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Literature summary for 3.1.8.1 extracted from

  • Porzio, E.; Merone, L.; Mandrich, L.; Rossi, M.; Manco, G.
    A new phosphotriesterase from Sulfolobus acidocaldarius and its comparison with the homologue from Sulfolobus solfataricus (2007), Biochimie, 89, 625-636.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed in Escherichia coli Sulfolobus acidocaldarius

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4
-
methyl paraoxon pH 9.0, 75°C Sulfolobus acidocaldarius

Metals/Ions

Metals/Ions Comment Organism Structure
Cd2+ 0.2 mM, more than 8fold increase in activity Sulfolobus acidocaldarius

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35307
-
x * 35307, calculated from sequence Sulfolobus acidocaldarius
35500
-
x * 35500, SDS-PAGE Sulfolobus acidocaldarius
69100
-
gel filtration Sulfolobus acidocaldarius

Organism

Organism UniProt Comment Textmining
Sulfolobus acidocaldarius Q4J6Z8
-
-
Sulfolobus acidocaldarius DSM 639 Q4J6Z8
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Sulfolobus acidocaldarius

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl butanoate + H2O
-
Sulfolobus acidocaldarius 4-nitrophenol + butanoate
-
?
4-nitrophenyl butanoate + H2O
-
Sulfolobus acidocaldarius DSM 639 4-nitrophenol + butanoate
-
?
diethyl 4-nitrophenyl phosphate + H2O i.e. paraoxon Sulfolobus acidocaldarius 4-nitrophenol + diethyl hydrogen phosphate
-
?
diethyl 4-nitrophenyl phosphate + H2O i.e. paraoxon Sulfolobus acidocaldarius DSM 639 4-nitrophenol + diethyl hydrogen phosphate
-
?
methyl paraoxon + H2O
-
Sulfolobus acidocaldarius 4-nitrophenol + dimethyl phosphate
-
?
methyl paraoxon + H2O
-
Sulfolobus acidocaldarius DSM 639 4-nitrophenol + dimethyl phosphate
-
?
methyl parathion + H2O
-
Sulfolobus acidocaldarius 4-nitrophenol + dimethyl thiophosphate
-
?
methyl parathion + H2O
-
Sulfolobus acidocaldarius DSM 639 4-nitrophenol + dimethyl thiophosphate
-
?

Subunits

Subunits Comment Organism
? x * 35500, SDS-PAGE Sulfolobus acidocaldarius
? x * 35307, calculated from sequence Sulfolobus acidocaldarius

Synonyms

Synonyms Comment Organism
phosphotriesterase
-
Sulfolobus acidocaldarius
SACI2140 locus name Sulfolobus acidocaldarius
SacPox
-
Sulfolobus acidocaldarius

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
-
Sulfolobus acidocaldarius

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 95 50°C: about 50% of maximal activity, 95°C: about 50% of maximal activity Sulfolobus acidocaldarius

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
2 h, no change in activity Sulfolobus acidocaldarius
80
-
2 h, 30% loss of activity Sulfolobus acidocaldarius
85
-
2 h, 35% loss of activity Sulfolobus acidocaldarius
90
-
t1/2: 5 min Sulfolobus acidocaldarius

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.75
-
methyl paraoxon pH 9.0, 75°C Sulfolobus acidocaldarius

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
at 70°C Sulfolobus acidocaldarius

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9.5 at 70°C, pH 6.5: about 30% of maximal activity, pH 9.5: about 35% of maximal activity Sulfolobus acidocaldarius

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.91
-
diethyl 4-nitrophenyl phosphate pH 9.0, 75°C Sulfolobus acidocaldarius
5.57
-
methyl paraoxon pH 9.0, 75°C Sulfolobus acidocaldarius