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Literature summary for 3.1.6.21 extracted from

  • Sun, L.; Chen, P.; Su, Y.; Cai, Z.; Ruan, L.; Xu, X.; Wu, Y.
    Crystal structure of thermostable alkylsulfatase SdsAP from Pseudomonas sp. S9 (2017), Biosci. Rep., 37, BSR20170001 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.6.21

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Pseudomonas sp. S9

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 0.1 M sodium acetate (pH4.5), 0.05 M magnesiumacetate, 20% (w/v) polyethylene glycol 4000 Pseudomonas sp. S9

Protein Variants

Protein Variants Comment Organism
G263A the mutation reduces the enzyme activity for SDS degradation to less than 20% of wild type activity Pseudomonas sp. S9
G263F the mutation reduces the enzyme activity for SDS degradation to less than 20% of wild type activity Pseudomonas sp. S9
Y246A the mutation reduces the enzyme activity for SDS degradation to less than 20% of wild type activity Pseudomonas sp. S9
Y246A/G263A the mutation reduces the enzyme activity for SDS degradation to less than 20% of wild type activity Pseudomonas sp. S9
Y246S the mutation reduces the enzyme activity for SDS degradation to less than 20% of wild type activity Pseudomonas sp. S9
Y246S/G263F completely inactive Pseudomonas sp. S9

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0742
-
 SDS at pH 7.1 and 25°C Pseudomonas sp. S9

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ contains zinc Pseudomonas sp. S9

Organism

Organism UniProt Comment Textmining
Pseudomonas sp. S9 F2WP51
-
-

Purification (Commentary)

Purification (Comment) Organism
IMAC column chromatography, Mono Q column chromatography, and Superdex 200 gel filtration Pseudomonas sp. S9

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
SDS + H2O
-
 Pseudomonas sp. S9 1-dodecanol + sulfate + Na+
-
 ?

Synonyms

Synonyms Comment Organism
primary alkylsulfatase
-
 Pseudomonas sp. S9
SdsAP
-
 Pseudomonas sp. S9

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Pseudomonas sp. S9

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
 the enzyme keeps more than 90% activity after treatment at 65°C for 1 h Pseudomonas sp. S9

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.88
-
 SDS at pH 7.1 and 25°C Pseudomonas sp. S9