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Literature summary for 3.1.8.1 extracted from

  • Harel, M.; Aharoni, A.; Gaidukov, L.; Brumshtein, B.; Khersonsky, O.; Meged, R.; Dvir, H.; Ravelli, R.B.G.; McCarthy, A.; Toker, L.; Silman, I.; Sussman, J.L.; Tawfik, D.S.
    Structure and evolution of the serum paraoxonase family of detoxifying and anti-atherosclerotic enzymes (2004), Nat. Struct. Mol. Biol., 11, 412-419.
    View publication on PubMed
Search for more literature for 3.1.8.1

Cloned(Commentary)

Cloned (Comment) Organism
expression of PON1 genetic variant G2E6 as thioredoxin-fusion protein, fusion via a His6-linker, in Escherichia coli Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant wild-type and selenomethionine-labeled PON1 genetic variant G2E6 by use of different mother liquors, microbatch method, X-ray diffracion structure determination and analysis at 2.2-2.6 A resolution Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular the serum enzyme is secreted to blood from liver, association to HDL Homo sapiens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ involved in catalysis, active site structure, overview Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens the serum paraoxonase family contains detoxifying and anti-atherosclerotic enzymes ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P27169 isozymes PON1-3
-

Purification (Commentary)

Purification (Comment) Organism
recombinant thioredoxin-fusion PON1 genetic variant G2E6 from Escherichia coli, the tag is spontaneously cleaved Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
an aryl dialkyl phosphate + H2O = dialkyl phosphate + an aryl alcohol catalytic mechanism involving His134 and His115 as well as a Ca2+ ion, active site structure, overview Homo sapiens
a

Source Tissue

Source Tissue Comment Organism Textmining
liver the serum enzyme is secreted to blood from liver Homo sapiens
-
serum the serum enzyme is secreted to blood from liver Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the serum paraoxonase family contains detoxifying and anti-atherosclerotic enzymes Homo sapiens ?
-
 ?
additional information the enzyme associates to HDL in the blood stream Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
More tertiary structure/three-dimensional analysis of isozymes PON1-3, anchoring to HDL, overview Homo sapiens

Synonyms

Synonyms Comment Organism
More the enzyme belongs to the the serum paraoxonase PON family, family member overview Homo sapiens
PON
-
 Homo sapiens
serum paraoxonase
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9.4
-
 Homo sapiens