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Literature summary for 3.1.8.1 extracted from

  • Merone, L.; Mandrich, L.; Porzio, E.; Rossi, M.; Müller, S.; Reiter, G.; Worek, F.; Manco, G.
    Improving the promiscuous nerve agent hydrolase activity of a thermostable archaeal lactonase (2010), Bioresour. Technol., 101, 9204-9212.
    View publication on PubMed
Search for more literature for 3.1.8.1

Activating Compound

Activating Compound Comment Organism Structure
SDS 0.1% (w/v), 25°C, wild type enzyme, 11-fold increase of specific activity, activation is stable up to 40 h. The activating effect is reduced at 70°C Saccharolobus solfataricus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Saccharolobus solfataricus

Protein Variants

Protein Variants Comment Organism
W263F kcat/Km for paraoxon is 6.5fold higher than wild-type value Saccharolobus solfataricus
W263Q kcat/Km for paraoxon is 3.5fold lower than wild-type value Saccharolobus solfataricus
Y97W kcat/Km for paraoxon is 2.4fold higher than wild-type value Saccharolobus solfataricus
Y97W/I261F kcat/Km for paraoxon is 1.4fold higher than wild-type value Saccharolobus solfataricus
Y97W/I98F/I261F kcat/Km for paraoxon is 1.2fold higher than wild-type value Saccharolobus solfataricus
Y97W/W263F kcat/Km for paraoxon is 1.6fold higher than wild-type value Saccharolobus solfataricus
Y97W/W263Q kcat/Km for paraoxon is 1.7fold lower than wild-type value Saccharolobus solfataricus

General Stability

General Stability Organism
stable for up to 40 h in 0.2% SDS at 25°C Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.064
-
 paraoxon pH 8.0, 70°C, wild-type enzyme Saccharolobus solfataricus
0.075
-
 paraoxon pH 8.0, 70°C, mutant enzyme Y97W Saccharolobus solfataricus
0.153
-
 paraoxon pH 8.0, 70°C, mutant enzyme W263F Saccharolobus solfataricus
0.272
-
 paraoxon pH 8.0, 70°C, mutant enzyme Y97W/W263F Saccharolobus solfataricus
0.41
-
 paraoxon pH 8.0, 70°C, mutant enzyme Y97W/I261F Saccharolobus solfataricus
1.2
-
 paraoxon pH 8.0, 70°C, mutant enzyme Y97W/I98F/I261F Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q97VT7
-
-
Saccharolobus solfataricus P2 Q97VT7
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-cyclosarin + H2O wild type enzyme and mutant enzyme W263F hydrolyze the (+)-enantiomer approximately 3 and 4.5 times faster than the (-)-enantiomer Saccharolobus solfataricus methyl-phosphonic acid monofluoride + cyclohexanol
-
 ?
(+)-cyclosarin + H2O wild type enzyme and mutant enzyme W263F hydrolyze the (+)-enantiomer approximately 3 and 4.5 times faster than the (-)-enantiomer Saccharolobus solfataricus P2 methyl-phosphonic acid monofluoride + cyclohexanol
-
 ?
paraoxon + H2O
-
 Saccharolobus solfataricus 4-nitrophenol + diethyl phosphate
-
 ?
paraoxon + H2O
-
 Saccharolobus solfataricus P2 4-nitrophenol + diethyl phosphate
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
phosphotriesterase-like lactonase
-
 Saccharolobus solfataricus
SSO2522 locus name Saccharolobus solfataricus
SsoPox
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
 assay at Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.24
-
 paraoxon pH 8.0, 70°C, wild-type enzyme Saccharolobus solfataricus
0.68
-
 paraoxon pH 8.0, 70°C, mutant enzyme Y97W Saccharolobus solfataricus
1.63
-
 paraoxon pH 8.0, 70°C, mutant enzyme Y97W/W263F Saccharolobus solfataricus
2.2
-
 paraoxon pH 8.0, 70°C, mutant enzyme Y97W/I261F Saccharolobus solfataricus
3.7
-
 paraoxon pH 8.0, 70°C, mutant enzyme Y97W/I98F/I261F Saccharolobus solfataricus
3.76
-
 paraoxon pH 8.0, 70°C, mutant enzyme W263F Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Saccharolobus solfataricus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.07
-
 paraoxon pH 8.0, 70°C, mutant enzyme W263Q Saccharolobus solfataricus
2.2
-
 paraoxon pH 8.0, 70°C, mutant enzyme Y97W/W263Q Saccharolobus solfataricus
2.45
-
 paraoxon pH 8.0, 70°C, mutant enzyme W263F Saccharolobus solfataricus
3.05
-
 paraoxon pH 8.0, 70°C, mutant enzyme Y97W/I98F/I261F Saccharolobus solfataricus
3.75
-
 paraoxon pH 8.0, 70°C, wild-type enzyme Saccharolobus solfataricus
5.4
-
 paraoxon pH 8.0, 70°C, mutant enzyme Y97W/I261F Saccharolobus solfataricus
6.02
-
 paraoxon pH 8.0, 70°C, mutant enzyme Y97W/W263F Saccharolobus solfataricus
9.01
-
 paraoxon pH 8.0, 70°C, mutant enzyme Y97W Saccharolobus solfataricus