Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.6.21 extracted from

  • Long, M.; Ruan, L.; Li, F.; Yu, Z.; Xu, X.
    Heterologous expression and characterization of a recombinant thermostable alkylsulfatase (sdsAP) (2011), Extremophiles, 15, 293-301 .
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Urea 100.2% activity at 10 mM Pseudomonas sp. S9

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Pseudomonas sp. S9

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 68.7% residual activity at 10 mM Pseudomonas sp. S9
Cu2+ 82.9% residual activity at 10 mM Pseudomonas sp. S9
EDTA 25.8% residual activity at 10 mM Pseudomonas sp. S9
Fe2+ 55.4% residual activity at 10 mM Pseudomonas sp. S9
Mn2+ 66.1% residual activity at 10 mM Pseudomonas sp. S9
additional information not inhibited by dithiothreitol and 2-mercaptoethanol Pseudomonas sp. S9
Zn2+ 77.8% residual activity at 10 mM Pseudomonas sp. S9

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2643
-
SDS at pH 9.0 and 70°C Pseudomonas sp. S9

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 100.3% activity at 100 mM Pseudomonas sp. S9
additional information not activated by Na+, K+, and Mg2+ Pseudomonas sp. S9
Zn2+ contains zinc Pseudomonas sp. S9

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
147000
-
gel filtration Pseudomonas sp. S9

Organism

Organism UniProt Comment Textmining
Pseudomonas sp. S9 F2WP51
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography Pseudomonas sp. S9

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
23.25
-
purified recombinant enzyme, at pH 9.0 and 70°C Pseudomonas sp. S9

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
SDS + H2O
-
Pseudomonas sp. S9 1-dodecanol + sulfate + Na+
-
?
sodium octyl sulfate + H2O
-
Pseudomonas sp. S9 1-octanol + sulfate + Na+
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 70000, SDS-PAGE Pseudomonas sp. S9
homodimer 2 * 74900, calculated from amino acid sequence Pseudomonas sp. S9

Synonyms

Synonyms Comment Organism
SdsAP
-
Pseudomonas sp. S9

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
Pseudomonas sp. S9

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
the enzyme retains more than 90% activity after incubation at 60 and 65°C for 1 h Pseudomonas sp. S9

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Pseudomonas sp. S9

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 11 the enzyme retains more than 90% activity after incubation at a wide pH range of 6.0-11.0 for 1 h Pseudomonas sp. S9