Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetaminophen | at 37°C, 5% slightly reduces activity by 18% concentration | Homo sapiens | |
acetone | at 37°C, 1 mM reduces activity by 82% | Homo sapiens | |
acetyl salicylic acid | at 37°C, 5% slightly reduces activity by 10% concentration | Homo sapiens | |
beta-mercaptoethanol | at 37°C, 1 mM reduces activity by 52% | Homo sapiens | |
Cd2+ | at 37°C, 1 mM inhibits activity | Homo sapiens | |
Co2+ | at 37°C, 1 mM reduces activity by 30% | Homo sapiens | |
Cu2+ | at 37°C, 1 mM inhibits activity | Homo sapiens | |
dimethyl sulfoxide | at 37°C, 5% completely inhibits activity | Homo sapiens | |
dimethylformamide | at 37°C, 5% completely inhibits activity | Homo sapiens | |
ethanol | at 37°C, 1 mM reduces activity by 43% | Homo sapiens | |
gentamicin sulfate | at 37°C, 0.6 mg/ml reduces activity by 50% | Homo sapiens | |
iodoacetic acid | at 37°C, 5% completely inhibits activity | Homo sapiens | |
kanamycin sulfate | at 37°C, 0.6 mg/ml reduces activity by 50% | Homo sapiens | |
additional information | at 37°C, 1 mM Mg2+, 1 mM Mn2+, 5% glycerol, 5% PEG 6000, 5% ammonium sulfate, and 5% phenylmethylsulfonyl fluoride do not inhibit the enzyme activity | Homo sapiens | |
Ni2+ | at 37°C, 1 mM inhibits activity | Homo sapiens | |
Pb2+ | at 37°C, 1 mM reduces activity by 35% | Homo sapiens | |
sodium ampicillin | at 37°C, 30 mg/ml reduces activity by 50% | Homo sapiens | |
Sodium dodecyl sulfate | at 37°C, 5% completely inhibits activity | Homo sapiens | |
streptomycin sulfate | at 37°C, 0.6 mg/ml reduces activity by 50% | Homo sapiens | |
Zn2+ | at 37°C, 1 mM reduces activity by 70% | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
paraoxon | 2 mM substrate, at pH 8.0 and 37°C | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | at 37°C, 1 mM Mg2+ and Mn2+ have no stimulatory effect on activity | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
x * 45000, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by Triton-X-100-treatment, ammonium sulfate precipitation, cholesterol-conjugated magnetic nanoparticles and gel filtration, at 4°C, to homogeneity, 515fold with 73% yield | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
plasma | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.85 | - |
plasma, at 37°C | Homo sapiens |
438.2 | - |
515fold purified enzyme, at 37°C | Homo sapiens |
Storage Stability | Organism |
---|---|
4°C, 20% glycerol | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
paraoxon + H2O | - |
Homo sapiens | 4-nitrophenol + diethyl phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 45000, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
paraoxonase 1 | - |
Homo sapiens |
PON1 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
is stable for 8 to 10 h at 37°C. Completely loses activity at 50°C | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Homo sapiens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
activity is reduced below pH 7.0 and at alkaline pH | Homo sapiens |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Homo sapiens | purified PON1, isoelectric focusing | - |
5.3 |