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Literature summary for 3.1.6.4 extracted from

  • Tomatsu, S.; Montano, A.M.; Gutierrez, M.; Grubb, J.H.; Oikawa, H.; Dung, V.C.; Ohashi, A.; Nishioka, T.; Yamada, M.; Yamada, M.; Tosaka, Y.; Trandafirescu, G.G.; Orii, T.
    Characterization and pharmacokinetic study of recombinant human N-acetylgalactosamine-6-sulfate sulfatase (2007), Mol. Genet. Metab., 91, 69-78.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in chinese hamster ovary cells Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
2 * 60000 Da Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
two-step affinity chromatography, purified 1317-fold, 71% yield Homo sapiens

Storage Stability

Storage Stability Organism
4°C, 25 mM sodium acetate, 1 mM beta-glycerophosphate buffer, pH 5.5, several months, no significant loss of acitivity Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetylgalactosamine 6-sulfate + H2O
-
Homo sapiens N-acetylgalactosamine + sulfate
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 60000 Da Homo sapiens

Synonyms

Synonyms Comment Organism
GALNS
-
Homo sapiens
N-acetylgalactosamine-6-sulfate sulfatase recombinant Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
5 6 purified recombinant enzyme Homo sapiens