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Literature summary for 3.1.6.21 extracted from

  • Pogorevc, M.; Faber, K.
    Purification and characterization of an inverting stereo- and enantioselective sec-alkylsulfatase from the gram-positive bacterium Rhodococcus ruber DSM 44541 (2003), Appl. Environ. Microbiol., 69, 2810-2815 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.6.21

Inhibitors

Inhibitors Comment Organism Structure
additional information addition of EDTA does not eliminate enzyme activity Rhodococcus ruber

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
67000
-
 gel filtration Rhodococcus ruber

Organism

Organism UniProt Comment Textmining
Rhodococcus ruber
-
-
-
Rhodococcus ruber DSM 44541
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-cellulose column chromatography, phenyl Sepharose column chromatography, Q6 column chromatography, Blue Sepharose column chromatography, and Superdex 200 gel filtration Rhodococcus ruber

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.058
-
 cell extract, at pH 7.5 and 30°C Rhodococcus ruber
6.88
-
 after 119fold purification, at pH 7.5 and 30°C Rhodococcus ruber

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+/-)-4-octyl sulfate + H2O
-
 Rhodococcus ruber 4-octanol + sulfate + H+
-
 ?
(+/-)-4-octyl sulfate + H2O
-
 Rhodococcus ruber DSM 44541 4-octanol + sulfate + H+
-
 ?
(R)-2-octyl sulfate + H2O when (+/-)-2-octyl sulfate is used as a substrate, the enzyme shows a clear preference for (R)-2-octyl sulfate Rhodococcus ruber (S)-2-octanol + sulfate + H+
-
 ?
(R)-2-octyl sulfate + H2O when (+/-)-2-octyl sulfate is used as a substrate, the enzyme shows a clear preference for (R)-2-octyl sulfate Rhodococcus ruber DSM 44541 (S)-2-octanol + sulfate + H+
-
 ?
(R)-3-octyl sulfate + H2O when (+/-)-3-octyl sulfate is used as a substrate, the enzyme shows a clear preference for (R)-3-octyl sulfate Rhodococcus ruber 3-octanol + sulfate + H+
-
 ?
(R)-3-octyl sulfate + H2O when (+/-)-3-octyl sulfate is used as a substrate, the enzyme shows a clear preference for (R)-3-octyl sulfate Rhodococcus ruber DSM 44541 3-octanol + sulfate + H+
-
 ?
additional information the enzyme shows no activity with branched and mixed aryl-alkyl sec-sulfates Rhodococcus ruber ?
-
-
additional information the enzyme shows no activity with branched and mixed aryl-alkyl sec-sulfates Rhodococcus ruber DSM 44541 ?
-
-

Subunits

Subunits Comment Organism
monomer 1 * 43000, SDS-PAGE Rhodococcus ruber

Synonyms

Synonyms Comment Organism
RS2
-
 Rhodococcus ruber
sec-alkylsulfatase
-
 Rhodococcus ruber

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Rhodococcus ruber

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 35 about 50% activity at 20°C, about 60% activity at 25°C, 100% activity at 30°C, about 20% activity at 35°C, no activity at 40°C Rhodococcus ruber

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8
-
 Rhodococcus ruber

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9 more than 50% activity between pH 6.5 and 9.0 Rhodococcus ruber

pI Value

Organism Comment pI Value Maximum pI Value
Rhodococcus ruber isoelectric focusing
-
 5.1