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Literature summary for 3.1.8.1 extracted from

  • Gencer, N.; Arslan, O.
    Purification human PON1Q192 and PON1R192 isoenzymes by hydrophobic interaction chromatography and investigation of the inhibition by metals (2009), J. Chromatogr. B, 877, 134-140.
    View publication on PubMed
Search for more literature for 3.1.8.1

Inhibitors

Inhibitors Comment Organism Structure
Cd2+ noncompetitive inhibition of isoenzyme Q192 and uncompetitive inhibition of isoenzyme R192 Homo sapiens
Co2+ competitive inhibition of isoenzyme Q192 and R192 Homo sapiens
Cu2+ competitive inhibition of isoenzyme Q192 and R192 Homo sapiens
Hg2+ noncompetitive inhibition of isoenzymes Q192 and R192 Homo sapiens
Mn2+ competitive inhibition of isoenzyme Q192 and R192 Homo sapiens
additional information metals are more effective inhibitors on purified PON1-R192 activity than PON1-Q192 activity Homo sapiens
Ni2+ competitive inhibition of isoenzyme Q192 and R192 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.49 2 paraoxon R192 isoenzyme, at pH 8.0 and 37°C Homo sapiens
0.599
-
 paraoxon Q192 isoenzyme, at pH 8.0 and 37°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required for the catalysis Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 x * 43000, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by ammonium sulfate precipitation and hydrophobic interaction chromatography specifically designed for PON1 enzyme, 901fold with 14.5% yield for R192 isoenzyme and 453fold with 6.9% yield for Q192 isoenzyme Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
serum
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.76
-
 Q192 isoenzyme, serum, pH 8 at 37°C, in Tris-base buffer Homo sapiens
5.21
-
 R192 isoenzyme, serum, pH 8 at 37°C, in Tris-base buffer Homo sapiens
2109
-
 Q192 isoenzyme, 901fold purified, pH 8 at 37°C, in Tris-base buffer Homo sapiens
3333
-
 R192 isoenzyme, 453fold purified, pH 8 at 37°C, in Tris-base buffer Homo sapiens

Storage Stability

Storage Stability Organism
4°C, 2 mM CaCl2 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
paraoxon + H2O
-
 Homo sapiens 4-nitrophenol + diethyl phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 43000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
paraoxonase
-
 Homo sapiens
PON1
-
 Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.061
-
 R192 isoenzyme, pH 8 at 25°C Homo sapiens Cu2+
0.106
-
 R192 isoenzyme, pH 8 at 25°C Homo sapiens Hg2+
0.152
-
 R192 isoenzyme, pH 8 at 25°C Homo sapiens Cd2+
0.218
-
 Q192 isoenzyme, pH 8 at 25°C Homo sapiens Cd2+
0.304
-
 R192 isoenzyme, pH 8 at 25°C Homo sapiens Mn2+
0.31
-
 Q192 isoenzyme, pH 8 at 25°C Homo sapiens Cu2+
0.609
-
 Q192 isoenzyme, pH 8 at 25°C Homo sapiens Mn2+
0.781
-
 R192 isoenzyme, pH 8 at 25°C Homo sapiens Co2+
0.891
-
 Q192 isoenzyme, pH 8 at 25°C Homo sapiens Hg2+
1.026
-
 R192 isoenzyme, pH 8 at 25°C Homo sapiens Ni2+
1.144
-
 Q192 isoenzyme, pH 8 at 25°C Homo sapiens Ni2+
3.91
-
 Q192 isoenzyme, pH 8 at 25°C Homo sapiens Co2+