BRENDA - Enzyme Database show
show all sequences of 3.1.6.9

Specificity studies of bacterial sulfatases by means of structurally defined sulfated oligosaccharides isolated from shark cartilage chondroitin sulfate D

Sugahara, K.; Kojima, T.; Eur. J. Biochem. 239, 865-870 (1996)

Data extracted from this reference:

Application
Application
Commentary
Organism
analysis
the enzyme is a useful tool for structural studies of chondroitin sulfate and dermetan sulfate
Proteus vulgaris
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Proteus vulgaris
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-deoxy-alpha-L-threo-hex-4-enepyranosyluronic acid-beta-(1-3)-GalNAc4SO3--beta-(1-4)-glucuronic acid-2-sulfate-beta-(1-3)-GalNAc6SO3-beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc4SO3- + H2O
removal of two sulfate groups
135539
Proteus vulgaris
sulfate + 4-deoxy-alpha-L-threo-hex-4-enepyranosyluronic acid-beta-(1-3)-GalNAc-beta-(1-4)-glucuronic acid-2-sulfate-beta-(1-3)-GalNAc6SO3-beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc
-
135539
Proteus vulgaris
?
4-deoxy-alpha-L-threo-hex-4-enepyranosyluronic acid-beta-(1-3)-GalNAc6SO3--beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc4SO3-beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc4SO3- + H2O
removal of two sulfate groups
135539
Proteus vulgaris
sulfate + 4-deoxy-alpha-L-threo-hex-4-enepyranosyluronic acid-beta-(1-3)-GalNAc6SO3--beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc-beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc
-
135539
Proteus vulgaris
?
4-deoxy-alpha-L-threo-hex-4-enepyranosyluronic acid-beta-(1-3)-GalNAc6SO3-beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc6SO3-beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc4SO3- + H2O
removal of one sulfate group
135539
Proteus vulgaris
sulfate + 4-deoxy-alpha-L-threo-hex-4-enepyranosyluronic acid-beta-(1-3)-GalNAc6SO3-beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc6SO3-beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc
-
135539
Proteus vulgaris
?
4-deoxy-alpha-L-threo-hex-4-enepyranosyluronic acid2SO3--beta-(1-3)-GalNAc6SO3--beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc4SO3-beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc4SO3- + H2O
removal of two sulfate groups
135539
Proteus vulgaris
sulfate + 4-deoxy-alpha-L-threo-hex-4-enepyranosyluronic acid2SO3--beta-(1-3)-GalNAc6SO3--beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc-beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc
-
-
-
?
4-deoxy-alpha-L-threo-hex-4-enepyranosyluronic acid2SO3--beta-(1-3)-GalNAc6SO3--beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc6SO3-beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc4SO3- + H2O
removal of one sulfate group
135539
Proteus vulgaris
sulfate + 4-deoxy-alpha-L-threo-hex-4-enepyranosyluronic acid2SO3--beta-(1-3)-GalNAc6SO3--beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc6SO3-beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc
-
135539
Proteus vulgaris
?
additional information
the enzyme acts preferentially on an ester sulfate on the C4 position of the GalNAc residue at the reducing end under mild incubation conditions, but also releases a sulfate group under harsh conditions from the C4 position of the GalNAc residue at the internal positions of the hexasaccharide chains, unless the GalNAc residue has another ester sulfate on its C6 position
135539
Proteus vulgaris
?
-
-
-
-
Application (protein specific)
Application
Commentary
Organism
analysis
the enzyme is a useful tool for structural studies of chondroitin sulfate and dermetan sulfate
Proteus vulgaris
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-deoxy-alpha-L-threo-hex-4-enepyranosyluronic acid-beta-(1-3)-GalNAc4SO3--beta-(1-4)-glucuronic acid-2-sulfate-beta-(1-3)-GalNAc6SO3-beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc4SO3- + H2O
removal of two sulfate groups
135539
Proteus vulgaris
sulfate + 4-deoxy-alpha-L-threo-hex-4-enepyranosyluronic acid-beta-(1-3)-GalNAc-beta-(1-4)-glucuronic acid-2-sulfate-beta-(1-3)-GalNAc6SO3-beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc
-
135539
Proteus vulgaris
?
4-deoxy-alpha-L-threo-hex-4-enepyranosyluronic acid-beta-(1-3)-GalNAc6SO3--beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc4SO3-beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc4SO3- + H2O
removal of two sulfate groups
135539
Proteus vulgaris
sulfate + 4-deoxy-alpha-L-threo-hex-4-enepyranosyluronic acid-beta-(1-3)-GalNAc6SO3--beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc-beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc
-
135539
Proteus vulgaris
?
4-deoxy-alpha-L-threo-hex-4-enepyranosyluronic acid-beta-(1-3)-GalNAc6SO3-beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc6SO3-beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc4SO3- + H2O
removal of one sulfate group
135539
Proteus vulgaris
sulfate + 4-deoxy-alpha-L-threo-hex-4-enepyranosyluronic acid-beta-(1-3)-GalNAc6SO3-beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc6SO3-beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc
-
135539
Proteus vulgaris
?
4-deoxy-alpha-L-threo-hex-4-enepyranosyluronic acid2SO3--beta-(1-3)-GalNAc6SO3--beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc4SO3-beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc4SO3- + H2O
removal of two sulfate groups
135539
Proteus vulgaris
sulfate + 4-deoxy-alpha-L-threo-hex-4-enepyranosyluronic acid2SO3--beta-(1-3)-GalNAc6SO3--beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc-beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc
-
-
-
?
4-deoxy-alpha-L-threo-hex-4-enepyranosyluronic acid2SO3--beta-(1-3)-GalNAc6SO3--beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc6SO3-beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc4SO3- + H2O
removal of one sulfate group
135539
Proteus vulgaris
sulfate + 4-deoxy-alpha-L-threo-hex-4-enepyranosyluronic acid2SO3--beta-(1-3)-GalNAc6SO3--beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc6SO3-beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc
-
135539
Proteus vulgaris
?
additional information
the enzyme acts preferentially on an ester sulfate on the C4 position of the GalNAc residue at the reducing end under mild incubation conditions, but also releases a sulfate group under harsh conditions from the C4 position of the GalNAc residue at the internal positions of the hexasaccharide chains, unless the GalNAc residue has another ester sulfate on its C6 position
135539
Proteus vulgaris
?
-
-
-
-
Other publictions for EC 3.1.6.9
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
703926
Akatsu
Development of a mouse monoclo ...
Proteus vulgaris
Glycoconj. J.
27
387-399
2010
-
-
-
-
-
-
-
-
-
-
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3
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1
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1
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-
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-
135539
Sugahara
Specificity studies of bacteri ...
Proteus vulgaris
Eur. J. Biochem.
239
865-870
1996
-
1
-
-
-
-
-
-
-
-
-
-
-
4
-
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6
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1
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6
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170834
Guthrie
Evidence that the Bacteroides ...
Bacteroides thetaiotaomicron
J. Bacteriol.
169
1192-1199
1987
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1
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1
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3
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1
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1
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1
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1
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