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Literature summary for 3.1.6.20 extracted from

  • Sauve, V.; Roversi, P.; Leath, K.; Garman, E.; Antrobus, R.; Lea, S.; Berks, B.
    Mechanism for the hydrolysis of a sulfur-sulfur bond based on the crystal structure of the thiosulfohydrolase SoxB (2009), J. Biol. Chem., 284, 21707-21718 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.6.20

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta 2 (DE3) cells Thermus thermophilus

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with thiosulfate and sulfate, sitting drop vapor diffusion method, using 25% (w/v) PEG 400 as precipitant Thermus thermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ contains Mn2+ Thermus thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[SoxY protein]-S-(2-sulfodisulfanyl)-L-cysteine + H2O Thermus thermophilus
-
 [SoxY protein]-S-disulfanyl-L-cysteine + sulfate
-
 ?
[SoxY protein]-S-sulfosulfanyl-L-cysteine + H2O Thermus thermophilus
-
 [SoxY protein]-S-sulfanyl-L-cysteine + sulfate
-
 ?

Organism

Organism UniProt Comment Textmining
Thermus thermophilus Q72IT0
-
-

Purification (Commentary)

Purification (Comment) Organism
Strep-Tactin Superflow column chromatography and Superdex 200 gel filtration Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
[SoxY protein]-S-(2-sulfodisulfanyl)-L-cysteine + H2O
-
 Thermus thermophilus [SoxY protein]-S-disulfanyl-L-cysteine + sulfate
-
 ?
[SoxY protein]-S-sulfosulfanyl-L-cysteine + H2O
-
 Thermus thermophilus [SoxY protein]-S-sulfanyl-L-cysteine + sulfate
-
 ?

Subunits

Subunits Comment Organism
monomer
-
 Thermus thermophilus

Synonyms

Synonyms Comment Organism
SoxB
-
 Thermus thermophilus
thiosulfohydrolase
-
 Thermus thermophilus