BRENDA - Enzyme Database show
show all sequences of 3.1.6.18

Glucuronate-2-sulphatase activity in cultured human skin fibroblast homogenates

Freeman, C.; Hopwood, J.J.; Biochem. J. 279, 399-405 (1991)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
bovine serum albumin
activation; over pH-range 2.7-5.7, maximal activation at 1 mg/ml and pH 3.3, glucuronic acid 2-sulfate-2,5-anhydro-D-mannitol 6-sulfate as substrate
Homo sapiens
Inhibitors
Inhibitors
Commentary
Organism
Structure
Cu2+
above pH 4.2, activation over pH-range 3-4.2, glucuronic acid 2-sulfate-2,5-anhydro-D-mannitol 6-sulfate as substrate
Homo sapiens
EDTA
glucuronic acid 2-sulfate-2,5-anhydro-D-mannitol 6-sulfate as substrate
Homo sapiens
NaCl
at pH 3, glucuronic acid 2-sulfate-2,5-anhydro-D-mannitol 6-sulfate as substrate; stimulates between pH 4.2-4.8
Homo sapiens
NaH2PO4
glucuronic acid 2-sulfate-2,5-anhydro-D-mannitol 6-sulfate as substrate
Homo sapiens
SO42-
glucuronic acid 2-sulfate-2,5-anhydro-D-mannitol 6-sulfate as substrate; product inhibition
Homo sapiens
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Cu2+
activation; pH-dependent: over pH-range 3-4.2, inhibits at higher pH, glucuronic acid 2-sulfate-2,5-anhydro-D-mannitol 6-sulfate as substrate
Homo sapiens
NaCl
stimulation, over pH-range 4.2-4.8, glucuronic acid 2-sulfate-2,5-anhydro-D-mannitol 6-sulfate as substrate, inhibits at pH 3
Homo sapiens
Zn2+
activation; over pH-range 3-4.8
Homo sapiens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
skin fibroblast
cell culture
Homo sapiens
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-glucuronic acid 2-sulfate-2,5-anhydro-D-mannitol 6-sulfate + H2O
i.e. O-beta-glucuronic acid 2-sulfate-1,4-O-(2,5)-anhydromannitol 6-sulfate
646499
Homo sapiens
D-glucuronic acid 2,5-anhydro-D-mannitol 6-sulfate + SO42-
-
646499
Homo sapiens
?
D-glucuronic acid 2-sulfate-2,5-anhydro-D-mannitol 6-sulfate + H2O
i.e. GSMS
646499
Homo sapiens
D-glucuronic acid 2,5-anhydro-D-mannitol 6-sulfate + SO42-
-
646499
Homo sapiens
?
D-glucuronic acid 2-sulfate-2,5-anhydro-D-talitol 6-sulfate + H2O
i.e. O-beta-glucuronic acid 2-sulfate-1,4-O-(2,5)-anhydrotalitol 6-sulfate
646499
Homo sapiens
D-glucuronic acid 2,5-anhydro-D-talitol 6-sulfate + SO42-
-
-
-
?
D-glucuronic acid 2-sulfate-2,5-anhydro-D-talitol 6-sulfate + H2O
i.e. GSTS
646499
Homo sapiens
D-glucuronic acid 2,5-anhydro-D-talitol 6-sulfate + SO42-
-
-
-
?
additional information
no hydrolysis of iduronate 2-sulfate residues
646499
Homo sapiens
?
-
-
-
-
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
additional information
-
the pH/activity profile depends on buffer composition and ionic strength, particularly at lower pH-values
Homo sapiens
2.7
-
20 mM sodium formate buffer, glucuronic acid 2-sulfate-2,5-anhydro-D-talitol 6-sulfate as substrate
Homo sapiens
3
-
4-20 mM sodium formate or 20-60 mM sodium acetate buffer; glucuronic acid 2-sulfate-2,5-anhydro-D-mannitol 6-sulfate as substrate
Homo sapiens
3.3
-
60 mM sodium formate or 60 mM glycine/HCl buffer, glucuronic acid 2-sulfate-2,5-anhydro-D-mannitol 6-sulfate as substrate
Homo sapiens
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
bovine serum albumin
activation; over pH-range 2.7-5.7, maximal activation at 1 mg/ml and pH 3.3, glucuronic acid 2-sulfate-2,5-anhydro-D-mannitol 6-sulfate as substrate
Homo sapiens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Cu2+
above pH 4.2, activation over pH-range 3-4.2, glucuronic acid 2-sulfate-2,5-anhydro-D-mannitol 6-sulfate as substrate
Homo sapiens
EDTA
glucuronic acid 2-sulfate-2,5-anhydro-D-mannitol 6-sulfate as substrate
Homo sapiens
NaCl
at pH 3, glucuronic acid 2-sulfate-2,5-anhydro-D-mannitol 6-sulfate as substrate; stimulates between pH 4.2-4.8
Homo sapiens
NaH2PO4
glucuronic acid 2-sulfate-2,5-anhydro-D-mannitol 6-sulfate as substrate
Homo sapiens
SO42-
glucuronic acid 2-sulfate-2,5-anhydro-D-mannitol 6-sulfate as substrate; product inhibition
Homo sapiens
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Cu2+
activation; pH-dependent: over pH-range 3-4.2, inhibits at higher pH, glucuronic acid 2-sulfate-2,5-anhydro-D-mannitol 6-sulfate as substrate
Homo sapiens
NaCl
stimulation, over pH-range 4.2-4.8, glucuronic acid 2-sulfate-2,5-anhydro-D-mannitol 6-sulfate as substrate, inhibits at pH 3
Homo sapiens
Zn2+
activation; over pH-range 3-4.8
Homo sapiens
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
skin fibroblast
cell culture
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-glucuronic acid 2-sulfate-2,5-anhydro-D-mannitol 6-sulfate + H2O
i.e. O-beta-glucuronic acid 2-sulfate-1,4-O-(2,5)-anhydromannitol 6-sulfate
646499
Homo sapiens
D-glucuronic acid 2,5-anhydro-D-mannitol 6-sulfate + SO42-
-
646499
Homo sapiens
?
D-glucuronic acid 2-sulfate-2,5-anhydro-D-mannitol 6-sulfate + H2O
i.e. GSMS
646499
Homo sapiens
D-glucuronic acid 2,5-anhydro-D-mannitol 6-sulfate + SO42-
-
646499
Homo sapiens
?
D-glucuronic acid 2-sulfate-2,5-anhydro-D-talitol 6-sulfate + H2O
i.e. O-beta-glucuronic acid 2-sulfate-1,4-O-(2,5)-anhydrotalitol 6-sulfate
646499
Homo sapiens
D-glucuronic acid 2,5-anhydro-D-talitol 6-sulfate + SO42-
-
-
-
?
D-glucuronic acid 2-sulfate-2,5-anhydro-D-talitol 6-sulfate + H2O
i.e. GSTS
646499
Homo sapiens
D-glucuronic acid 2,5-anhydro-D-talitol 6-sulfate + SO42-
-
-
-
?
additional information
no hydrolysis of iduronate 2-sulfate residues
646499
Homo sapiens
?
-
-
-
-
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
additional information
-
the pH/activity profile depends on buffer composition and ionic strength, particularly at lower pH-values
Homo sapiens
2.7
-
20 mM sodium formate buffer, glucuronic acid 2-sulfate-2,5-anhydro-D-talitol 6-sulfate as substrate
Homo sapiens
3
-
4-20 mM sodium formate or 20-60 mM sodium acetate buffer; glucuronic acid 2-sulfate-2,5-anhydro-D-mannitol 6-sulfate as substrate
Homo sapiens
3.3
-
60 mM sodium formate or 60 mM glycine/HCl buffer, glucuronic acid 2-sulfate-2,5-anhydro-D-mannitol 6-sulfate as substrate
Homo sapiens
Other publictions for EC 3.1.6.18
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
646499
Freeman
Glucuronate-2-sulphatase activ ...
Homo sapiens
Biochem. J.
279
399-405
1991
1
-
-
-
-
-
5
-
-
3
-
-
-
1
-
-
-
-
-
1
-
-
5
-
-
-
-
-
4
-
-
-
-
-
-
1
-
-
-
-
-
-
-
5
-
-
-
3
-
-
-
-
-
-
-
1
-
-
5
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
646498
Freeman
Human liver glucuronate 2-sulp ...
Homo sapiens
Biochem. J.
259
209-216
1989
1
-
-
-
-
-
8
-
1
2
1
-
-
1
-
1
1
-
-
4
1
1
6
1
-
-
-
-
2
-
-
-
-
5
-
1
-
-
-
-
-
-
-
8
-
-
1
2
1
-
-
-
1
1
-
4
1
1
6
1
-
-
-
-
2
-
-
5
-
-
-
-
-
-
646497
Shaklee
A sulfatase specific for glucu ...
Gallus gallus, Homo sapiens
J. Biol. Chem.
260
9146-9149
1985
-
-
-
-
-
-
2
-
2
-
2
-
-
3
-
-
-
-
-
3
-
-
8
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
2
-
2
-
-
-
-
-
-
3
-
-
8
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-