | Sphingobium fuliginis acquired OPH coding opd gene through lateral gene transfer | Sphingobium fuliginis |
additional information | enzyme three-dimensional structure homology model is used for interaction analysis in silico predictions of biopolymer transport protein ExbD with enzyme OPH, protein-protein docking studies on different variants of OPH and ExbD, overview. Residues R91 and R96 of OPH do contribute to interactions with recombinant N-terminally His6-tagged ExbD. Formation of a four-component Ton complex due to OPH interactions with proton motive force (ExbB/ExbD) and energy harvesting (TonB) components | Sphingobium fuliginis |
physiological function | organophosphate hydrolase interacts with Ton components and is targeted to the membrane only in the presence of the ExbB/ExbD complex. Proton motif force (PMF) is required for efficient uptake and utilization of dimethyl-parathion as sole source of phosphate, no growth is observed in cultures having PMF inhibitor CCCP | Sphingobium fuliginis |