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Literature summary for 3.1.7.2 extracted from

  • Avarbock, D.; Avarbock, A.; Rubin, H.
    Differential regulation of opposing RelMtb activities by the aminoacylation state of a tRNA ribosome mRNA elMtb complex (2000), Biochemistry, 39, 11640-11648.
    View publication on PubMed

Application

Application Comment Organism
medicine RelMtb is a key factor in Mycobacterium tuberculosis pathogenesis by regulating th intracellular concentration of (p)ppGpp. Mycobacterium tuberculosis

Cloned(Commentary)

Cloned (Comment) Organism
the pET22b expression system is used Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.41
-
ppGpp pH 8.0, 30°C, KM is increased 3fold in the presence of the complex containing tRNA, ribosomes, and mRNA Mycobacterium tuberculosis

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ required, the optimal concentration is a 2-3fold molar excess over the nucleotide substrate, the addition in excess of optimal concentration decreases the rate of hydrolysis Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ppGpp + H2O Mycobacterium tuberculosis i.e. guanosine-3',5'-bis(diphosphate) Gpp + diphosphate i.e. guanosine 5'-diphosphate r

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-

Reaction

Reaction Comment Organism Reaction ID
guanosine 3',5'-bis(diphosphate) + H2O = GDP + diphosphate RelMtb is a dual-function enzyme carrying out ATP: GTP/GDP/ITP 3’-diphosphoryltransferase and (p)ppGpp 3’-diphosphohydrolase reactions. The differential regulation of the opposing activities of RelMtb is dependent on the ratio of uncharged to charged tRNA and the association of enzyme with a complex containing tRNA, ribosomes, and mRNA, i.e. RAC Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ppGpp + H2O i.e. guanosine-3',5'-bis(diphosphate) Mycobacterium tuberculosis Gpp + diphosphate i.e. guanosine 5'-diphosphate ?
ppGpp + H2O i.e. guanosine-3',5'-bis(diphosphate) Mycobacterium tuberculosis Gpp + diphosphate i.e. guanosine 5'-diphosphate r

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.97
-
ppGpp pH 8.0, 30°C, kcat is reduced 2fold in the presence of the complex containing tRNA, ribosomes, and mRNA Mycobacterium tuberculosis
3 6 ppGpp pH 8.0, 30°C, kcat is reduced 2fold in the presence of the complex containing tRNA, ribosomes, and mRNA Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.2
-
Mycobacterium tuberculosis