Literature summary for 3.1.8.1 extracted from

  • Tsai, P.C.; Bigley, A.; Li, Y.; Ghanem, E.; Cadieux, C.L.; Kasten, S.A.; Reeves, T.E.; Cerasoli, D.M.; Raushel, F.M.
    Stereoselective hydrolysis of organophosphate nerve agents by the bacterial phosphotriesterase (2010), Biochemistry, 49, 7978-7987.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL-21(DE3) cells Homo sapiens

Engineering

Protein Variants Comment Organism
S308G the mutant shows decreased catalytic efficiency compared to the wild type enzyme Homo sapiens
G60A the mutant shows increased catalytic efficiency with sarin and soman compared to the wild type enzyme Homo sapiens
I106G the mutant shows decreased catalytic efficiency compared to the wild type enzyme Homo sapiens
F132G the mutant shows decreased catalytic efficiency compared to the wild type enzyme Homo sapiens
H254Q/H257F the mutant shows increased catalytic efficiency compared to the wild type enzyme Homo sapiens
H257Y/L303T the mutant shows decreased catalytic efficiency compared to the wild type enzyme Homo sapiens
I106G/H257Y the mutant shows decreased catalytic efficiency compared to the wild type enzyme Homo sapiens
I106G/F132G/H257Y the mutant shows decreased catalytic efficiency compared to the wild type enzyme Homo sapiens
I106A/F132A/H257Y the mutant shows decreased catalytic efficiency compared to the wild type enzyme Homo sapiens
I106A/H257Y/S308A the mutant shows decreased catalytic efficiency compared to the wild type enzyme Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ the native enzyme contains two Zn2+ ions, and these metal ions can be substituted with Cd2+, Co2+, Ni2+, or Mn2+ without a loss of catalytic activity Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Ultrogel AcA 54 gel filtration, and DEAE-Sephadex A-25 gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the stereochemical preference of the wild-type enzyme with chemical warfare agents is for the RP enantiomers Homo sapiens ?
-
?
soman + H2O
-
Homo sapiens ?
-
?
sarin + H2O
-
Homo sapiens ?
-
?
tabun + H2O
-
Homo sapiens ?
-
?
cyclosarin + H2O
-
Homo sapiens ?
-
?
paraoxon + H2O best substrate Homo sapiens 4-nitrophenol + diethyl phosphate
-
?
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (R)-methylphosphonate + H2O
-
Homo sapiens 4-acetylphenol + (2R)-3,3-dimethylbutan-2-yl (R)-methylphosphonate
-
?
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (S)-methylphosphonate + H2O
-
Homo sapiens 4-acetylphenol + (2R)-3,3-dimethylbutan-2-yl (S)-methylphosphonate
-
?
4-acetylphenyl (2S)-3,3-dimethylbutan-2-yl (R)-methylphosphonate + H2O
-
Homo sapiens 4-acetylphenol + 4-acetylphenol + (2S)-3,3-dimethylbutan-2-yl (R)-methylphosphonate
-
?
4-acetylphenyl (2S)-3,3-dimethylbutan-2-yl (S)-methylphosphonate + H2O
-
Homo sapiens 4-acetylphenol + (2S)-3,3-dimethylbutan-2-yl (S)-methylphosphonate
-
?
4-acetylphenyl 2-methylpropyl (R)-methylphosphonate + H2O
-
Homo sapiens 4-acetylphenol + 2-methylpropyl (R)-methylphosphonate
-
?
4-acetylphenyl 2-methylpropyl (S)-methylphosphonate + H2O
-
Homo sapiens 4-acetylphenol + 2-methylpropyl (S)-methylphosphonate
-
?
4-acetylphenyl cyclohexyl (R)-methylphosphonate + H2O
-
Homo sapiens 4-acetylphenol + cyclohexyl (R)-methylphosphonate
-
?
4-acetylphenyl cyclohexyl (S)-methylphosphonate + H2O
-
Homo sapiens 4-acetylphenol + cyclohexyl (S)-methylphosphonate
-
?
4-acetylphenyl ethyl (R)-methylphosphonate + H2O
-
Homo sapiens 4-acetylphenol + ethyl (R)-methylphosphonate
-
?
4-acetylphenyl ethyl (S)-methylphosphonate + H2O
-
Homo sapiens 4-acetylphenol + ethyl (S)-methylphosphonate
-
?
4-acetylphenyl propan-2-yl (R)-methylphosphonate + H2O
-
Homo sapiens 4-acetylphenol + propan-2-yl (R)-methylphosphonate
-
?
4-acetylphenyl propan-2-yl (S)-methylphosphonate + H2O
-
Homo sapiens 4-acetylphenol + propan-2-yl (S)-methylphosphonate
-
?
VR + H2O
-
Homo sapiens ?
-
?
VX + H2O
-
Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
homodimer
-
Homo sapiens

Synonyms

Synonyms Comment Organism
phosphotriesterase
-
Homo sapiens
PTE
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.41
-
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (R)-methylphosphonate mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
18
-
4-acetylphenyl propan-2-yl (R)-methylphosphonate mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
15
-
sarin mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
15
-
4-acetylphenyl propan-2-yl (S)-methylphosphonate mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.4
-
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (R)-methylphosphonate wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
0.016
-
4-acetylphenyl (2S)-3,3-dimethylbutan-2-yl (S)-methylphosphonate wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
1.5
-
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (S)-methylphosphonate mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
2.6
-
soman wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
120
-
sarin mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
120
-
4-acetylphenyl propan-2-yl (R)-methylphosphonate mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
2
-
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (R)-methylphosphonate mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
2.1
-
4-acetylphenyl (2S)-3,3-dimethylbutan-2-yl (S)-methylphosphonate mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
2.2
-
soman mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
2.2
-
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (R)-methylphosphonate mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
0.21
-
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (S)-methylphosphonate mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
89
-
soman mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
110
-
4-acetylphenyl ethyl (S)-methylphosphonate mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
110
-
4-acetylphenyl 2-methylpropyl (R)-methylphosphonate mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
110
-
4-acetylphenyl ethyl (R)-methylphosphonate mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
2.9
-
4-acetylphenyl (2S)-3,3-dimethylbutan-2-yl (S)-methylphosphonate mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
22
-
4-acetylphenyl 2-methylpropyl (S)-methylphosphonate wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
39
-
sarin mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
0.81
-
4-acetylphenyl cyclohexyl (R)-methylphosphonate mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
7.4
-
4-acetylphenyl propan-2-yl (S)-methylphosphonate mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
20
-
4-acetylphenyl 2-methylpropyl (R)-methylphosphonate mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
25
-
soman mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
7.3
-
4-acetylphenyl ethyl (R)-methylphosphonate mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
50
-
4-acetylphenyl 2-methylpropyl (S)-methylphosphonate mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
0.45
-
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (S)-methylphosphonate wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
130
-
4-acetylphenyl ethyl (R)-methylphosphonate mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
190
-
4-acetylphenyl ethyl (S)-methylphosphonate mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
150
-
4-acetylphenyl cyclohexyl (R)-methylphosphonate mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
150
-
4-acetylphenyl ethyl (R)-methylphosphonate wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
14
-
4-acetylphenyl ethyl (R)-methylphosphonate mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
40
-
4-acetylphenyl propan-2-yl (S)-methylphosphonate wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
19
-
4-acetylphenyl cyclohexyl (S)-methylphosphonate mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
0.12
-
4-acetylphenyl (2S)-3,3-dimethylbutan-2-yl (S)-methylphosphonate mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
100
-
4-acetylphenyl 2-methylpropyl (S)-methylphosphonate mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
100
-
4-acetylphenyl propan-2-yl (R)-methylphosphonate wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
90
-
sarin wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
0.62
-
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (S)-methylphosphonate mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
92
-
4-acetylphenyl propan-2-yl (S)-methylphosphonate mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
8.5
-
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (R)-methylphosphonate mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
12
-
4-acetylphenyl 2-methylpropyl (S)-methylphosphonate mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
250
-
4-acetylphenyl propan-2-yl (R)-methylphosphonate mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
290
-
4-acetylphenyl ethyl (S)-methylphosphonate mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
5.9
-
4-acetylphenyl cyclohexyl (R)-methylphosphonate mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
51
-
4-acetylphenyl 2-methylpropyl (R)-methylphosphonate mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
5.1
-
4-acetylphenyl cyclohexyl (S)-methylphosphonate mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
0.14
-
4-acetylphenyl cyclohexyl (S)-methylphosphonate mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
670
-
4-acetylphenyl ethyl (S)-methylphosphonate wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
9.1
-
soman mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
37
-
4-acetylphenyl 2-methylpropyl (R)-methylphosphonate mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
93
-
4-acetylphenyl cyclohexyl (R)-methylphosphonate mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
93
-
4-acetylphenyl 2-methylpropyl (R)-methylphosphonate wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
300
-
sarin mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
410
-
4-acetylphenyl ethyl (S)-methylphosphonate mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
370
-
4-acetylphenyl propan-2-yl (S)-methylphosphonate mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens

KCat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
16
-
4-acetylphenyl cyclohexyl (R)-methylphosphonate wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
2.5
-
4-acetylphenyl propan-2-yl (R)-methylphosphonate mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
130
-
4-acetylphenyl ethyl (S)-methylphosphonate mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
6.2
-
4-acetylphenyl propan-2-yl (S)-methylphosphonate mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
5.9
-
4-acetylphenyl propan-2-yl (S)-methylphosphonate mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
0.2
-
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (S)-methylphosphonate wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
110
-
4-acetylphenyl propan-2-yl (S)-methylphosphonate mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
110
-
4-acetylphenyl propan-2-yl (R)-methylphosphonate mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
2.4
-
4-acetylphenyl ethyl (R)-methylphosphonate mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
1200
-
4-acetylphenyl ethyl (S)-methylphosphonate wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
5.8
-
4-acetylphenyl cyclohexyl (S)-methylphosphonate mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
28
-
4-acetylphenyl cyclohexyl (S)-methylphosphonate mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
28
-
4-acetylphenyl cyclohexyl (R)-methylphosphonate mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
1.5
-
4-acetylphenyl 2-methylpropyl (R)-methylphosphonate mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
1.5
-
4-acetylphenyl ethyl (R)-methylphosphonate mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
1.8
-
4-acetylphenyl propan-2-yl (R)-methylphosphonate mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
2.3
-
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (R)-methylphosphonate mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
0.49
-
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (S)-methylphosphonate mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
0.021
-
4-acetylphenyl cyclohexyl (S)-methylphosphonate wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
0.22
-
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (R)-methylphosphonate mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
520
-
4-acetylphenyl ethyl (R)-methylphosphonate mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
520
-
4-acetylphenyl propan-2-yl (R)-methylphosphonate mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
1.3
-
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (R)-methylphosphonate wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
0.13
-
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (S)-methylphosphonate mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
21
-
4-acetylphenyl cyclohexyl (R)-methylphosphonate mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
0.003
-
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (R)-methylphosphonate mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
3.1
-
4-acetylphenyl propan-2-yl (S)-methylphosphonate mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
0.0032
-
4-acetylphenyl (2S)-3,3-dimethylbutan-2-yl (S)-methylphosphonate wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
150
-
4-acetylphenyl ethyl (S)-methylphosphonate mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
34
-
4-acetylphenyl 2-methylpropyl (S)-methylphosphonate wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
0.016
-
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (S)-methylphosphonate mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
0.0011
-
4-acetylphenyl (2S)-3,3-dimethylbutan-2-yl (S)-methylphosphonate mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
160
-
4-acetylphenyl ethyl (S)-methylphosphonate mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
0.0067
-
4-acetylphenyl (2S)-3,3-dimethylbutan-2-yl (S)-methylphosphonate mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
0.25
-
4-acetylphenyl cyclohexyl (R)-methylphosphonate mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
0.25
-
4-acetylphenyl (2S)-3,3-dimethylbutan-2-yl (S)-methylphosphonate mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
13
-
4-acetylphenyl 2-methylpropyl (R)-methylphosphonate mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
0.1
-
4-acetylphenyl cyclohexyl (S)-methylphosphonate mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
180
-
4-acetylphenyl 2-methylpropyl (S)-methylphosphonate mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
180
-
4-acetylphenyl 2-methylpropyl (R)-methylphosphonate mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
1.7
-
4-acetylphenyl (2S)-3,3-dimethylbutan-2-yl (S)-methylphosphonate mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
0.59
-
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (S)-methylphosphonate mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
1.9
-
4-acetylphenyl cyclohexyl (R)-methylphosphonate mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
220
-
4-acetylphenyl ethyl (S)-methylphosphonate mutant enzyme H254G/H257W/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
75
-
4-acetylphenyl ethyl (R)-methylphosphonate mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
27
-
4-acetylphenyl propan-2-yl (S)-methylphosphonate wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
490
-
4-acetylphenyl ethyl (R)-methylphosphonate wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
9.5
-
4-acetylphenyl 2-methylpropyl (S)-methylphosphonate mutant enzyme S308G, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
73
-
4-acetylphenyl 2-methylpropyl (S)-methylphosphonate mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
760
-
4-acetylphenyl 2-methylpropyl (R)-methylphosphonate mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
0.00094
-
4-acetylphenyl cyclohexyl (S)-methylphosphonate mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
580
-
4-acetylphenyl propan-2-yl (R)-methylphosphonate wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
850
-
4-acetylphenyl 2-methylpropyl (R)-methylphosphonate wild type enzyme, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
0.058
-
4-acetylphenyl (2R)-3,3-dimethylbutan-2-yl (R)-methylphosphonate mutant enzyme H275Y/L303T, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens
0.099
-
4-acetylphenyl 2-methylpropyl (S)-methylphosphonate mutant enzyme G60A, in 50 mM CHES (pH 9.0), at 30°C Homo sapiens