Cloned (Comment) | Organism |
---|---|
gene opdD, DNA and amino acid sequence determination and analysis, genetic structure, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli strain BL21(DE3) | Latilactobacillus sakei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cadusafos + H2O | Latilactobacillus sakei | - |
O,O-diethylphosphorodithioate + phenol | - |
? | |
cadusafos + H2O | Latilactobacillus sakei WCP904 | - |
O,O-diethylphosphorodithioate + phenol | - |
? | |
chlorpyrifos + H2O | Latilactobacillus sakei | - |
O,O-diethylphosphorothioate + 3,5,6-trichloropyridin-2-ol | - |
? | |
chlorpyrifos + H2O | Latilactobacillus sakei WCP904 | - |
O,O-diethylphosphorothioate + 3,5,6-trichloropyridin-2-ol | - |
? | |
coumaphos + H2O | Latilactobacillus sakei | - |
O,O-diethylphosphorothioate + 3-chloro-4-methylumbelliferone | - |
? | |
coumaphos + H2O | Latilactobacillus sakei WCP904 | - |
O,O-diethylphosphorothioate + 3-chloro-4-methylumbelliferone | - |
? | |
diazinon + H2O | Latilactobacillus sakei | - |
O,O-diethylphosphorothioate + ? | - |
? | |
diazinon + H2O | Latilactobacillus sakei WCP904 | - |
O,O-diethylphosphorothioate + ? | - |
? | |
diethyl-parathion + H2O | Latilactobacillus sakei | - |
diethyl thiophosphate + 4-nitrophenol | - |
? | |
dimethyl-parathion + H2O | Latilactobacillus sakei | - |
dimethyl thiophosphate + 4-nitrophenol | - |
? | |
dyfonate + H2O | Latilactobacillus sakei | - |
O,O-diethylphosphorothioate + 2-isopropyl-6-methylpyrimidin-4-ol | - |
? | |
ethoprophos + H2O | Latilactobacillus sakei | - |
? | - |
? | |
fenamiphos + H2O | Latilactobacillus sakei | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Latilactobacillus sakei | - |
isolated from kimchi fermentation, yeolmu-mulkimchi contaminated with chlorpyrifos | - |
Latilactobacillus sakei WCP904 | - |
isolated from kimchi fermentation, yeolmu-mulkimchi contaminated with chlorpyrifos | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain BL21(DE3) | Latilactobacillus sakei |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activity in wild-type strain WCP904 and of purified recombinant enzyme with different organophosphorus pesticides, overview | Latilactobacillus sakei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl butyrate + H2O | - |
Latilactobacillus sakei | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl butyrate + H2O | - |
Latilactobacillus sakei WCP904 | 4-nitrophenol + butyrate | - |
? | |
cadusafos + H2O | - |
Latilactobacillus sakei | O,O-diethylphosphorodithioate + phenol | - |
? | |
cadusafos + H2O | - |
Latilactobacillus sakei WCP904 | O,O-diethylphosphorodithioate + phenol | - |
? | |
chlorpyrifos + H2O | - |
Latilactobacillus sakei | O,O-diethylphosphorothioate + 3,5,6-trichloropyridin-2-ol | - |
? | |
chlorpyrifos + H2O | - |
Latilactobacillus sakei WCP904 | O,O-diethylphosphorothioate + 3,5,6-trichloropyridin-2-ol | - |
? | |
coumaphos + H2O | - |
Latilactobacillus sakei | O,O-diethylphosphorothioate + 3-chloro-4-methylumbelliferone | - |
? | |
coumaphos + H2O | - |
Latilactobacillus sakei WCP904 | O,O-diethylphosphorothioate + 3-chloro-4-methylumbelliferone | - |
? | |
diazinon + H2O | - |
Latilactobacillus sakei | O,O-diethylphosphorothioate + ? | - |
? | |
diazinon + H2O | - |
Latilactobacillus sakei WCP904 | O,O-diethylphosphorothioate + ? | - |
? | |
diethyl-parathion + H2O | - |
Latilactobacillus sakei | diethyl thiophosphate + 4-nitrophenol | - |
? | |
dimethyl-parathion + H2O | - |
Latilactobacillus sakei | dimethyl thiophosphate + 4-nitrophenol | - |
? | |
dyfonate + H2O | - |
Latilactobacillus sakei | O,O-diethylphosphorothioate + 2-isopropyl-6-methylpyrimidin-4-ol | - |
? | |
ethoprophos + H2O | - |
Latilactobacillus sakei | ? | - |
? | |
fenamiphos + H2O | - |
Latilactobacillus sakei | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 31000, recombinant enzyme, SDS-PAGE | Latilactobacillus sakei |
More | the deduced amino acid sequences of OpdD shows a potential serine hydrolase motif such as G-H-S116-Q-G | Latilactobacillus sakei |
Synonyms | Comment | Organism |
---|---|---|
OpdD | - |
Latilactobacillus sakei |
organophosphorus hydrolase | - |
Latilactobacillus sakei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
recombinant enzyme, with substrate 4-nitrophenyl butyrate | Latilactobacillus sakei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
recombinant enzyme, with substrate 4-nitrophenyl butyrate | Latilactobacillus sakei |
General Information | Comment | Organism |
---|---|---|
additional information | the deduced amino acid sequences of OpdD show a potential serine hydrolase motif such as G-H-S116-Q-G | Latilactobacillus sakei |