BRENDA - Enzyme Database show
show all sequences of 3.1.6.3

A glycosulphatase that removes sulphate from mucus glycoprotein

Roberton, A.M.; McKenzie, C.G.; Sharff, N.; Stubbs, L.B.; Biochem. J. 293, 683-689 (1993)
No PubMed abstract available

Data extracted from this reference:

General Stability
General Stability
Organism
unstable to freezing
Prevotella sp.
Inhibitors
Inhibitors
Commentary
Organism
Structure
HgCl2
weak
Prevotella sp.
additional information
no inhibition by iodacetamide
Prevotella sp.
p-hydroxymercuribenzoate
-
Prevotella sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
60
-
glucose 6-sulfate
-
Prevotella sp.
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
periplasm
-
Prevotella sp.
-
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
58000
-
2 * 58000, SDS-PAGE
Prevotella sp.
111000
-
gel filtration
Prevotella sp.
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Prevotella sp.
-
-
-
Prevotella sp. RS2
-
-
-
Purification (Commentary)
Commentary
Organism
-
Prevotella sp.
Source Tissue
Source Tissue
Commentary
Organism
Textmining
colon
-
Prevotella sp.
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.9
-
-
Prevotella sp.
Storage Stability
Storage Stability
Organism
4°C, 20 mM phosphate buffer, pH 7.4, 2-mercaptoethanol, 1-3 months, 92-74% of original activity
Prevotella sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-glucose-6-sulfate + H2O
-
135639
Prevotella sp.
D-glucose + sulfate
-
-
-
?
D-glucose-6-sulfate + H2O
-
135639
Prevotella sp. RS2
D-glucose + sulfate
-
-
-
?
N-acetyl-D-glucosamine 6-sulfate + H2O
-
135639
Prevotella sp.
N-acetyl-D-glucosamine + sulfate
-
-
-
?
N-acetyl-D-glucosamine 6-sulfate + H2O
-
135639
Prevotella sp. RS2
N-acetyl-D-glucosamine + sulfate
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 58000, SDS-PAGE
Prevotella sp.
General Stability (protein specific)
General Stability
Organism
unstable to freezing
Prevotella sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
HgCl2
weak
Prevotella sp.
additional information
no inhibition by iodacetamide
Prevotella sp.
p-hydroxymercuribenzoate
-
Prevotella sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
60
-
glucose 6-sulfate
-
Prevotella sp.
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
periplasm
-
Prevotella sp.
-
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
58000
-
2 * 58000, SDS-PAGE
Prevotella sp.
111000
-
gel filtration
Prevotella sp.
Purification (Commentary) (protein specific)
Commentary
Organism
-
Prevotella sp.
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
colon
-
Prevotella sp.
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.9
-
-
Prevotella sp.
Storage Stability (protein specific)
Storage Stability
Organism
4°C, 20 mM phosphate buffer, pH 7.4, 2-mercaptoethanol, 1-3 months, 92-74% of original activity
Prevotella sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-glucose-6-sulfate + H2O
-
135639
Prevotella sp.
D-glucose + sulfate
-
-
-
?
D-glucose-6-sulfate + H2O
-
135639
Prevotella sp. RS2
D-glucose + sulfate
-
-
-
?
N-acetyl-D-glucosamine 6-sulfate + H2O
-
135639
Prevotella sp.
N-acetyl-D-glucosamine + sulfate
-
-
-
?
N-acetyl-D-glucosamine 6-sulfate + H2O
-
135639
Prevotella sp. RS2
N-acetyl-D-glucosamine + sulfate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 58000, SDS-PAGE
Prevotella sp.
Other publictions for EC 3.1.6.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
655716
Macfarlane
Toxin synthesis and mucin brea ...
Clostridium septicum
Infect. Immun.
69
1120-1126
2001
-
-
-
-
-
-
1
-
-
-
-
-
-
5
-
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1
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1
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1
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
135644
Robertson
Bacterial glycosulphatases and ...
Bacteroides thetaiotaomicron, Helicobacter pylori, Prevotella sp., Ruminococcus torques, Ruminococcus torques IX-70
Can. J. Gastroenterol.
11
361-366
1997
-
-
-
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2
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6
-
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8
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5
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2
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-
8
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
135639
Roberton
-
A glycosulphatase that removes ...
Prevotella sp., Prevotella sp. RS2
Biochem. J.
293
683-689
1993
-
-
-
-
-
1
3
1
1
-
2
-
-
3
-
-
1
-
-
1
1
1
4
1
-
-
-
-
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-
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-
1
-
3
-
1
1
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2
-
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-
1
-
1
1
1
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
135641
Slomiany
Glycosulfatase activity of Por ...
Porphyromonas gingivalis
Biochem. Mol. Biol. Int.
29
973-980
1993
-
-
-
-
-
-
-
-
1
-
1
-
-
3
-
-
1
-
-
-
-
-
2
1
-
-
-
-
1
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1
-
1
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1
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-
2
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
135643
Slomiany
Glycosulfatase activity of Hel ...
Helicobacter pylori
J. Physiol. Pharmacol.
44
7-16
1993
2
-
-
-
-
-
1
-
1
-
1
-
-
3
-
-
-
-
-
-
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-
3
1
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-
-
1
-
-
-
-
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-
2
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
3
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
135640
Corfield
Mucin degradation in the human ...
Bifidobacterium sp., Bifidobacterium sp. VIII-210, Bifidobacterium sp. VIII-240, Ruminococcus torques, Ruminococcus torques IX-70, Ruminococcus torques VIII-239
Infect. Immun.
60
3971-3978
1992
-
-
-
-
-
-
-
-
-
-
-
-
-
10
-
-
-
-
-
4
4
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
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-
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-
-
4
4
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
135642
Slomiany
Glycosulfatase activity of Hel ...
Helicobacter pylori
Biochem. Biophys. Res. Commun.
183
506-513
1992
2
-
-
-
-
-
-
-
1
-
-
-
-
3
-
-
-
-
-
1
1
-
6
-
-
-
-
-
1
-
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-
2
-
-
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-
1
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-
-
-
-
-
1
1
-
6
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
135638
Roy
The sulphatase of ox liver. XX ...
Bos taurus
Biochim. Biophys. Acta
704
366-373
1982
-
-
-
-
-
-
-
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1
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1
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1
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1
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1
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-
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-
-
-
135637
McLean
Glycosulphatase from Pseudomon ...
Pseudomonas carrageenovora
Eur. J. Biochem.
101
497-505
1979
-
-
-
-
-
-
1
1
1
-
1
-
-
1
-
-
1
-
-
-
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-
2
1
-
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-
1
1
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-
1
-
1
1
-
1
-
-
-
-
1
-
-
-
-
2
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
135630
Hatanaka
Two glycosulfatases from the l ...
Charonia lampas
J. Biochem.
79
27-34
1976
-
-
-
-
-
-
2
1
-
-
2
-
-
2
-
1
1
-
-
1
1
-
1
-
-
-
-
-
1
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-
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-
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-
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-
-
-
-
-
2
-
1
-
-
2
-
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-
1
1
-
1
1
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
135631
Hatanaka
Arylsulphatase and glycosulpha ...
Charonia lampas
Biochem. J.
159
445-448
1976
-
-
-
-
-
-
-
3
-
-
-
-
-
1
-
-
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-
4
-
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1
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3
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-
4
-
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-
1
-
-
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-
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-
-
-
135632
Hatanaka
Sulfatide and seminolipid as s ...
Charonia lampas
J. Biochem.
78
427-429
1975
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
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1
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-
1
-
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-
-
-
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-
-
-
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-
-
-
135636
Lloyd
-
Desulphation of L-fucose monos ...
Patella vulgata
Biochem. J.
124
21
1971
-
-
-
-
-
-
-
-
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-
-
1
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1
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3
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1
-
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3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
135633
Lloyd
The glycosulphatase of Trichod ...
Trichoderma viride
Biochem. J.
108
393-399
1968
-
-
-
-
-
-
-
-
-
-
-
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-
1
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1
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1
3
-
1
1
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1
1
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1
-
1
3
-
1
1
-
-
1
1
-
-
-
-
-
-
-
-
135635
Lloyd
-
Deactivation of glycosulphatas ...
Patella vulgata
Biochem. J.
107
7-7
1968
-
-
-
-
-
-
-
-
-
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1
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3
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3
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-
135634
Lloyd
-
Separation of glycosulphatase ...
Patella vulgata
Biochem. J.
165
33-34
1967
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2
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1
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1
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3
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1
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3
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135629
Lloyd
-
Glucosulfatases from molluscs ...
Charonia lampas, Littorina littorea
Methods Enzymol.
8
670-677
1966
-
-
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5
2
-
2
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2
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1
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2
1
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7
-
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4
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5
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2
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2
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1
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2
1
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7
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4
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