BRENDA - Enzyme Database
show all sequences of 3.1.6.8

Saposin B-dependent reconstitution of arylsulfatase a activity in vitro and in cell culture models of metachromatic leukodystrophy

Matzner, U.; Breiden, B.; Schwarzmann, G.; Yaghootfam, A.; Fluharty, A.L.; Hasilik, A.; Sandhoff, K.; Gieselmann, V.; J. Biol. Chem. 284, 9372-9381 (2009)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
bis(monoacylglycero) phosphate
at a relative concentration of 30 mol% the lipid stimulates the reaction rate by a factor of 3.4
Homo sapiens
dolichol
at a relative concentration of 30 mol% the lipid stimulates the reaction rate by a factor of 2.7
Homo sapiens
additional information
phosphatidylinositol and phosphatidylserine have no effect on the rate of sulfatide hydrolysis
Homo sapiens
phosphatidic acid
at a relative concentration of 30 mol% the lipid stimulates the reaction rate by a factor of 3.1
Homo sapiens
Cloned(Commentary)
Commentary
Organism
expressed in baby hamster kidney cells and CHO cells
Homo sapiens
Inhibitors
Inhibitors
Commentary
Organism
Structure
Ca2+
CaCl2 reduces the reaction rate in the low millimolar range
Homo sapiens
Mg2+
MgCl2 reduces the reaction rate in the low millimolar range
Homo sapiens
Mn2+
MnCl2 reduces the reaction rate in the low millimolar range
Homo sapiens
Na+
enzymatic activity is inhibited at NaCl concentrations exceeding 20 mM, at 50 and 200 mM the rate of sulfatide hydrolysis is diminished by about 50 and 75%, respectively
Homo sapiens
phosphate
competitive inhibitor
Homo sapiens
sulfite
competitive inhibitor
Homo sapiens
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
lysosome
-
Homo sapiens
5764
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-nitrocatechol sulfate + H2O
-
693260
Homo sapiens
4-nitrocatechol + sulfate
-
-
-
?
4-nitrophenyl sulfate + H2O
-
693260
Homo sapiens
4-nitrophenol + sulfate
-
-
-
?
cerebroside 3-sulfate + H2O
the reaction requires saposin B, a non-enzymatic proteinaceous cofactor which presents sulfatide to the catalytic site of arylsulfatase A, desulfation is negligible when Sap B is substituted by Sap A, C, or D
693260
Homo sapiens
cerebroside + sulfate
-
-
-
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.3
-
narrow pH optimum around pH 4.3
Homo sapiens
pH Range
pH Minimum
pH Maximum
Commentary
Organism
3.75
4.75
desulfation activity is less than half-maximum at pH values below pH 3.75 and above pH 4.75
Homo sapiens
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
bis(monoacylglycero) phosphate
at a relative concentration of 30 mol% the lipid stimulates the reaction rate by a factor of 3.4
Homo sapiens
dolichol
at a relative concentration of 30 mol% the lipid stimulates the reaction rate by a factor of 2.7
Homo sapiens
additional information
phosphatidylinositol and phosphatidylserine have no effect on the rate of sulfatide hydrolysis
Homo sapiens
phosphatidic acid
at a relative concentration of 30 mol% the lipid stimulates the reaction rate by a factor of 3.1
Homo sapiens
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in baby hamster kidney cells and CHO cells
Homo sapiens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ca2+
CaCl2 reduces the reaction rate in the low millimolar range
Homo sapiens
Mg2+
MgCl2 reduces the reaction rate in the low millimolar range
Homo sapiens
Mn2+
MnCl2 reduces the reaction rate in the low millimolar range
Homo sapiens
Na+
enzymatic activity is inhibited at NaCl concentrations exceeding 20 mM, at 50 and 200 mM the rate of sulfatide hydrolysis is diminished by about 50 and 75%, respectively
Homo sapiens
phosphate
competitive inhibitor
Homo sapiens
sulfite
competitive inhibitor
Homo sapiens
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
lysosome
-
Homo sapiens
5764
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-nitrocatechol sulfate + H2O
-
693260
Homo sapiens
4-nitrocatechol + sulfate
-
-
-
?
4-nitrophenyl sulfate + H2O
-
693260
Homo sapiens
4-nitrophenol + sulfate
-
-
-
?
cerebroside 3-sulfate + H2O
the reaction requires saposin B, a non-enzymatic proteinaceous cofactor which presents sulfatide to the catalytic site of arylsulfatase A, desulfation is negligible when Sap B is substituted by Sap A, C, or D
693260
Homo sapiens
cerebroside + sulfate
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.3
-
narrow pH optimum around pH 4.3
Homo sapiens
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
3.75
4.75
desulfation activity is less than half-maximum at pH values below pH 3.75 and above pH 4.75
Homo sapiens
Other publictions for EC 3.1.6.8
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
751364
Issa
Clinical, molecular, and comp ...
Homo sapiens
J. Mol. Neurosci.
66
17-25
2018
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1
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1
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1
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-
750722
Boehringer
Enzymatic characterization of ...
Homo sapiens
Hum. Mutat.
38
1511-1520
2017
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1
1
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1
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1
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708615
Schroeder
Site-specific analysis of N-li ...
Homo sapiens
Glycobiology
20
248-259
2010
-
1
1
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1
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1
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1
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1
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1
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1
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1
-
-
-
-
-
-
-
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-
2
2
-
-
-
714779
Fujita
Mammalian arylsulfatase A func ...
Mus musculus
Connect. Tissue Res.
51
388-396
2010
-
1
1
-
1
-
-
-
1
-
-
1
-
1
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1
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1
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1
1
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1
1
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1
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1
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1
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1
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
3
3
-
-
-
714958
Poeppel
Misfolded endoplasmic reticulu ...
Homo sapiens
FEBS J.
277
3404-3414
2010
-
-
1
-
8
-
1
-
1
-
-
-
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1
-
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-
-
1
-
-
-
3
-
-
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1
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8
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1
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1
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1
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-
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3
-
-
-
-
-
-
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-
1
1
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-
-
691881
Lugowska
Molecular and clinical consequ ...
Homo sapiens
Clin. Genet.
75
57-64
2009
-
1
1
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3
-
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-
1
-
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1
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2
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1
1
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3
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1
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-
2
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-
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-
693260
Matzner
Saposin B-dependent reconstitu ...
Homo sapiens
J. Biol. Chem.
284
9372-9381
2009
4
-
1
-
-
-
6
-
1
-
-
-
-
1
-
-
-
-
-
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3
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1
1
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4
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1
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6
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1
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-
3
-
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1
1
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-
-
-
-
708623
Schenk
Interaction of arylsulfatase-A ...
Homo sapiens, Sus scrofa
Glycoconj. J.
26
1029-1045
2009
-
-
1
-
2
-
2
-
4
-
-
4
-
2
-
-
-
-
-
4
-
-
12
-
2
-
-
-
2
-
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1
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2
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2
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4
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4
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4
-
-
12
-
2
-
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-
2
-
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-
-
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-
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-
708662
Cesani
Characterization of new arylsu ...
Homo sapiens
Hum. Mutat.
30
E936-E945
2009
-
-
1
-
9
-
-
-
1
-
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1
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1
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9
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1
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-
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-
-
-
-
-
-
2
2
-
-
-
692081
Anupriwan
Presence of arylsulfatase A an ...
Mus musculus
Endocrinology
149
3942-3951
2008
-
-
-
-
-
-
-
-
1
-
1
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-
1
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2
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1
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1
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1
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2
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3
-
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-
1
-
-
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-
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-
-
-
-
675681
Bhattacharyya
Steroid sulfatase, arylsulfata ...
Homo sapiens
J. Steroid Biochem. Mol. Biol.
103
20-34
2007
-
-
-
-
-
-
3
-
-
-
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1
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6
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1
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6
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1
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680078
Capotondo
Safety of arylsulfatase A over ...
Homo sapiens
Hum. Gene Ther.
18
821-836
2007
-
1
1
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1
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2
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1
1
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2
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-
681035
Wu
Sperm surface arylsulfatase A ...
Mus musculus, Sus scrofa
J. Cell. Physiol.
213
201-211
2007
-
2
-
-
1
-
3
-
-
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1
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2
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1
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3
3
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4
1
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2
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1
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3
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1
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3
3
-
4
1
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-
667108
Bognar
Kinetics and activity of aryls ...
Homo sapiens
Acta Pharm.
56
95-104
2006
-
-
-
-
-
-
-
1
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-
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1
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1
1
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1
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1
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1
1
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1
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676996
Kasinathan
Tyrosine sulfation of arylsulf ...
Homo sapiens
Protein Pept. Lett.
13
357-361
2006
-
1
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1
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1
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1
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677433
Jean
Ethanol decreases rat hepatic ...
Rattus norvegicus
Alcohol. Clin. Exp. Res.
30
1950-1955
2006
-
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2
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2
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1
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677796
Jimenez
Carbohydrate affinity chromato ...
Sus scrofa
Arch. Androl.
52
455-462
2006
-
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1
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3
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2
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1
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1
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668874
Takakusaki
Coexpression of formylglycine- ...
Homo sapiens
Hum. Gene Ther.
16
929-936
2005
-
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1
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-
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1
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5
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1
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1
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5
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1
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670343
Luca
Axons mediate the distribution ...
Homo sapiens
Mol. Ther.
12
669-679
2005
-
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1
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1
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4
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1
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1
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4
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1
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670492
Oner-Iyidogan
Evaluation of leukocyte arylsu ...
Homo sapiens
Pharmacol. Res.
52
340-345
2005
-
-
-
-
-
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1
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1
3
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1
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1
3
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1
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652538
Heine
Defective endoplasmic reticulu ...
Homo sapiens
J. Biol. Chem.
279
22347-22352
2004
-
-
-
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1
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1
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1
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653357
Yaghootfam
Three novel mutant arylsulfata ...
Homo sapiens
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Sulfatides and arylsulfatase A ...
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Contribution of arylsulfatase ...
Homo sapiens
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Evaluation of leukocyte arylsu ...
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Lead causes human fibroblasts ...
Homo sapiens
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Lewinski
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Crystallization and preliminar ...
Homo sapiens
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135692
Lukatela
Crystal structure of human ary ...
Homo sapiens
Biochemistry
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Arylsulfatase A: relationship ...
Homo sapiens
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Franco
A cluster of sulfatase genes o ...
Homo sapiens
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Glycosylation and phosphorylat ...
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Wojczyk
Purification of rat liver aryl ...
Rattus norvegicus
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Nikolajczyk
Characterization of rabbit tes ...
Oryctolagus cuniculus
Biol. Reprod.
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366-378
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135690
Gadella
Characterization of three aryl ...
Sus scrofa
Biochim. Biophys. Acta
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155-162
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Sarafian
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Large scale purification of py ...
Homo sapiens
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135667
Fluharty
Bile salt activation of cerebr ...
Homo sapiens
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1980
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135684
Waheed
Chemical characterization and ...
Oryctolagus cuniculus
Biochim. Biophys. Acta
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92-101
1980
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135679
Roy
The hydrolysis of ascorbate 2- ...
Bos taurus
Methods Enzymol.
62
42-47
1979
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135683
Farooqui
Isolation, characterization an ...
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1979
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135668
Fischer
The activator of cerebroside-s ...
Homo sapiens
Biochim. Biophys. Acta
528
69-76
1978
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135681
Fluharty
Arylsulfatases A and B from hu ...
Homo sapiens
Methods Enzymol.
50
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2
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135687
Fischer
Enzymic hydrolysis of sulphosp ...
Homo sapiens
Hoppe-Seyler's Z. Physiol. Chem.
359
863-866
1978
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Helwig
Purification and some properti ...
Oryctolagus cuniculus
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1977
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135669
Stevens
Microheterogeneity of arylsulf ...
Homo sapiens
Biochim. Biophys. Acta
445
661-671
1976
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135671
Mraz
The activator of human cerebro ...
Homo sapiens, Maja squinado, Marthasterias glacialis, Microcosmus sulcatus, Patella vulgata, Tethya aurantium
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357
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1976
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5
-
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
1
-
-
6
-
-
-
5
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
135672
Mraz
The activator of cerebroside s ...
Homo sapiens, Rattus norvegicus
Hoppe-Seyler's Z. Physiol. Chem.
357
1181-1191
1976
2
-
-
-
-
-
-
-
2
-
-
2
-
2
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
2
-
-
2
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
135673
Mraz
Cerebroside sulphatase activit ...
Microcosmus sulcatus, Tethya aurantium
Hoppe-Seyler's Z. Physiol. Chem.
357
1193-1195
1976
2
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
2
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
135695
Roy
Sulphatases, lysosomes and dis ...
Bos taurus, Homo sapiens, Oryctolagus cuniculus
Aust. J. Exp. Biol. Med. Sci.
54
111-135
1976
-
2
-
-
-
-
1
-
-
-
4
3
-
3
-
-
-
-
-
3
-
-
6
2
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
-
4
3
-
-
-
-
-
3
-
-
6
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
135670
Fischer
The activator of cerebroside s ...
Homo sapiens
Hoppe-Seyler's Z. Physiol. Chem.
356
605-613
1975
2
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
1
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
1
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
135676
Stinshoff
Comparison of the cerebroside ...
Homo sapiens
Biochim. Biophys. Acta
377
126-138
1975
6
1
-
-
-
-
7
1
1
-
-
1
-
1
-
-
-
-
-
2
-
-
3
-
-
-
-
-
2
1
-
-
-
-
-
6
1
-
-
-
-
-
-
7
-
1
1
-
-
1
-
-
-
-
-
2
-
-
3
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
135686
Stevens
Purification and properties of ...
Homo sapiens
J. Biol. Chem.
250
2495-2501
1975
1
-
-
-
-
2
-
3
1
1
2
1
-
1
-
-
1
-
-
1
-
3
2
1
-
-
-
3
3
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
-
3
1
1
2
1
-
-
-
1
-
1
-
3
2
1
-
-
-
3
3
-
-
-
-
-
-
-
-
-
135675
Mraz
Cerebroside sulphatase activit ...
Actinia equina, Blaberus fuscus, Helix pomatia, Limaria tuberculata, Lumbricus terrestris, Marthasterias glacialis, Microcosmus sulcatus, Patella vulgata, Pecten jacobaeus, Tethya aurantium
Hoppe-Seyler's Z. Physiol. Chem.
355
33-44
1974
20
-
-
-
-
-
30
10
-
-
-
10
-
10
-
-
-
10
-
-
-
10
20
-
-
-
2
-
5
-
-
-
-
-
-
20
-
-
-
-
-
-
-
30
-
10
-
-
-
10
-
-
-
-
-
-
-
10
20
-
-
-
2
-
5
-
-
-
-
-
-
-
-
-
135677
Fluharty
Sulfoglycerogalactolipid from ...
Homo sapiens
Biochem. Biophys. Res. Commun.
61
348-354
1974
1
-
-
-
-
-
1
1
1
1
-
1
-
1
-
-
1
-
-
1
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
1
1
1
-
1
-
-
-
1
-
1
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
135674
Jefrey
The sulphatase of ox liver. XV ...
Bos taurus
Biochim. Biophys. Acta
293
178-190
1973
1
-
-
-
-
-
2
1
1
1
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
1
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
1
1
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
135678
Porter
Cerebroside sulfatase determin ...
Homo sapiens
Biochim. Biophys. Acta
258
769-778
1972
3
1
-
-
-
-
10
1
-
4
-
1
-
2
-
-
-
-
-
2
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
3
1
-
-
-
-
-
-
10
-
1
-
4
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
135685
Farooqui
Purification and properties of ...
Gallus gallus
Biochem. J.
126
1025-1033
1972
-
-
-
-
-
-
9
-
1
-
-
1
-
1
-
-
1
1
-
1
1
1
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
9
-
-
1
-
-
1
-
-
-
1
-
1
1
1
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
135554
Nicholls
-
Arylsulfatases ...
Bos taurus
The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )
5
21-41
1971
-
-
-
-
-
-
7
12
-
-
-
1
-
1
-
1
-
1
-
-
-
-
13
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
12
-
-
-
1
-
-
1
-
-
-
-
-
13
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
135680
Mehl
Cerebroside 3-sulfate as a phy ...
Sus scrofa
Biochim. Biophys. Acta
151
619-627
1968
-
-
-
-
-
-
5
1
1
-
-
1
-
1
-
-
-
-
-
1
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
1
1
-
-
1
-
-
-
-
-
1
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-