Cloned (Comment) | Organism |
---|---|
expression of fragment proteins in Escherichia coli BL21(DE3) | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
D81A | loss of hydrolytic activity with retention of synthesis | Mycobacterium tuberculosis |
DElTA1-86/DELTA395-738 | contains only (p)ppGpp synthesis activity, no hydrolysis activity | Mycobacterium tuberculosis |
DELTA182-738 | fragment contains only (p)ppGpp hydrolysis activity, no synthesis activity | Mycobacterium tuberculosis |
DELTA395-738 | frament contains both synthesis and hydrolysis activities | Mycobacterium tuberculosis |
G241E | loss of synthetic activity and retention of hydrolysis | Mycobacterium tuberculosis |
H344Y | loss of synthetic activity and retention of hydrolysis | Mycobacterium tuberculosis |
H80A | loss of hydrolytic activity with retention of synthesis | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
fragments of the relMtb protein | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pppGpp + H2O | - |
Mycobacterium tuberculosis | pppG + diphosphate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
(p)ppGpp synthetase/hydrolase | - |
Mycobacterium tuberculosis |