Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Urea | 100.2% activity at 10 mM | Pseudomonas sp. S9 |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Pseudomonas sp. S9 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 68.7% residual activity at 10 mM | Pseudomonas sp. S9 | |
Cu2+ | 82.9% residual activity at 10 mM | Pseudomonas sp. S9 | |
EDTA | 25.8% residual activity at 10 mM | Pseudomonas sp. S9 | |
Fe2+ | 55.4% residual activity at 10 mM | Pseudomonas sp. S9 | |
Mn2+ | 66.1% residual activity at 10 mM | Pseudomonas sp. S9 | |
additional information | not inhibited by dithiothreitol and 2-mercaptoethanol | Pseudomonas sp. S9 | |
Zn2+ | 77.8% residual activity at 10 mM | Pseudomonas sp. S9 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2643 | - |
SDS | at pH 9.0 and 70°C | Pseudomonas sp. S9 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 100.3% activity at 100 mM | Pseudomonas sp. S9 | |
additional information | not activated by Na+, K+, and Mg2+ | Pseudomonas sp. S9 | |
Zn2+ | contains zinc | Pseudomonas sp. S9 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
147000 | - |
gel filtration | Pseudomonas sp. S9 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. S9 | F2WP51 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography | Pseudomonas sp. S9 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
23.25 | - |
purified recombinant enzyme, at pH 9.0 and 70°C | Pseudomonas sp. S9 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
SDS + H2O | - |
Pseudomonas sp. S9 | 1-dodecanol + sulfate + Na+ | - |
? | |
sodium octyl sulfate + H2O | - |
Pseudomonas sp. S9 | 1-octanol + sulfate + Na+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 70000, SDS-PAGE | Pseudomonas sp. S9 |
homodimer | 2 * 74900, calculated from amino acid sequence | Pseudomonas sp. S9 |
Synonyms | Comment | Organism |
---|---|---|
SdsAP | - |
Pseudomonas sp. S9 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Pseudomonas sp. S9 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
the enzyme retains more than 90% activity after incubation at 60 and 65°C for 1 h | Pseudomonas sp. S9 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Pseudomonas sp. S9 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 11 | the enzyme retains more than 90% activity after incubation at a wide pH range of 6.0-11.0 for 1 h | Pseudomonas sp. S9 |