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Literature summary for 3.1.7.6 extracted from
- A soluble form of phosphatase in Saccharomyces cerevisiae capable of converting farnesyl diphosphate into E,E-farnesol (2006), Appl. Biochem. Biotechnol., 128, 149-158.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Triton X-100 | 0.05 g/l, stimulates | Saccharomyces cerevisiae |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 2 mM, 92% inhibition. 0.06 mM, no inhibition | Saccharomyces cerevisiae | |
| Mn2+ | 2 mM, 93% inhibition | Saccharomyces cerevisiae | |
| additional information | 2 mM CuSO4 shows no effect | Saccharomyces cerevisiae | |
| phosphate | 2 mM, 85% inhibition | Saccharomyces cerevisiae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 2 mM, 2.2fold activation | Saccharomyces cerevisiae | |
| additional information | 2 mM CuSO4 shows no effect | Saccharomyces cerevisiae | |
| Zn2+ | 2 mM, 1.4fold activation | Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
x * 60000, SDS-PAGE | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2E,6E)-farnesyl diphosphate + H2O | Saccharomyces cerevisiae | biosynthesis of acyclic sesquiterpenoids | (2E,6E)-farnesol + diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2E,6E)-farnesyl diphosphate + H2O | biosynthesis of acyclic sesquiterpenoids | Saccharomyces cerevisiae | (2E,6E)-farnesol + diphosphate | - |
? | |
| (2E,6E)-farnesyl diphosphate + H2O | the enzyme also hydrolyzes geranyl diphosphate and geranylgeranyl diphosphate to the corresponding alcohols | Saccharomyces cerevisiae | (2E,6E)-farnesol + diphosphate | - |
? | |
| p-nitrophenyl phosphate + H2O | - |
Saccharomyces cerevisiae | p-nitrophenol + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 60000, SDS-PAGE | Saccharomyces cerevisiae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Pho8DELTA62 | the purified phosphatase is a truncated Pho8 lacking 62 amino acids at the N-terminus and is designated Pho8DELTA62N | Saccharomyces cerevisiae |
| pNPP/FPP phosphatase | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Saccharomyces cerevisiae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
maximal activity at pH 7.0, less than 1% of maximal activity at pH 6.0, less than 10% of maximal activity at pH 8.5 | Saccharomyces cerevisiae |
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