Literature summary for 3.1.7.11 extracted from

Chen, F.; Li, W.; Jiang, L.; Pu, X.; Yang, Y.; Zhang, G.; Luo, Y.
Functional characterization of a geraniol synthase-encoding gene from Camptotheca acuminata and its application in production of geraniol in Escherichia coli (2016), J. Ind. Microbiol. Biotechnol., 43, 1281-1292 .

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Application

Application	Comment	Organism
synthesis	significant increase in geraniol synthesis is achieved by coexpression of geraniol synthase, mutant S80F of farnesyl diphosphate synthase, and the encoding genes involved in the whole mevalonic acid biosynthetic pathway in Escherichia coli. The additional optimization of medium composition, fermentation time, and addition of metal ions leads to the geraniol production of 48.5 mg/l	Camptotheca acuminata

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Camptotheca acuminata

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0895	-	geranyl diphosphate	pH 7.5, 32°C	Camptotheca acuminata

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	both Mn2+ and Mg2+ are required, optimum concentrations are 1 mM Mg2+ and 0.1 mM Mn2+	Camptotheca acuminata
Mn2+	both Mn2+ and Mg2+ are required, optimum concentrations are 1 mM Mg2+ and 0.1 mM Mn2+	Camptotheca acuminata

Organism

Organism	UniProt	Comment	Textmining
Camptotheca acuminata	A0A109P1X1	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
geranyl diphosphate + H2O	-	Camptotheca acuminata	geraniol + diphosphate	-	?

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
5	25	stable for 30 min	Camptotheca acuminata
45	-	30 min, 50% loss of activity	Camptotheca acuminata

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	-	Camptotheca acuminata

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Release 2023.1 (January 2023)