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Literature summary for 3.1.6.19 extracted from
- Purification and characterization of an inverting stereo- and enantioselective sec-alkylsulfatase from the gram-positive bacterium Rhodococcus ruber DSM 44541 (2003), Appl. Environ. Microbiol., 69, 2810-2815.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | stereoselctive organic synthesis, stereoselective and enantioselective biohydrolysis of sulfate esters of sec-alcohols | Rhodococcus ruber |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
1 * 43000, SDS-PAGE | Rhodococcus ruber |
| 67000 | - |
gel filtration | Rhodococcus ruber |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodococcus ruber | - |
- |
- |
| Rhodococcus ruber DSM 44541 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rhodococcus ruber |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 6.9 | - |
pH 7.5, 30°C | Rhodococcus ruber |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2R)-octan-2-yl sulfate + H2O | enantioselectivity expressed by E-value is 21. The preferred substrates for the enzyme are linear sec-alkyl sulfate esters, particularly 2-, 3-, and 4-octyl sulfates. The enzymatic hydrolysis proceeds through inversion of the configuration at the stereogenic carbon atom | Rhodococcus ruber | (2S)-octan-2-ol + sulfate | - |
? |  |
| (2R)-octan-2-yl sulfate + H2O | enantioselectivity expressed by E-value is 21. The preferred substrates for the enzyme are linear sec-alkyl sulfate esters, particularly 2-, 3-, and 4-octyl sulfates. The enzymatic hydrolysis proceeds through inversion of the configuration at the stereogenic carbon atom | Rhodococcus ruber DSM 44541 | (2S)-octan-2-ol + sulfate | - |
? | |
| additional information | when rather symmetrical molecules, such as 3- or 4-octyl sulfate, are used, the chiral recognition process gets more difficult as the relative sizes of the two alkyl groups adjacent to the stereogenic sulfate ester group become more similar. | Rhodococcus ruber | ? | - |
? | |
| additional information | when rather symmetrical molecules, such as 3- or 4-octyl sulfate, are used, the chiral recognition process gets more difficult as the relative sizes of the two alkyl groups adjacent to the stereogenic sulfate ester group become more similar. | Rhodococcus ruber DSM 44541 | ? | - |
? | |
| rac-octan-3-yl sulfate + H2O | enantioselectivity expressed by E-value is 4.3. The preferred substrates for the enzyme are linear sec-alkyl sulfate esters, particularly 2-, 3-, and 4-octyl sulfates. The enzymatic hydrolysis proceeds through inversion of the configuration at the stereogenic carbon atom | Rhodococcus ruber | (3S)-octan-3-ol + sulfate | - |
? | |
| rac-octan-3-yl sulfate + H2O | enantioselectivity expressed by E-value is 4.3. The preferred substrates for the enzyme are linear sec-alkyl sulfate esters, particularly 2-, 3-, and 4-octyl sulfates. The enzymatic hydrolysis proceeds through inversion of the configuration at the stereogenic carbon atom | Rhodococcus ruber DSM 44541 | (3S)-octan-3-ol + sulfate | - |
? | |
| rac-octan-4-yl sulfate + H2O | no enantioselectivity is observed, production of racemic 4-octanol. The preferred substrates for the enzyme are linear sec-alkyl sulfate esters, particularly 2-, 3-, and 4-octyl sulfates. The enzymatic hydrolysis proceeds through inversion of the configuration at the stereogenic carbon atom | Rhodococcus ruber | (4S)-octan-4-ol + sulfate | - |
? | |
| rac-octan-4-yl sulfate + H2O | no enantioselectivity is observed, production of racemic 4-octanol. The preferred substrates for the enzyme are linear sec-alkyl sulfate esters, particularly 2-, 3-, and 4-octyl sulfates. The enzymatic hydrolysis proceeds through inversion of the configuration at the stereogenic carbon atom | Rhodococcus ruber DSM 44541 | (4S)-octan-4-ol + sulfate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 43000, SDS-PAGE | Rhodococcus ruber |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| sulfatase RS2 | - |
Rhodococcus ruber |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Rhodococcus ruber |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 30 | 20°C: about 50% of maximal activity, 30°C: optimum, 35°C: about 25% of maximal activity, no activity at 40°C | Rhodococcus ruber |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8 | - |
Rhodococcus ruber |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 9 | pH 6.5: about 55% of maximal activity, pH 9.0: about 50% of maximal activity | Rhodococcus ruber |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Rhodococcus ruber | isoelectric focusing | - |
5.1 |
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