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Literature summary for 3.1.6.18 extracted from
- Human liver glucuronate 2-sulphatase. Purification, characterization and catalytic properties (1989), Biochem. J., 259, 209-216.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| bovine serum albumin | activation | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Homo sapiens |  |
| Glucuronic acid 2,5-anhydro-D-mannitol 6-sulfate | product inhibition | Homo sapiens | |
| Glucuronic acid 2-sulfate-2,5-anhydro-D-mannitol 6-sulfate | i.e. GSMS, substrate inhibition, above 0.004 mM | Homo sapiens | |
| Glucuronic acid 2-sulfate-2,5-anhydro-D-talitol 6-sulfate | i.e. GSTS, substrate inhibition, above 0.004 mM | Homo sapiens | |
| NaCl | - |
Homo sapiens | |
| NaH2PO4 | - |
Homo sapiens | |
| O-alpha-Iduronic acid 2-sulfate-1,4-O-(2,5)-anhydro-D-mannitol 6-sulfate | - |
Homo sapiens | |
| SO42- | product inhibition | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| lysosome | - |
Homo sapiens | 5764 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | activation | Homo sapiens | |
| Zn2+ | activation | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 69000 | - |
enzyme from liver, gel filtration | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| liver | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | - |
Homo sapiens | - |
| liver | - |
Homo sapiens | - |
| lung | - |
Homo sapiens | - |
| skin fibroblast | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 6.65 | - |
liver | Homo sapiens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, in 50 mM sodium acetate buffer, pH 5.6, 0.5 M NaCl, partially purified enzyme preparation, 10% loss of activity within 1 month, purified enzyme, t1/2: 48 h, 0.1 mg/ml bovine serum albumin stabilizes | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucuronic acid 2-sulfate-2,5-anhydro-D-mannitol 6-sulfate + H2O | i.e. GSMS | Homo sapiens | D-glucuronic acid 2,5-anhydro-D-mannitol 6-sulfate + SO42- | - |
? | |
| D-glucuronic acid 2-sulfate-2,5-anhydro-D-mannitol 6-sulfate + H2O | i.e. heparan sulfate | Homo sapiens | D-glucuronic acid 2,5-anhydro-D-mannitol 6-sulfate + SO42- | - |
? | |
| D-glucuronic acid 2-sulfate-2,5-anhydro-D-mannitol 6-sulfate + H2O | disaccharide substrate derived from heparin | Homo sapiens | D-glucuronic acid 2,5-anhydro-D-mannitol 6-sulfate + SO42- | - |
? | |
| D-glucuronic acid 2-sulfate-2,5-anhydro-D-talitol 6-sulfate + H2O | i.e. chondroitin sulfate | Homo sapiens | D-glucuronic acid 2,5-anhydro-D-talitol 6-sulfate + SO42- | - |
? | |
| D-glucuronic acid 2-sulfate-2,5-anhydro-D-talitol 6-sulfate + H2O | i.e. GSTS | Homo sapiens | D-glucuronic acid 2,5-anhydro-D-talitol 6-sulfate + SO42- | - |
? | |
| additional information | no hydrolysis of iduronate 2-sulfate residues | Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | - |
Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3 | - |
glucuronic acid 2-sulfate-2,5-anhydro-D-mannitol 6-sulfate as substrate | Homo sapiens |
| 3.2 | - |
glucuronic acid 2-sulfate-2,5-anhydro-D-talitol 6-sulfate as substrate | Homo sapiens |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Homo sapiens | urine enzyme forms with pI: 4.6, 5.2, 5.8 and pI: below 4 | 4.7 | 4.6 |
| Homo sapiens | urine enzyme forms with pI: 4.6, 5.2, 5.8 and pI: below 4 | - |
5.2 |
| Homo sapiens | urine enzyme forms with pI: 4.6, 5.2, 5.8 and pI: below 4 | - |
5.8 |
| Homo sapiens | major enzyme form from human skin | - |
6.5 |
| Homo sapiens | skin fibroblasts, additional enzyme form 5.4, 5.8, below 4 and above 7.5 | - |
6.5 |
| Homo sapiens | above, major enzyme form in liver, more than 90% of liver activity, chromatofocusing | - |
7.5 |
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