Application | Comment | Organism |
---|---|---|
medicine | RelMtb is a key factor in Mycobacterium tuberculosis pathogenesis by regulating th intracellular concentration of (p)ppGpp. | Mycobacterium tuberculosis |
Cloned (Comment) | Organism |
---|---|
the pET22b expression system is used | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.41 | - |
ppGpp | pH 8.0, 30°C, KM is increased 3fold in the presence of the complex containing tRNA, ribosomes, and mRNA | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | required, the optimal concentration is a 2-3fold molar excess over the nucleotide substrate, the addition in excess of optimal concentration decreases the rate of hydrolysis | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ppGpp + H2O | Mycobacterium tuberculosis | i.e. guanosine-3',5'-bis(diphosphate) | Gpp + diphosphate | i.e. guanosine 5'-diphosphate | r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
guanosine 3',5'-bis(diphosphate) + H2O = GDP + diphosphate | RelMtb is a dual-function enzyme carrying out ATP: GTP/GDP/ITP 3-diphosphoryltransferase and (p)ppGpp 3-diphosphohydrolase reactions. The differential regulation of the opposing activities of RelMtb is dependent on the ratio of uncharged to charged tRNA and the association of enzyme with a complex containing tRNA, ribosomes, and mRNA, i.e. RAC | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ppGpp + H2O | i.e. guanosine-3',5'-bis(diphosphate) | Mycobacterium tuberculosis | Gpp + diphosphate | i.e. guanosine 5'-diphosphate | ? | |
ppGpp + H2O | i.e. guanosine-3',5'-bis(diphosphate) | Mycobacterium tuberculosis | Gpp + diphosphate | i.e. guanosine 5'-diphosphate | r |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.97 | - |
ppGpp | pH 8.0, 30°C, kcat is reduced 2fold in the presence of the complex containing tRNA, ribosomes, and mRNA | Mycobacterium tuberculosis | |
3 | 6 | ppGpp | pH 8.0, 30°C, kcat is reduced 2fold in the presence of the complex containing tRNA, ribosomes, and mRNA | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.2 | - |
Mycobacterium tuberculosis |