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Literature summary for 3.1.6.4 extracted from

  • Masue, M.; Sukegawa, K.; Orii, T.; Hashimoto, T.
    N-Acetylgalactosamine-6-sulfate sulfatase in human placenta: purification and characteristics (1991), J. Biochem., 110, 965-970.
    View publication on PubMed

General Stability

General Stability Organism
freezing and thawing five times do not decrease the enzyme activity Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
KCl
-
Homo sapiens
Na2SO4
-
Homo sapiens
NaCl
-
Homo sapiens
NaH2PO4
-
Homo sapiens
phosphate
-
Homo sapiens
SO42-
-
Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.008
-
galactosamine 6-sulfate
-
Homo sapiens
0.013
-
Galactose 6-sulfate
-
Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
15000
-
x * 15000 + x * 40000, the 15000 Da subunit is a glycoprotein, SDS-PAGE Homo sapiens
40000
-
x * 15000 + x * 40000, the 15000 Da subunit is a glycoprotein, SDS-PAGE Homo sapiens
120000
-
gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the 15000 Da subunit is a glycoprotein Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
placenta
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.13
-
-
Homo sapiens

Storage Stability

Storage Stability Organism
-20°C, 2 months, 5% loss of activity Homo sapiens
4°C, 2 months, 20% loss of activity Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-N-acetyl-D-galactosamine-6-sulfate-(1-4)-beta-D-glucuronic acid-(1-3)-N-acetyl-D-galactosaminitol 6-sulfate + H2O
-
Homo sapiens sulfate + ?
-
?
galactosamine 6-sulfate + H2O
-
Homo sapiens galactosamine + sulfate
-
?
galactose 6-sulfate + H2O
-
Homo sapiens galactose + sulfate
-
?
N-acetylgalactosamine 6-sulfate + H2O
-
Homo sapiens N-acetylgalactosamine + sulfate
-
?

Subunits

Subunits Comment Organism
? x * 15000 + x * 40000, the 15000 Da subunit is a glycoprotein, SDS-PAGE Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.8 4 hydrolysis of N-acetylgalactosamine 6-sulfate or galactose 6-sulfate Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
3.3 4.2 pH 3.0: about 45% of maximal activity, pH 4.2: about 55% of maximal activity, hydrolysis of N-acetylgalactosamine 6-sulfate, 0.02 M acetate buffer Homo sapiens