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Literature summary for 3.1.6.2 extracted from

  • Hernandez-Guzman, F.G.; Higashiyama, T.; Osawa, Y.; Ghosh, D.
    Purification, characterization and crystallization of human placental estrone/dehydroepiandrosterone sulfatase, a membrane-bound enzyme of the endoplasmic reticulum (2001), J. Steroid Biochem. Mol. Biol., 78, 441-450.
    View publication on PubMed
Search for more literature for 3.1.6.2

Application

Application Comment Organism
medicine X-linked ichthyosis is ascribed to the non-functional ES resulting from point and/or deletion mutations in the ES gene Homo sapiens
pharmacology enzyme inhibitors can be used as anti-tumor agents Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
full-length, active enzyme form, X-ray analysis Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00959
-
 dehydroepiandrosterone sulfate pH 8, 37°C Homo sapiens
0.07275
-
 Estrone sulfate pH 8, 37°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum membrane-bound Homo sapiens 5783
-
membrane bound to the membrane of the endoplasmic reticulum Homo sapiens 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 native PAGE analysis of solubilized single crystals of ES shows all possible oligomeric states between a monomer and a tetramer, but predominantly the monomeric and dimeric states Homo sapiens
65000
-
 x * 65000, SDS-PAGE Homo sapiens
250000
-
 gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens ES is a component of the three-enzyme system that has been implicated in intracrine biosynthesis of estradiol, hence, proliferation of hormone-dependent breast tumors ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein N-linked glycosylation at two of the possible four sites Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
placenta
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Homo sapiens
1.556
-
 pH 8, 37°C, hydrolysis of dehydroepiandrosterone sulfate Homo sapiens

Storage Stability

Storage Stability Organism
room temperature, crystalline state of enzyme, more than 30 days, less than 33% loss of specific activity Homo sapiens
room temperature, n-octyl-beta-D-glucopyranoside, several weeks, nearly stable Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dehydroepiandrosterone sulfate + H2O
-
 Homo sapiens dehydroepiandrosterone + sulfate
-
 ?
estrone sulfate + H2O
-
 Homo sapiens estrone + sulfate
-
 ?
additional information ES is a component of the three-enzyme system that has been implicated in intracrine biosynthesis of estradiol, hence, proliferation of hormone-dependent breast tumors Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
? x * 65000, SDS-PAGE Homo sapiens
More all oligomeric states between a monomer and a tetramer are possible, varying aggregation properties of enzyme under various buffer, pH, detergent and ionic strength conditions, gel filtration suggests ES to be predominantly a homotetramer, native PAGE analysis of solubilized single crystals of ES shows all possible oligomeric states between a monomer and a tetramer, but predominantly the monomeric and dimeric states Homo sapiens

Synonyms

Synonyms Comment Organism
DHEAS
-
 Homo sapiens
ES
-
 Homo sapiens
estrone sulfatase
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.48
-
 dehydroepiandrosterone sulfate pH 8, 37°C Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Homo sapiens