Literature summary for 3.1.6.2 extracted from

Stevenson, B.J.; Waller, C.C.; Ma, P.; Li, K.; Cawley, A.T.; Ollis, D.L.; McLeod, M.D.
Pseudomonas aeruginosa arylsulfatase a purified enzyme for the mild hydrolysis of steroid sulfates (2015), Drug Test. Anal., 7, 903-911 .

Search for more literature for 3.1.6.2

Application

Application	Comment	Organism
analysis	enzyme hydrolyzes dehydroepiandrosterone 3-sulfate and epiandrosterone 3-sulfate in a urine matrix. Purified arylsulfatase contains only sulfatase activity allowing for the selective hydrolysis of sulfate esters in the presence of glucuronide conjugates. The enzyme can be employed for short three-step chemoenzymatic synthesis of 5alpha-androstane-3beta,17beta-diol 17-glucuronide from epiandrosterone 3-sulfate	Pseudomonas aeruginosa

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.007	-	4-nitrophenyl sulfate	pH 8.8, 37°C	Pseudomonas aeruginosa
0.014	-	Estrone 3-sulfate	pH 8.8, 37°C	Pseudomonas aeruginosa
0.026	-	epiandrosterone 3-sulfate	pH 8.8, 37°C	Pseudomonas aeruginosa
0.05	-	testosterone 17-sulfate	pH 8.8, 37°C	Pseudomonas aeruginosa

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas aeruginosa	P51691	cf. EC 3.1.6.1	-
Pseudomonas aeruginosa DSM 22644	P51691	cf. EC 3.1.6.1	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant protein	Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl sulfate + H2O	-	Pseudomonas aeruginosa	4-nitrophenol + sulfate	-	?
androsterone 3-sulfate + H2O	-	Pseudomonas aeruginosa	androsterone + sulfate	-	?
boldenone 17-sulfate + H2O	-	Pseudomonas aeruginosa	boldenone + sulfate	-	?
dehydroepiandrosterone 3-sulfate + H2O	-	Pseudomonas aeruginosa	dehydroepiandrosterone + sulfate	-	?
epiandrosterone 3-sulfate + H2O	-	Pseudomonas aeruginosa	epiandrosterone + sulfate	-	?
epitestosterone 17-sulfate + H2O	-	Pseudomonas aeruginosa	epitestosterone + sulfate	-	?
estrone 3-sulfate + H2O	-	Pseudomonas aeruginosa	estrone + sulfate	-	?
etiocholanolone 3-sulfate + H2O	-	Pseudomonas aeruginosa	etiocholanolone + sulfate	-	?
testosterone 17-sulfate + H2O	-	Pseudomonas aeruginosa	testosterone + sulfate	-	?

Synonyms

Synonyms	Comment	Organism
AtsA	-	Pseudomonas aeruginosa

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Release 2023.1 (January 2023)