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Literature summary for 3.1.6.3 extracted from

  • Roberton, A.M.; McKenzie, C.G.; Sharff, N.; Stubbs, L.B.
    A glycosulphatase that removes sulphate from mucus glycoprotein (1993), Biochem. J., 293, 683-689.
No PubMed abstract available
Search for more literature for 3.1.6.3

General Stability

General Stability Organism
unstable to freezing Prevotella sp.

Inhibitors

Inhibitors Comment Organism Structure
HgCl2 weak Prevotella sp.
additional information no inhibition by iodacetamide Prevotella sp.
p-hydroxymercuribenzoate
-
 Prevotella sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
60
-
 glucose 6-sulfate
-
 Prevotella sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
 Prevotella sp.
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
 2 * 58000, SDS-PAGE Prevotella sp.
111000
-
 gel filtration Prevotella sp.

Organism

Organism UniProt Comment Textmining
Prevotella sp.
-
-
-
Prevotella sp. RS2
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Prevotella sp.

Source Tissue

Source Tissue Comment Organism Textmining
colon
-
 Prevotella sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.9
-
-
 Prevotella sp.

Storage Stability

Storage Stability Organism
4°C, 20 mM phosphate buffer, pH 7.4, 2-mercaptoethanol, 1-3 months, 92-74% of original activity Prevotella sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose-6-sulfate + H2O
-
 Prevotella sp. D-glucose + sulfate
-
 ?
D-glucose-6-sulfate + H2O
-
 Prevotella sp. RS2 D-glucose + sulfate
-
 ?
N-acetyl-D-glucosamine 6-sulfate + H2O
-
 Prevotella sp. N-acetyl-D-glucosamine + sulfate
-
 ?
N-acetyl-D-glucosamine 6-sulfate + H2O
-
 Prevotella sp. RS2 N-acetyl-D-glucosamine + sulfate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 58000, SDS-PAGE Prevotella sp.