Application | Comment | Organism |
---|---|---|
synthesis | stereoselctive organic synthesis, stereoselective and enantioselective biohydrolysis of sulfate esters of sec-alcohols | Rhodococcus ruber |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
1 * 43000, SDS-PAGE | Rhodococcus ruber |
67000 | - |
gel filtration | Rhodococcus ruber |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus ruber | - |
- |
- |
Rhodococcus ruber DSM 44541 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rhodococcus ruber |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.9 | - |
pH 7.5, 30°C | Rhodococcus ruber |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R)-octan-2-yl sulfate + H2O | enantioselectivity expressed by E-value is 21. The preferred substrates for the enzyme are linear sec-alkyl sulfate esters, particularly 2-, 3-, and 4-octyl sulfates. The enzymatic hydrolysis proceeds through inversion of the configuration at the stereogenic carbon atom | Rhodococcus ruber | (2S)-octan-2-ol + sulfate | - |
? | |
(2R)-octan-2-yl sulfate + H2O | enantioselectivity expressed by E-value is 21. The preferred substrates for the enzyme are linear sec-alkyl sulfate esters, particularly 2-, 3-, and 4-octyl sulfates. The enzymatic hydrolysis proceeds through inversion of the configuration at the stereogenic carbon atom | Rhodococcus ruber DSM 44541 | (2S)-octan-2-ol + sulfate | - |
? | |
additional information | when rather symmetrical molecules, such as 3- or 4-octyl sulfate, are used, the chiral recognition process gets more difficult as the relative sizes of the two alkyl groups adjacent to the stereogenic sulfate ester group become more similar. | Rhodococcus ruber | ? | - |
? | |
additional information | when rather symmetrical molecules, such as 3- or 4-octyl sulfate, are used, the chiral recognition process gets more difficult as the relative sizes of the two alkyl groups adjacent to the stereogenic sulfate ester group become more similar. | Rhodococcus ruber DSM 44541 | ? | - |
? | |
rac-octan-3-yl sulfate + H2O | enantioselectivity expressed by E-value is 4.3. The preferred substrates for the enzyme are linear sec-alkyl sulfate esters, particularly 2-, 3-, and 4-octyl sulfates. The enzymatic hydrolysis proceeds through inversion of the configuration at the stereogenic carbon atom | Rhodococcus ruber | (3S)-octan-3-ol + sulfate | - |
? | |
rac-octan-3-yl sulfate + H2O | enantioselectivity expressed by E-value is 4.3. The preferred substrates for the enzyme are linear sec-alkyl sulfate esters, particularly 2-, 3-, and 4-octyl sulfates. The enzymatic hydrolysis proceeds through inversion of the configuration at the stereogenic carbon atom | Rhodococcus ruber DSM 44541 | (3S)-octan-3-ol + sulfate | - |
? | |
rac-octan-4-yl sulfate + H2O | no enantioselectivity is observed, production of racemic 4-octanol. The preferred substrates for the enzyme are linear sec-alkyl sulfate esters, particularly 2-, 3-, and 4-octyl sulfates. The enzymatic hydrolysis proceeds through inversion of the configuration at the stereogenic carbon atom | Rhodococcus ruber | (4S)-octan-4-ol + sulfate | - |
? | |
rac-octan-4-yl sulfate + H2O | no enantioselectivity is observed, production of racemic 4-octanol. The preferred substrates for the enzyme are linear sec-alkyl sulfate esters, particularly 2-, 3-, and 4-octyl sulfates. The enzymatic hydrolysis proceeds through inversion of the configuration at the stereogenic carbon atom | Rhodococcus ruber DSM 44541 | (4S)-octan-4-ol + sulfate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 43000, SDS-PAGE | Rhodococcus ruber |
Synonyms | Comment | Organism |
---|---|---|
sulfatase RS2 | - |
Rhodococcus ruber |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Rhodococcus ruber |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 30 | 20°C: about 50% of maximal activity, 30°C: optimum, 35°C: about 25% of maximal activity, no activity at 40°C | Rhodococcus ruber |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | - |
Rhodococcus ruber |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9 | pH 6.5: about 55% of maximal activity, pH 9.0: about 50% of maximal activity | Rhodococcus ruber |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Rhodococcus ruber | isoelectric focusing | - |
5.1 |